Label-free counting of circulating cells by in vivo photoacoustic flow cytometry

2018 ◽  
Author(s):  
Xunbin Wei ◽  
Qiyan Wang ◽  
Quanyu Zhou ◽  
Ping Yang ◽  
Kai Pang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Label Free ◽  
Circulating Cells

scholarly journals Recent advances in fluorescence-basedin vivoflow cytometry

2019 ◽  
Vol 12 (06) ◽  
pp. 1930008
Author(s):  
Kai Pang ◽  
Bobo Gu ◽  
Feng Liu ◽  
Mingli Dong ◽  
Lianqing Zhu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Detection Methods ◽  
Biological Applications ◽  
Label Free ◽  
Circulating Cells ◽  
Recent Advances ◽  
Label Free Detection ◽  
In Vivo Flow Cytometry ◽  
Biological Environment

The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.

Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2015 ◽  
Author(s):  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
Hao He ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals In Vivo Flow Cytometry of Extremely Rare Circulating Cells

2018 ◽  
Author(s):  
Xuefei Tan ◽  
Roshani Patil ◽  
Peter Bartosik ◽  
Judith Runnels ◽  
Charles P. Lin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cancer Metastasis ◽  
Near Infrared ◽  
Small Animal ◽  
Skin Surface ◽  
Signal Processing Algorithm ◽  
Circulating Blood Volume ◽  
Circulating Cells ◽  
In Vivo Flow Cytometry

Circulating tumor cells (CTCs) are of great interest in cancer research, but methods for their enumeration remain far from optimal. We developed a new small animal research tool called Diffuse in vivo Flow Cytometry (DiFC) for detecting extremely rare fluorescently- labeled circulating cells directly in the bloodstream. The technique exploits near-infrared diffuse photons to detect and count cells flowing in large superficial arteries and veins without drawing blood samples. DiFC uses custom-designed, dual fiber optic probes that are placed in contact with the skin surface approximately above a major vascular bundle. In combination with a novel signal processing, algorithm DiFC allows counting of individual cells moving in arterial or venous directions, as well as measurement of their speed and depth. We show that DiFC allows sampling of the entire circulating blood volume of a mouse in under 10 minutes, while maintaining a false alarm rate of 0.014 per minute. Hence, the unique capabilities of DiFC are highly suited to biological applications involving very rare cell types such as the study of hematogenic cancer metastasis.

Label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2016 ◽  
Author(s):  
Xiaoling Wang ◽  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals Real-Time Label-Free Embolus Detection Using In Vivo Photoacoustic Flow Cytometry

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0156269 ◽  
Author(s):  
Mazen A. Juratli ◽  
Yulian A. Menyaev ◽  
Mustafa Sarimollaoglu ◽  
Eric R. Siegel ◽  
Dmitry A. Nedosekin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Label Free

scholarly journals Photoacoustic Flow Cytometry for Single Sickle Cell DetectionIn VitroandIn Vivo

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Chengzhong Cai ◽  
Dmitry A. Nedosekin ◽  
Yulian A. Menyaev ◽  
Mustafa Sarimollaoglu ◽  
Mikhail A. Proskurnin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Real Time ◽  
Sickle Cell ◽  
Blood Cells ◽  
Label Free ◽  
Linear Mode ◽  
Signal Fluctuation

Control of sickle cell disease (SCD) stage and treatment efficiency are still time-consuming which makes well-timed prevention of SCD crisis difficult. We show here thatin vivophotoacoustic (PA) flow cytometry (PAFC) has a potential for real-time monitoring of circulating sickled cells in mouse model.In vivodata were verified byin vitroPAFC and photothermal (PT) and PA spectral imaging of sickle red blood cells (sRBCs) expressing SCD-associated hemoglobin (HbS) compared to normal red blood cells (nRBCs). We discovered that PT and PA signal amplitudes from sRBCs in linear mode were 2–4-fold lower than those from nRBCs. PT and PA imaging revealed more profound spatial hemoglobin heterogeneity in sRBCs than in nRBCs, which can be associated with the presence of HbS clusters with high local absorption. This hypothesis was confirmed in nonlinear mode through nanobubble formation around overheated HbS clusters accompanied by spatially selective signal amplification. More profound differences in absorption of sRBCs than in nRBCs led to notable increase in PA signal fluctuation (fluctuation PAFC mode) as an indicator of SCD. The obtained data suggest that noninvasive label-free fluctuation PAFC has a potential for real-time enumeration of sRBCs bothin vitroandin vivo.

Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

2012 ◽  
Vol 37 (13) ◽  
pp. 2490 ◽  
Author(s):  
Yan Zeng ◽  
Jin Xu ◽  
Dong Li ◽  
Li Li ◽  
Zilong Wen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Autofluorescence Imaging ◽  
Label Free ◽  
Two Photon ◽  
In Vivo Flow Cytometry
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