Intraoperative determination of tumor aggressiveness by real-time label-free nonlinear imaging and characterization of tumor-associated extracellular vesicles (Conference Presentation)

2018 ◽  
Author(s):  
Yi Sun ◽  
Sixian You ◽  
Haohua Tu ◽  
Darold R. Spillman ◽  
Marina Marjanovic ◽  
...  
Keyword(s):  
Real Time ◽  
Extracellular Vesicles ◽  
Label Free ◽  
Tumor Aggressiveness ◽  
Nonlinear Imaging

scholarly journals Intraoperative visualization of the tumor microenvironment and quantification of extracellular vesicles by label-free nonlinear imaging

2018 ◽  
Vol 4 (12) ◽  
pp. eaau5603 ◽  
Author(s):  
Yi Sun ◽  
Sixian You ◽  
Haohua Tu ◽  
Darold R. Spillman ◽  
Eric J. Chaney ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Extracellular Vesicles ◽  
Cancer Progression ◽  
Spatial Information ◽  
Imaging System ◽  
Label Free ◽  
Breast Reduction Surgery ◽  
Diagnosis And Prognosis ◽  
Nonlinear Imaging

Characterization of the tumor microenvironment, including extracellular vesicles (EVs), is important for understanding cancer progression. EV studies have traditionally been performed on dissociated cells, lacking spatial information. Since the distribution of EVs in the tumor microenvironment is associated with cellular function, there is a strong need for visualizing EVs in freshly resected tissues. We intraoperatively imaged untreated human breast tissues using a custom nonlinear imaging system. Label-free optical contrasts of the tissue, correlated with histological findings, enabled point-of-procedure characterization of the tumor microenvironment. EV densities from 29 patients with breast cancer were found to increase with higher histologic grade and shorter tumor-to-margin distance and were significantly higher than those from 7 cancer-free patients undergoing breast reduction surgery. Acquisition and interpretation of these intraoperative images not only provide real-time visualization of the tumor microenvironment but also offer the potential to use EVs as a label-free biomarker for cancer diagnosis and prognosis.

scholarly journals Label-Free Prostate Cancer Detection by Characterization of Extracellular Vesicles Using Raman Spectroscopy

2018 ◽  
Vol 90 (19) ◽  
pp. 11290-11296 ◽  
Author(s):  
Wooje Lee ◽  
Afroditi Nanou ◽  
Linda Rikkert ◽  
Frank A. W. Coumans ◽  
Cees Otto ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Raman Spectroscopy ◽  
Extracellular Vesicles ◽  
Cancer Detection ◽  
Label Free ◽  
Prostate Cancer Detection

A Review of Electrical Impedance Characterization of Cells for Label-Free and Real-Time Assays

2019 ◽  
Vol 13 (4) ◽  
pp. 295-305 ◽  
Author(s):  
Hien T. Ngoc Le ◽  
Junsub Kim ◽  
Jinsoo Park ◽  
Sungbo Cho
Keyword(s):  
Real Time ◽  
Electrical Impedance ◽  
Label Free ◽  
Impedance Characterization

scholarly journals Comprehensive label-free characterization of extracellular vesicles and their surface proteins

2020 ◽  
Author(s):  
E. Priglinger ◽  
J. Strasser ◽  
B. Buchroithner ◽  
F. Weber ◽  
S. Wolbank ◽  
...  
Keyword(s):  
Quality Control ◽  
Extracellular Vesicles ◽  
Impedance Measurement ◽  
Surface Proteins ◽  
Label Free ◽  
Neuroprotective Effects ◽  
Characterization Methods ◽  
Minimal Sample ◽  
Flow Filtration

AbstractInterest in mesenchymal stem cell derived extracellular vesicles (MSC-EVs) as therapeutic agents has dramatically increased over the last decade. Preclinical studies show that MSC-EVs have anti-apoptotic and neuroprotective effects, boost wound healing, and improve the integration of allogeneic grafts through immunomodulation. Current approaches to the characterization and quality control of EV-based therapeutics include particle tracking techniques, Western blotting, and advanced cytometry, but standardized methods are lacking. In this study, we established and verified quartz crystal microbalance (QCM) as highly sensitive label-free immunosensing technique for characterizing clinically approved umbilical cord MSC-EVs enriched by tangential flow filtration and ultracentrifugation. Using QCM in conjunction with common characterization methods, we were able to specifically detect EVs via EV (CD9, CD63, CD81) and MSC (CD44, CD49e, CD73) markers and gauge their prevalence. Additionally, we characterized the topography and elasticity of these EVs by atomic force microscopy (AFM), enabling us to distinguish between EVs and non-vesicular particles (NVPs) in a therapeutic formulation. This measurement modality makes it possible to identify EV sub-fractions, discriminate between EVs and NVPs, and to characterize EV surface proteins, all with minimal sample preparation and using label-free measurement devices with low barriers of entry for labs looking to widen their spectrum of characterization techniques. Our combination of QCM with impedance measurement (QCM-I) and AFM measurements provides a robust multi-marker approach to the characterization of clinically approved EV formulations and opens the door to improved quality control.

Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells

2017 ◽  
pp. 347-369 ◽  
Author(s):  
Maik Herbig ◽  
Martin Kräter ◽  
Katarzyna Plak ◽  
Paul Müller ◽  
Jochen Guck ◽  
...  
Keyword(s):  
Real Time ◽  
Functional Characterization ◽  
Label Free ◽  
Deformability Cytometry

Real-time determination of carcinoembryonic antigen by using a contactless electrochemical immunosensor

2016 ◽  
Vol 8 (24) ◽  
pp. 4861-4866 ◽  
Author(s):  
Yan Jin ◽  
Hongju Mao ◽  
Qinghui Jin ◽  
Jianlong Zhao
Keyword(s):  
Detection Limit ◽  
Carcinoembryonic Antigen ◽  
Real Time ◽  
Specific Binding ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Time Interaction ◽  
Time Determination ◽  
Impedance Immunosensor

A sensitive label-free and contactless impedance immunosensor is developed for the real-time interaction investigation between a cancer biomarker carcinoembryonic antigen (CEA) and its antibody CEA 5909. The immunosensor is proved to have a specific binding process between CEA and CEA 5909 with a detection limit of 100 fg mL−1in PBS.

An Air-Supported Liquid Crystal System for Real-Time and Label-Free Characterization of Phospholipases and Their Inhibitors

2008 ◽  
Vol 18 (19) ◽  
pp. 2938-2945 ◽  
Author(s):  
Deny Hartono ◽  
Xinyan Bi ◽  
Kun-Lin Yang ◽  
Lin-Yue Lanry Yung
Keyword(s):  
Liquid Crystal ◽  
Real Time ◽  
Label Free ◽  
Crystal System ◽  
Liquid Crystal System

scholarly journals Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting

2016 ◽  
Vol 9 ◽  
pp. 23-30 ◽  
Author(s):  
Thomas Mandel Clausen ◽  
Marina Ayres Pereira ◽  
Htoo Zarni Oo ◽  
Mafalda Resende ◽  
Tobias Gustavson ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Real Time ◽  
Binding Kinetics ◽  
Cancer Tissue ◽  
Primary Cancer ◽  
Label Free ◽  
Tissue Specimens
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