In-line balanced detection stimulated Raman scattering microscopy from a compact fiber-format laser source (Conference Presentation)

2018 ◽  
Author(s):  
Dario Polli ◽  
Francesco Crisafi ◽  
Vikas Kumar ◽  
Giulio Cerullo
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Laser Source ◽  
Balanced Detection ◽  
Stimulated Raman

Virtual brain tumor histopathology and immunohistochemistry with stimulated raman scattering microscopy.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e13511-e13511
Author(s):  
Spencer B Lewis ◽  
Balaji Pandian ◽  
Todd Hollon ◽  
Yashar Niknafs ◽  
Mia Garrard ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Frozen Section ◽  
Ease Of Use ◽  
Laser Source ◽  
Raman Signal ◽  
Wild Type ◽  
Spectral Difference ◽  
Stimulated Raman

e13511 Background: Stimulated Raman scattering (SRS) microscopy is a label-free optical technique which uses the chemical composition of fresh tissue to generate image contrast. Prior work has demonstrated exceptional correlation between SRS images and H&E microscopy for detecting brain tumor infiltration ex vivo. However, SRS has not previously been suitable for a clinical environment. Further, SRS has utilized structural, rather than chemical, diagnostic features. We present the first clinical validation of SRS, as well as our work towards mutation-targeted SRS imaging. Methods: Implementation of SRS with a fiber laser source allowed sufficient stability for clinical deployment. Unprocessed specimens from 200 neurosurgical cases were imaged at the CH2 and CH3 vibrational modes, then processed to mimic the appearance of H&E staining. 30 cases were trialed in a simulated intraoperative consultation to assess concordance between SRS and cryosection microscopy. A multi-layer perceptron (MLP) model was then used to predict brain tumor diagnosis based on quantified image attributes. To explore the potential for chemically specific SRS, isogenic lines of normal human astrocyte were prepared, differing by the presence or absence of the R132H mutation in the isocitrate dehydrogenase 1 (IDH1) gene. IDH1 R132H cells accumulate high concentrations of the oncometabolite 2-hydroxyglutarate and display predictably altered metabolism. 200 Raman spectra were collected from 50 wild type (WT) and 50 R132H cells with the aim of detecting these changes. Each cell line was then submitted for targeted metabolomic analysis. Results: Excellent concordance was observed between SRS and frozen section histology ( κ >0.89). The MLP diagnosed brain tumor subtype with 90% accuracy. A robust spectral difference was observed between wild type and R132H cells, which showed concordance with metabolomic data. Conclusions: The diagnostic capability, ease of use and speed of SRS make it well-suited for integration into the operative workflow. Further, because SRS amplifies the Raman signal with no resonant background, the spectral differences observed may allow rapid differentiation of IDH1 WT and R132H cells with SRS.

scholarly journals Simultaneous Second-Harmonic, Sum-Frequency Generation and Stimulated Raman Scattering in MgO:PPLN

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2266
Author(s):  
Dismas Choge ◽  
Huaixi Chen ◽  
Lei Guo ◽  
Guangwei Li ◽  
Wanguo Liang
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Second Harmonic ◽  
Sum Frequency Generation ◽  
Laser Source ◽  
Sum Frequency ◽  
Wide Range ◽  
Multi Wavelength ◽  
Frequency Generation ◽  
Stimulated Raman

In this study, simultaneous second-harmonic generation (SHG), sum frequency generation (SFG), and Raman conversion based on MgO-doped periodically poled lithium niobate (MgO:PPLN) for multi-wavelength generation is demonstrated. The approach used is based on a single MgO:PPLN crystal poled with a uniform period of 10.2 µm that phase matches SHG and SFG, simultaneously. Using a simplified double-pass geometry, up to 0.8 W of blue light at 487 nm is achieved by a frequency-doubling 974 nm laser diode pump, and 0.5 W of orange light at 598 nm is generated by frequency mixing 974 nm pump with C-band (1527–1565 nm) tunable laser source. At high pump powers of the 974 nm laser source, other unexpected peaks at 437, 536, 756, 815 and 1038 nm were observed, of which the 1038 nm line is due to Stimulated Raman Scattering within the MgO:PPLN crystal. The resulting multi-wavelength light source may find a wide range of applications in biomedicine and basic research.

scholarly journals Stimulated Raman scattering microscopy with a robust fibre laser source

2014 ◽  
Vol 8 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Christian W. Freudiger ◽  
Wenlong Yang ◽  
Gary R. Holtom ◽  
Nasser Peyghambarian ◽  
X. Sunney Xie ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Laser Source ◽  
Fibre Laser ◽  
Stimulated Raman

Research on 1.7 μm Fiber Laser Source Based on Stimulated Raman Scattering of Hydrogen in Hollow-core Fiber

2020 ◽  
Vol 40 (5) ◽  
pp. 0514001
Author(s):  
黄威 Huang Wei ◽  
崔宇龙 Cui Yulong ◽  
李智贤 Li Zhixian ◽  
周智越 Zhou Zhiyue ◽  
王泽锋 Wang Zefeng
Keyword(s):  
Raman Scattering ◽  
Fiber Laser ◽  
Stimulated Raman Scattering ◽  
Laser Source ◽  
Hollow Core ◽  
Core Fiber ◽  
Stimulated Raman

scholarly journals In-line balanced detection stimulated Raman scattering microscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Francesco Crisafi ◽  
Vikas Kumar ◽  
Tullio Scopigno ◽  
Marco Marangoni ◽  
Giulio Cerullo ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Balanced Detection ◽  
Stimulated Raman

Shot-Noise-Limited Two-Color Stimulated Raman Scattering Microscopy with a Balanced Detection Scheme

2020 ◽  
Vol 124 (13) ◽  
pp. 2591-2599
Author(s):  
Youngjin Choi ◽  
Sohee Lim ◽  
Joong Won Shim ◽  
Bonghwan Chon ◽  
Jong Min Lim ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Shot Noise ◽  
Stimulated Raman Scattering ◽  
Detection Scheme ◽  
Balanced Detection ◽  
Stimulated Raman
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