Femtosecond-laser setup for cell-membrane poration

2018 ◽  
Author(s):  
Karsten König ◽  
Hans G. Breunig ◽  
Ana Batista ◽  
Benjamin Sauer ◽  
Aisada König
Keyword(s):  
Cell Membrane ◽  
Femtosecond Laser ◽  
Membrane Poration ◽  
Laser Setup

Evidence of femtosecond-laser pulse induced cell membrane nanosurgery

2017 ◽  
Author(s):  
Nir Katchinskiy ◽  
Roseline Godbout ◽  
Abdulhakem Y. Elezzabi
Keyword(s):  
Cell Membrane ◽  
Laser Pulse ◽  
Femtosecond Laser ◽  
Femtosecond Laser Pulse

Visualization of Cell Membrane Poration by Microbubble Oscillation

2015 ◽  
pp. 897-898
Author(s):  
S. Moosavi-Nejad ◽  
K. Tachibana ◽  
H. Akiyama ◽  
S. H. R. Hosseini
Keyword(s):  
Cell Membrane ◽  
Membrane Poration

scholarly journals Characterization of femtosecond-laser pulse induced cell membrane nanosurgical attachment

2016 ◽  
Vol 7 (7) ◽  
pp. 2749 ◽  
Author(s):  
Nir Katchinskiy ◽  
Roseline Godbout ◽  
Abdulhakem Y. Elezzabi
Keyword(s):  
Cell Membrane ◽  
Laser Pulse ◽  
Femtosecond Laser ◽  
Femtosecond Laser Pulse

scholarly journals Cell membrane deformation and bioeffects produced by tandem bubble-induced jetting flow

2015 ◽  
Vol 112 (51) ◽  
pp. E7039-E7047 ◽  
Author(s):  
Fang Yuan ◽  
Chen Yang ◽  
Pei Zhong
Keyword(s):  
Cell Membrane ◽  
Strain Rate ◽  
Cell Survival ◽  
Bubble Dynamics ◽  
Leading Edge ◽  
Experimental System ◽  
Maximum Diameter ◽  
Membrane Deformation ◽  
Membrane Poration ◽  
Area Strain

Cavitation with bubble–bubble interaction is a fundamental feature in therapeutic ultrasound. However, the causal relationships between bubble dynamics, associated flow motion, cell deformation, and resultant bioeffects are not well elucidated. Here, we report an experimental system for tandem bubble (TB; maximum diameter = 50 ± 2 μm) generation, jet formation, and subsequent interaction with single HeLa cells patterned on fibronectin-coated islands (32 × 32 μm) in a microfluidic chip. We have demonstrated that pinpoint membrane poration can be produced at the leading edge of the HeLa cell in standoff distance Sd ≤ 30 μm, driven by the transient shear stress associated with TB-induced jetting flow. The cell membrane deformation associated with a maximum strain rate on the order of 104 s−1 was heterogeneous. The maximum area strain (εA,M) decreased exponentially with Sd (also influenced by adhesion pattern), a feature that allows us to create distinctly different treatment outcome (i.e., necrosis, repairable poration, or nonporation) in individual cells. More importantly, our results suggest that membrane poration and cell survival are better correlated with area strain integral (∫​εA2dt) instead of εA,M, which is characteristic of the response of materials under high strain-rate loadings. For 50% cell survival the corresponding area strain integral was found to vary in the range of 56 ∼ 123 μs with εA,M in the range of 57 ∼ 87%. Finally, significant variations in individual cell’s response were observed at the same Sd, indicating the potential for using this method to probe mechanotransduction at the single cell level.

Noncontact microsurgery of living cell membrane using femtosecond laser pulses

2013 ◽  
Author(s):  
I. V. Ilina ◽  
A. V. Ovchinnikov ◽  
D. S. Sitnikov ◽  
O. V. Chefonov ◽  
M. B. Agranat ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Femtosecond Laser ◽  
Living Cell ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses

scholarly journals Advanced nanostructures for cell membrane poration

2019 ◽  
Vol 30 (26) ◽  
pp. 264002 ◽  
Author(s):  
Apresio K Fajrial ◽  
Xiaoyun Ding
Keyword(s):  
Cell Membrane ◽  
Membrane Poration

scholarly journals Tumour Cell Membrane Poration and Ablation by Pulsed Low-Intensity Electric Field with Carbon Nanotubes

2015 ◽  
Vol 16 (12) ◽  
pp. 6890-6901 ◽  
Author(s):  
Lijun Wang ◽  
Dun Liu ◽  
Ru Zhou ◽  
Zhigang Wang ◽  
Alfred Cuschieri
Keyword(s):  
Carbon Nanotubes ◽  
Cell Membrane ◽  
Electric Field ◽  
Tumour Cell ◽  
Low Intensity ◽  
Membrane Poration

Electrical Analysis of Cell Membrane Poration by an Intense Nanosecond Pulsed Electric Field Using an Atomistic-to-Continuum Method

2015 ◽  
Vol 63 (6) ◽  
pp. 2032-2040 ◽  
Author(s):  
Sophie Kohler ◽  
Zachary A. Levine ◽  
Miguel A. Garcia-Fernandez ◽  
Ming-Chak Ho ◽  
P. Thomas Vernier ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Electric Field ◽  
Pulsed Electric Field ◽  
Nanosecond Pulsed Electric Field ◽  
Electrical Analysis ◽  
Membrane Poration
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