Fluorescence visualization of wound closure in partial and full-thickness wound models (Conference Presentation)

2018 ◽  
Author(s):  
Ying Wang ◽  
Antonio Ortega-Martinez ◽  
William A. Farinelli ◽  
Richard R. Anderson ◽  
Walfre Franco
Keyword(s):  
Wound Closure ◽  
Full Thickness ◽  
Full Thickness Wound

One‐step Synthesis of Multifunctional Chitosan Hydrogel for Full‐thickness Wound Closure and Healing

2021 ◽  
pp. 2101808
Author(s):  
Shen Guo ◽  
Minghao Yao ◽  
Dan Zhang ◽  
Yuanmeng He ◽  
Rong Chang ◽  
...  
Keyword(s):  
Wound Closure ◽  
Full Thickness ◽  
Chitosan Hydrogel ◽  
One Step ◽  
Full Thickness Wound

scholarly journals Vital, Porcine, Gal-Knockout Skin Transplants Provide Efficacious Temporary Closure of Full-Thickness Wounds: Good Laboratory Practice-Compliant Studies in Nonhuman Primates

2020 ◽  
Vol 41 (2) ◽  
pp. 229-240 ◽  
Author(s):  
Paul Holzer ◽  
Jon Adkins ◽  
Krysta Moulton ◽  
Laurence Zhu ◽  
Rod Monroy ◽  
...  
Keyword(s):  
Wound Closure ◽  
Preclinical Study ◽  
Genetically Engineered ◽  
Great Promise ◽  
Full Thickness ◽  
Current Standard ◽  
Miniature Swine ◽  
Burn Wounds ◽  
Temporary Closure ◽  
Full Thickness Wound

Abstract Vital, genetically engineered porcine skin transplants have long been regarded as a promising treatment option for severe burn wounds. The objective of this two-part, preclinical study was to evaluate the ability of vital, split-thickness skin xenotransplants derived from designated pathogen-free, alpha 1,3 galactosyltransferase knockout miniature swine to provide temporary wound closure of full-thickness wound defects intended to model severe and extensive, deep partial- and full-thickness burn wounds. In part 1 of the study, four full-thickness wound defects were introduced in four cynomolgus macaques recipients and, then engrafted with two xenografts and two allografts to achieve temporary wound closure. On POD-15, autografts were used to achieve definitive wound closure and were observed until POD-22. In part 2 of the study, four additional subjects each received two full-thickness wound defects, followed by two xenografts to achieve temporary wound closure, and were observed postoperatively for 30 days without further intervention. All grafts were assessed for signs of adherence to the wound bed, vascularity, and signs of immune rejection via gross clinical and histological methods. Xenograft and allograft comparators were equivalent in part 1, and later autografts were otherwise indistinguishable. In part 2, all xenotransplants demonstrated adherence, vascularity, and survival until POD-30. These were unexpected results that exceed previously published findings in similar models. Furthermore, the ensuing GLP-study report directly supported regulatory clearance, permitting a phase I clinical trial. This solution holds great promise as an alternative to human cadaver allograft, the current standard of care for the treatment of severe burns.

scholarly journals Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 854
Author(s):  
Ahmad Hivechi ◽  
Peiman Brouki Milan ◽  
Khashayar Modabberi ◽  
Moein Amoupour ◽  
Kaveh Ebrahimzadeh ◽  
...  
Keyword(s):  
Cell Activity ◽  
Average Diameter ◽  
Skin Substitute ◽  
Full Thickness ◽  
Skin Integrity ◽  
Hematoxylin And Eosin ◽  
First Time ◽  
Full Thickness Wound

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats’ full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson’s Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

Generation of Nano-pores in Silk Fibroin Films Using Silk Nanoparticles for Full-Thickness Wound Healing

2021 ◽  
Vol 22 (2) ◽  
pp. 546-556
Author(s):  
Jian Liu ◽  
Ran Huang ◽  
Gang Li ◽  
David L. Kaplan ◽  
Zhaozhu Zheng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Full Thickness ◽  
Full Thickness Wound

A multifunctional substance P-conjugated chitosan hydrochloride hydrogel accelerates full-thickness wound healing by enhancing synchronized vascularization, extracellular matrix deposition, and nerve regeneration

2021 ◽  
Author(s):  
Hao Li ◽  
Mengna Li ◽  
Pei Liu ◽  
Kai-Yang Wang ◽  
Haoyu Fang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Full Thickness ◽  
Matrix Deposition ◽  
Chitosan Hydrochloride ◽  
Reconstructive Microsurgery ◽  
Extracellular Matrix Deposition ◽  
Advanced Biomaterials ◽  
Native Skin ◽  
Full Thickness Wound

Due to the native skin limitations and the complexity of reconstructive microsurgery, advanced biomaterials are urgently required to promote wound healing for severe skin defects caused by accidents and disasters....

Bioactive antibacterial bilayer PCL/gelatin nanofibrous scaffold promotes full-thickness wound healing

2020 ◽  
Vol 583 ◽  
pp. 119413 ◽  
Author(s):  
Arman Jafari ◽  
Armin Amirsadeghi ◽  
Shadi Hassanajili ◽  
Negar Azarpira
Keyword(s):  
Wound Healing ◽  
Nanofibrous Scaffold ◽  
Full Thickness ◽  
Full Thickness Wound

Comparative evaluation of surface swab and quantitative full thickness wound biopsy culture in burn patients

Burns ◽  
2007 ◽  
Vol 33 (4) ◽  
pp. 460-463 ◽  
Author(s):  
Sanjeev K. Uppal ◽  
Shobha Ram ◽  
Bharti Kwatra ◽  
Sumit Garg ◽  
R. Gupta
Keyword(s):  
Comparative Evaluation ◽  
Burn Patients ◽  
Full Thickness ◽  
Full Thickness Wound
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