Chromatin structure quantification from super-resolution intrinsic fluorescence imaging (Conference Presentation)

2018 ◽  
Author(s):  
Yue Li ◽  
Biqin Dong ◽  
Adam Eshein ◽  
Graham Spicer ◽  
Wenli Wu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Chromatin Structure ◽  
Super Resolution ◽  
Intrinsic Fluorescence

scholarly journals Characterization of Locus Specific Chromatin Structure and Dynamics using Correlative Conventional Super Resolution Crispr dCas9/Ms2 Imaging

2021 ◽  
Vol 120 (3) ◽  
pp. 9a
Author(s):  
Dushyant Mehra ◽  
Chiranjib Banerjee ◽  
Santosh Adhikari ◽  
Jacob M. Ritz ◽  
Angel Mancebo ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Super Resolution ◽  
Structure And Dynamics ◽  
Chromatin Structure And Dynamics

Super-resolution fluorescence imaging of biocompatible carbon dots

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 8617 ◽  
Author(s):  
Godefroy Leménager ◽  
Elisa De Luca ◽  
Ya-Ping Sun ◽  
Pier Paolo Pompa
Keyword(s):  
Fluorescence Imaging ◽  
Carbon Dots ◽  
Super Resolution

Super-resolution Fluorescence Imaging for Materials Science

Small Methods ◽  
2017 ◽  
Vol 1 (10) ◽  
pp. 1700191 ◽  
Author(s):  
Dominik Wöll ◽  
Cristina Flors
Keyword(s):  
Fluorescence Imaging ◽  
Materials Science ◽  
Super Resolution

scholarly journals Super-Resolution Fluorescence Imaging Reveals Nanoscale Organization of Stress Granule

2014 ◽  
Vol 106 (2) ◽  
pp. 397a
Author(s):  
Ko Sugawara ◽  
Kohki Okabe ◽  
Akihiko Sakamoto ◽  
Takashi Funatsu
Keyword(s):  
Fluorescence Imaging ◽  
Super Resolution ◽  
Stress Granule

scholarly journals Fluorescence imaging with tailored light

Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2111-2128 ◽  
Author(s):  
Jialei Tang ◽  
Jinhan Ren ◽  
Kyu Young Han
Keyword(s):  
Fluorescence Imaging ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Optical Parameters ◽  
Propagation Angle ◽  
Resolution Imaging ◽  
Time Observation ◽  
Living Organisms ◽  
Real Time Observation

AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

scholarly journals Three-Dimensional, Tomographic Super-Resolution Fluorescence Imaging of Serially Sectioned Thick Samples

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e38098 ◽  
Author(s):  
Siddharth Nanguneri ◽  
Benjamin Flottmann ◽  
Heinz Horstmann ◽  
Mike Heilemann ◽  
Thomas Kuner
Keyword(s):  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Super Resolution

Fast Fourier-Domain Localization Algorithm of Single Molecule with Nanometer Resolution for Super-Resolution Fluorescence Imaging

2012 ◽  
Vol 32 (2) ◽  
pp. 0218001
Author(s):  
于斌 Yu Bin ◽  
陈丹妮 Chen Danni ◽  
刘磊 Liu Lei ◽  
屈军乐 Qu Junle ◽  
牛憨笨 Niu Hanben
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Super Resolution ◽  
Fourier Domain ◽  
Localization Algorithm ◽  
Nanometer Resolution

scholarly journals Coupling chromatin structure and dynamics by live super-resolution imaging

2020 ◽  
Vol 6 (27) ◽  
pp. eaaz2196 ◽  
Author(s):  
R. Barth ◽  
K. Bystricky ◽  
H. A. Shaban
Keyword(s):  
Chromatin Structure ◽  
Cell Function ◽  
Super Resolution ◽  
Motion Reconstruction ◽  
Chromatin Dynamics ◽  
Structure And Dynamics ◽  
Photoactivated Localization Microscopy ◽  
Resolution Imaging ◽  
Insight Into ◽  
Chromatin Structure And Dynamics

Chromatin conformation regulates gene expression and thus, constant remodeling of chromatin structure is essential to guarantee proper cell function. To gain insight into the spatiotemporal organization of the genome, we use high-density photoactivated localization microscopy and deep learning to obtain temporally resolved super-resolution images of chromatin in living cells. In combination with high-resolution dense motion reconstruction, we find elongated ~45- to 90-nm-wide chromatin “blobs.” A computational chromatin model suggests that these blobs are dynamically associating chromatin fragments in close physical and genomic proximity and adopt topologically associated domain–like interactions in the time-average limit. Experimentally, we found that chromatin exhibits a spatiotemporal correlation over ~4 μm in space and tens of seconds in time, while chromatin dynamics are correlated over ~6 μm and last 40 s. Notably, chromatin structure and dynamics are closely related, which may constitute a mechanism to grant access to regions with high local chromatin concentration.

The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging

2010 ◽  
Vol 149 (4) ◽  
pp. 260-266 ◽  
Author(s):  
Sebastian van de Linde ◽  
Steve Wolter ◽  
Mike Heilemann ◽  
Markus Sauer
Keyword(s):  
Fluorescence Imaging ◽  
Super Resolution
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