Active beam shaping to optimize in vivo opto-genetic cell ablation (Conference Presentation)

Investigating the RAS can be a fishy business: interdisciplinary opportunities using Zebrafish

Clinical Science ◽

10.1042/cs20180721 ◽

2018 ◽

Vol 132 (23) ◽

pp. 2469-2481 ◽

Cited By ~ 3

Author(s):

Scott Hoffmann ◽

Linda Mullins ◽

Charlotte Buckley ◽

Sebastien Rider ◽

John Mullins

Keyword(s):

Imaging Techniques ◽

Renin Angiotensin System ◽

Angiotensin System ◽

Cell Ablation ◽

Ras Inhibition ◽

Zebrafish Pronephros ◽

Identification And Characterization ◽

Structural Simplicity

The renin–angiotensin system (RAS) is highly conserved, and components of the RAS are present in all vertebrates to some degree. Although the RAS has been studied since the discovery of renin, its biological role continues to broaden with the identification and characterization of new peptides. The evolutionarily distant zebrafish is a remarkable model for studying the kidney due to its genetic tractability and accessibility for in vivo imaging. The zebrafish pronephros is an especially useful kidney model due to its structural simplicity yet complex functionality, including capacity for glomerular and tubular filtration. Both the pronephros and mesonephros contain renin-expressing perivascular cells, which respond to RAS inhibition, making the zebrafish an excellent model for studying the RAS. This review summarizes the physiological and genetic tools currently available for studying the zebrafish kidney with regards to functionality of the RAS, using novel imaging techniques such as SPIM microscopy coupled with targeted single cell ablation and synthesis of vasoactive RAS peptides.

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Gold nanoprisms for photothermal cell ablation in vivo

Nanomedicine ◽

10.2217/nnm.14.100 ◽

2014 ◽

Vol 9 (13) ◽

pp. 1913-1922 ◽

Cited By ~ 27

Author(s):

Alfredo Ambrosone ◽

Pablo del Pino ◽

Valentina Marchesano ◽

Wolfgang J Parak ◽

Jesus M de la Fuente ◽

...

Keyword(s):

Cell Ablation ◽

Gold Nanoprisms

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Langerhans cells are negative regulators of the anti-Leishmania response

Journal of Experimental Medicine ◽

10.1084/jem.20102318 ◽

2011 ◽

Vol 208 (5) ◽

pp. 885-891 ◽

Cited By ~ 125

Author(s):

Kordula Kautz-Neu ◽

Madelon Noordegraaf ◽

Stephanie Dinges ◽

Clare L. Bennett ◽

Dominik John ◽

...

Keyword(s):

Langerhans Cells ◽

Protective Immunity ◽

Low Dose ◽

Leishmania Major ◽

Interferon Γ ◽

Cell Ablation ◽

Selective Depletion ◽

T Cell Immune Responses

Migratory skin dendritic cells (DCs) are thought to play an important role in priming T cell immune responses against Leishmania major, but DC subtypes responsible for the induction of protective immunity against this pathogen are still controversial. In this study, we analyzed the role of Langerin+ skin-derived DCs in the Leishmania model using inducible in vivo cell ablation. After physiologically relevant low-dose infection with L. major (1,000 parasites), mice depleted of all Langerin+ DCs developed significantly smaller ear lesions with decreased parasite loads and a reduced number of CD4+ Foxp3+ regulatory T cells (T reg cells) as compared with controls. This was accompanied by increased interferon γ production in lymph nodes in the absence of Langerin+ DCs. Moreover, selective depletion of Langerhans cells (LCs) demonstrated that the absence of LCs, and not Langerin+ dermal DC, was responsible for the reduced T reg cell immigration and the enhanced Th1 response, resulting in attenuated disease. Our data reveal a unique and novel suppressive role for epidermal LCs in L. major infection by driving the expansion of T reg cells. A better understanding of the various roles of different DC subsets in cutaneous leishmaniasis will improve the development of a potent therapeutic/prophylactic vaccine.

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Sertoli cell ablation and replacement of the spermatogonial niche in mouse

Nature Communications ◽

10.1038/s41467-019-13879-8 ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 5

Author(s):

Tetsuhiro Yokonishi ◽

Jennifer McKey ◽

Shintaro Ide ◽

Blanche Capel

Keyword(s):

Sertoli Cells ◽

Cell Types ◽

Supporting Cell ◽

Idiopathic Male Infertility ◽

Testicular Cells ◽

Cell Ablation ◽

Toxic Drug ◽

Donor Cells ◽

Multiple Cell

AbstractSpermatogonia, which produce sperm throughout the male lifetime, are regulated inside a niche composed of Sertoli cells, and other testis cell types. Defects in Sertoli cells often lead to infertility, but replacement of defective cells has been limited by the inability to deplete the existing population. Here, we use an FDA-approved non-toxic drug, benzalkonium chloride (BC), to deplete testis cell types in vivo. Four days after BC administration, Sertoli cells are preferentially depleted, and can be replaced to promote spermatogenesis from surviving (host) spermatogonia. Seven days after BC treatment, multiple cell types can be engrafted from fresh or cryopreserved testicular cells, leading to complete spermatogenesis from donor cells. These methods will be valuable for investigation of niche-supporting cell interactions, have the potential to lead to a therapy for idiopathic male infertility in the clinic, and could open the door to production of sperm from other species in the mouse.

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A novel in vivo inducible dendritic cell ablation model in mice

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2010.05.157 ◽

2010 ◽

Vol 397 (3) ◽

pp. 559-563 ◽

Cited By ~ 6

Author(s):

Megumi Okuyama ◽

Hisako Kayama ◽

Koji Atarashi ◽

Hiroyuki Saiga ◽

Taishi Kimura ◽

...

Keyword(s):

Dendritic Cell ◽

Cell Ablation ◽

Ablation Model

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Performance analysis of the beam shaping method on optical auditory neural stimulation in vivo

Lasers in Medical Science ◽

10.1007/s10103-015-1763-0 ◽

2015 ◽

Vol 30 (5) ◽

pp. 1533-1540 ◽

Cited By ~ 2

Author(s):

Jingxuan Wang ◽

Ming Xia ◽

Jianren Lu ◽

Chen Li ◽

Xu Tian ◽

...

Keyword(s):

Performance Analysis ◽

Beam Shaping ◽

Neural Stimulation

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Vestibular and Auditory Hair Cell Regeneration Following Targeted Ablation of Hair Cells With Diphtheria Toxin in Zebrafish

Frontiers in Cellular Neuroscience ◽

10.3389/fncel.2021.721950 ◽

2021 ◽

Vol 15 ◽

Author(s):

Erin Jimenez ◽

Claire C. Slevin ◽

Luis Colón-Cruz ◽

Shawn M. Burgess

Keyword(s):

Hair Cell ◽

Inner Ear ◽

Lateral Line ◽

Hair Cells ◽

Diphtheria Toxin ◽

Adult Zebrafish ◽

Hair Cell Regeneration ◽

Cell Ablation ◽

Aquatic Vertebrates

Millions of Americans experience hearing or balance disorders due to loss of hair cells in the inner ear. The hair cells are mechanosensory receptors used in the auditory and vestibular organs of all vertebrates as well as the lateral line systems of aquatic vertebrates. In zebrafish and other non-mammalian vertebrates, hair cells turnover during homeostasis and regenerate completely after being destroyed or damaged by acoustic or chemical exposure. However, in mammals, destroying or damaging hair cells results in permanent impairments to hearing or balance. We sought an improved method for studying hair cell damage and regeneration in adult aquatic vertebrates by generating a transgenic zebrafish with the capacity for targeted and inducible hair cell ablation in vivo. This model expresses the human diphtheria toxin receptor (hDTR) gene under the control of the myo6b promoter, resulting in hDTR expressed only in hair cells. Cell ablation is achieved by an intraperitoneal injection of diphtheria toxin (DT) in adult zebrafish or DT dissolved in the water for larvae. In the lateral line of 5 days post fertilization (dpf) zebrafish, ablation of hair cells by DT treatment occurred within 2 days in a dose-dependent manner. Similarly, in adult utricles and saccules, a single intraperitoneal injection of 0.05 ng DT caused complete loss of hair cells in the utricle and saccule by 5 days post-injection. Full hair cell regeneration was observed for the lateral line and the inner ear tissues. This study introduces a new method for efficient conditional hair cell ablation in adult zebrafish inner ear sensory epithelia (utricles and saccules) and demonstrates that zebrafish hair cells will regenerate in vivo after this treatment.

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Time-dependent Photodynamic Therapy for Multiple Targets: A Highly Efficient AIE-active Photosensitizer for Selective Bacterial Elimination and Cancer Cell Ablation

10.26434/chemrxiv.9829442.v1 ◽

2019 ◽

Author(s):

Qiyao Li ◽

Ying Li ◽

Tianliang Min ◽

Junyi Gong ◽

Lili Du ◽

...

Keyword(s):

Photodynamic Therapy ◽

Reactive Oxygen Species Generation ◽

Emission Feature ◽

Time Dependent ◽

Organic Salt ◽

Antibacterial Performance ◽

Cell Ablation ◽

External Conditions

Pathogen infection and cancer are the two major human health problems. In this work, we achieved an organic salt photosensitizer (PS), called 4TPA-BQ with aggregation-induced emission feature via one-step reaction. Owing to the aggregation-induced reactive oxygen species generation effect and sufficient small ΔEST, 4TPA-BQ shows a satisfactorily high 1O2 generation efficiency of 97.8%. In vitro and in vivo experiments confirmed that 4TPA-BQ exhibited potent photodynamic antibacterial performance against ampicillin-resistant Escherichia coli with good biocompatibility in a short time (15 min). When the incubation time persisted long enough (12 h), cancer cells were ablated efficiently, leaving normal cells essentially unaffected. This is the first reported time-dependent fluorescence-guided photodynamic therapy in one individual PS for ordered and multiple targeting by varying the external conditions. This can update the design principle of efficient PSs in potential clinical applications.

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