Light scattering in biological tissues: comparison between theory and experiments of spatial and temporal intensity profiles

1995 ◽  
Author(s):  
Frederic P. Bevilacqua ◽  
Pierre Marquet ◽  
Christian D. Depeursinge
Keyword(s):  
Light Scattering ◽  
Biological Tissues ◽  
Theory And Experiments ◽  
Temporal Intensity

Enhancement of OCT imaging by blood optical clearing in vessels – A feasibility study

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Olga Zhernovaya ◽  
Valery V. Tuchin ◽  
Martin J. Leahy
Keyword(s):  
Light Scattering ◽  
Blood Vessels ◽  
Feasibility Study ◽  
Biological Tissues ◽  
Intradermal Injection ◽  
Light Transport ◽  
Optical Clearing ◽  
Intravenous Injections ◽  
Oct Imaging ◽  
System Operating

AbstractThe results of a feasibility study of the application of PEG-300 and fructose as two independent optical clearing agents for the reduction of light scattering in biological tissues are presented.An OCT system operating at 1300 nm was used to study optical clearing effects. InThe intradermal injection of fructose in combination with the intravenous injection of PEG-300 led to a rapid optical clearing effect. In the experiments on miceThe experiments on mice have clearly demonstrated that intradermal and intravenous injections of optical clearing agents enhanced light transport through the skin and blood vessels.

scholarly journals Perspective: Wavefront shaping techniques for controlling multiple light scattering in biological tissues: Towardin vivoapplications

APL Photonics ◽  
2018 ◽  
Vol 3 (10) ◽  
pp. 100901 ◽  
Author(s):  
Jung-Hoon Park ◽  
Zhipeng Yu ◽  
KyeoReh Lee ◽  
Puxiang Lai ◽  
YongKeun Park
Keyword(s):  
Light Scattering ◽  
Biological Tissues ◽  
Wavefront Shaping ◽  
Multiple Light Scattering

scholarly journals Protein-mediated gelation and nano-scale assembly of unfunctionalized hyaluronic acid and chondroitin sulfate

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1827
Author(s):  
Anthony Tabet ◽  
June Y. Park ◽  
Jarrod Shilts ◽  
Kamil Sokolowski ◽  
Vijay K. Rana ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Nervous System ◽  
Hyaluronic Acid ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Chondroitin Sulfate ◽  
Biological Tissues ◽  
Simple Method ◽  
Vis Spectroscopy

Background: Hyaluronic acid (HA) is a major component of the extracellular matrix (ECM) in the central nervous system and the only purely supramolecular glycosaminoglycan. Much focus has been given to using this high molecular weight polysaccharide for tissue engineering applications. In most studies, the backbone of HA is functionalized with moieties that can facilitate network formation through physical self-assembly, or covalent crosslinking (e.g. photo-catalyzed) at concentrations where the polysaccharide does not gel on its own. However, these crosslinks often utilize functional groups not found in biological tissues. Methods: Oscillatory rheology, dynamic light scattering, and scanning electron microscopy were used to study albumin/HA structures. Dynamic light scattering and transmission electron microscopy were used to study albumin/chondroitin sulfate (CS) structures. UV-vis spectroscopy was used to demonstrate the potential for using protein-polymer blends as an ECM-mimetic model to study transport of small molecules. Results: We examine the intermolecular interactions of two major glycosaminoglycans found in the human brain, HA and the lower molecular weight CS, with the model protein albumin. We report the properties of the resulting micro- and nano materials. Our albumin/HA systems formed gels, and albumin/CS systems formed micro- and nanoparticles. These systems are formed from unfunctionalized polysaccharides, which is an attractive and simple method of forming HA hydrogels and CS nanoparticles. We also summarize the concentrations of HA and CS found in various mammalian brains, which could potentially be useful for biomimetic scaffold development. Conclusions: Simple preparation of commercially available charged biomacromolecules results in interesting materials with structures at the micron and nanometer length-scales. Such materials may have utility in serving as cost-effective models of nervous system electrostatic interactions and as in vitro drug release and model system for ECM transport studies.

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