Handheld optical system for fast and accurate in vivo fluorescence lifetime imaging (FLIM) (Conference Presentation)

2018 ◽  
Author(s):  
Rodrigo Cuenca ◽  
Shuna Cheng ◽  
Beena Ahmed ◽  
Kristen C. Maitland ◽  
Javier A. Jo
Keyword(s):  
Fluorescence Lifetime ◽  
Optical System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

scholarly journals In Vivo Fluorescence Lifetime Imaging for Monitoring the Efficacy of the Cancer Treatment

2014 ◽  
Vol 20 (13) ◽  
pp. 3531-3539 ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Victor Chernomordik ◽  
Moinuddin Hassan ◽  
Rafal Zielinski ◽  
Jacek Capala ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

Monitoring the efficacy of targeted therapy with in vivo fluorescence lifetime imaging

2014 ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Victor Chernomordik ◽  
Moinuddin Hassan ◽  
Rafal Zielinski ◽  
Jaleh Shojaeian ◽  
...  
Keyword(s):  
Targeted Therapy ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

scholarly journals Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Dan L. Sackett ◽  
Jay R. Knutson ◽  
Amir H. Gandjbakhche
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Her2 Positive ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

Detection of Specific Binding to HER2 Receptors by in vivo Fluorescence Lifetime Imaging

2012 ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Victor Chernomordik ◽  
Moinuddin Hassan ◽  
Rafal Zielinski ◽  
Gary Griffiths ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Specific Binding ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

scholarly journals In Vivo Fluorescence Lifetime Imaging Monitors Binding of Specific Probes to Cancer Biomarkers

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31881 ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Victor Chernomordik ◽  
Rafal Zielinski ◽  
Jacek Capala ◽  
Gary Griffiths ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Cancer Biomarkers ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

scholarly journals Coarse-grained modeling of mitochondrial metabolism enables subcellular flux inference from fluorescence lifetime imaging microscopy

2020 ◽  
Author(s):  
Xingbo Yang ◽  
Daniel J. Needleman
Keyword(s):  
Fluorescence Lifetime ◽  
Metabolic Flux ◽  
Living Cells ◽  
Coarse Grained ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Metabolic Fluxes ◽  
Subcellular Resolution

AbstractMitochondria are central to metabolism and their dysfunctions are associated with many diseases1–9. Metabolic flux, the rate of turnover of molecules through a metabolic pathway, is one of the most important quantities in metabolism, but it remains a challenge to measure spatiotemporal variations in mitochondrial metabolic fluxes in living cells. Fluorescence lifetime imaging microscopy (FLIM) of NADH is a label-free technique that is widely used to characterize the metabolic state of mitochondria in vivo10–18. However, the utility of this technique has been limited by the inability to relate FLIM measurement to the underlying metabolic activities in mitochondria. Here we show that, if properly interpreted, FLIM of NADH can be used to quantitatively measure the flux through a major mitochondrial metabolic pathway, the electron transport chain (ETC), in vivo with subcellular resolution. This result is based on the use of a coarse-grained NADH redox model, which we test in mouse oocytes subject to a wide variety of perturbations by comparing predicted fluxes to direct biochemical measurements and by self-consistency criterion. Using this method, we discovered a subcellular spatial gradient of mitochondrial metabolic flux in mouse oocytes. We showed that this subcellular variation in mitochondrial flux correlates with a corresponding subcellular variation in mitochondrial membrane potential. The developed model, and the resulting procedure for analyzing FLIM of NADH, are valid under nearly all circumstances of biological interest. Thus, this approach is a general procedure to measure metabolic fluxes dynamically in living cells, with subcellular resolution.

Towards in-vivo assessment of fluorescence lifetime: imaging using time-gated intensified CCD camera

2018 ◽  
Vol 38 (4) ◽  
pp. 966-974 ◽  
Author(s):  
Piotr Sawosz ◽  
Stanislaw Wojtkiewicz ◽  
Michal Kacprzak ◽  
Elzbieta Zieminska ◽  
Magdalena Morawiec ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Ccd Camera ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Assessment
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