Robust SERS spectral analysis for quantitative detection of pyocyanin in biological fluids

2017 ◽  
Author(s):  
William Thrift ◽  
Arunima Bhattacharjee ◽  
Allon I. Hochbaum ◽  
Regina Ragan ◽  
Cuong Nguyen ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Biological Fluids ◽  
Quantitative Detection

Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens

2019 ◽  
Vol 11 (33) ◽  
pp. 29619-29629 ◽  
Author(s):  
Yujie Tu ◽  
Yeqing Yu ◽  
Zhibiao Zhou ◽  
Sheng Xie ◽  
Bicheng Yao ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Water Soluble ◽  
Quantitative Detection

scholarly journals Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies

2018 ◽  
Vol 115 (5) ◽  
pp. E925-E933 ◽  
Author(s):  
Roxana Jalili ◽  
Joe Horecka ◽  
James R. Swartz ◽  
Ronald W. Davis ◽  
Henrik H. J. Persson
Keyword(s):  
Limit Of Detection ◽  
Biological Fluids ◽  
Protein Detection ◽  
Ease Of Use ◽  
Proximity Ligation Assay ◽  
Quantitative Detection ◽  
High Signal ◽  
Affinity Reagents ◽  
Proximity Ligation ◽  
Assay Format

Proximity ligation assay (PLA) is a powerful tool for quantitative detection of protein biomarkers in biological fluids and tissues. Here, we present the circular proximity ligation assay (c-PLA), a highly specific protein detection method that outperforms traditional PLA in stringency, ease of use, and compatibility with low-affinity reagents. In c-PLA, two proximity probes bind to an analyte, providing a scaffolding that positions two free oligonucleotides such that they can be ligated into a circular DNA molecule. This assay format stabilizes antigen proximity probe complexes and enhances stringency by reducing the probability of random background ligation events. Circle formation also increases selectivity, since the uncircularized DNA can be removed enzymatically. We compare this method with traditional PLA on several biomarkers and show that the higher stringency for c-PLA improves reproducibility and enhances sensitivity in both buffer and human plasma. The limit of detection ranges from femtomolar to nanomolar concentrations for both methods. Kinetic analyses using surface plasmon resonance (SPR) and biolayer interferometry (BLI) reveal that the variation in limit of detection is due to the variation in antibody affinity and that c-PLA outperforms traditional PLA for low-affinity antibodies. The lower background signal can be used to increase proximity probe concentration while maintaining a high signal-to-noise ratio, thereby enabling the use of low-affinity reagents in a homogeneous assay format. We anticipate that the advantages of c-PLA will be useful in a variety of clinical protein detection applications where high-affinity reagents are lacking.

Quantitative Detection of PEGylated Biomacromolecules in Biological Fluids by NMR

2016 ◽  
Vol 88 (7) ◽  
pp. 3730-3738 ◽  
Author(s):  
Rohan D. A. Alvares ◽  
Advait Hasabnis ◽  
R. Scott Prosser ◽  
Peter M. Macdonald
Keyword(s):  
Biological Fluids ◽  
Quantitative Detection

scholarly journals Determination of eugenitin in mouse blood by ultra-performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study

2021 ◽  
Author(s):  
Chongliang Lin ◽  
Dezhen Song ◽  
Haodong Jiang ◽  
Lvqi Luo ◽  
Xi Bao ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Ultra Performance Liquid Chromatography ◽  
Quantitative Detection ◽  
Syzygium Aromaticum ◽  
Mouse Blood ◽  
The Matrix ◽  
Liquid Chromatography Tandem Mass

Abstract Eugenitin is a non-volatile chromone derivative which is always found in dried flower buds of Syzygium aromaticum L. (Merr.) & L.M. Perry. Until now, there were no reports about the pharmacokinetics of eugenitin in biological fluids. A UPLC-MS/MS method developed to determine eugenitin in mouse blood. The blood samples were prepared by protein precipitation with acetonitrile. Chrysin (internal standard, IS) and eugenitin were gradient eluted by mobile phase of acetonitrile and water (0.1% formic acid) in a BEH C18 column. The multiple reaction monitoring (MRM) of m/z 221.1→206.0 for eugenitin and m/z 255.1→152.9 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 500 ng/mL (r > 0.995). The accuracy ranged from 98 to 113%, the precision was less than 12%, and the matrix effect was between 86 and 94%, the recovery was better than 81%. The developed method was successfully used for pharmacokinetics of eugenitin in mice after intravenous (5 mg/kg) and oral (20 mg/kg) administration, and the absolute availability of eugenitin was 12%.

scholarly journals A Paper-Based Near-Infrared Optical Biosensor for Quantitative Detection of Protease Activity Using Peptide-Encapsulated SWCNTs

Sensors ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 5247 ◽  
Author(s):  
Vlad Shumeiko ◽  
Yossi Paltiel ◽  
Gili Bisker ◽  
Zvi Hayouka ◽  
Oded Shoseyov
Keyword(s):  
Protease Activity ◽  
Near Infrared ◽  
Biological Fluids ◽  
Point Of Care ◽  
Optical Biosensor ◽  
Infrared Region ◽  
Enzymatic Digestion ◽  
Single Wall Carbon Nanotubes ◽  
Quantitative Detection ◽  
Significant Drop

A protease is an enzyme that catalyzes proteolysis of proteins into smaller polypeptides or single amino acids. As crucial elements in many biological processes, proteases have been shown to be informative biomarkers for several pathological conditions in humans, animals, and plants. Therefore, fast, reliable, and cost-effective protease biosensors suitable for point-of-care (POC) sensing may aid in diagnostics, treatment, and drug discovery for various diseases. This work presents an affordable and simple paper-based dipstick biosensor that utilizes peptide-encapsulated single-wall carbon nanotubes (SWCNTs) for protease detection. Upon enzymatic digestion of the peptide, a significant drop in the photoluminescence (PL) of the SWCNTs was detected. As the emitted PL is in the near-infrared region, the developed biosensor has a good signal to noise ratio in biological fluids. One of the diseases associated with abnormal protease activity is pancreatitis. In acute pancreatitis, trypsin concentration could reach up to 84 µg/mL in the urine. For proof of concept, we demonstrate the feasibility of the proposed biosensor for the detection of the abnormal levels of trypsin activity in urine samples.

scholarly journals New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5514
Author(s):  
Clara Abardía-Serrano ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Prostate Cancer ◽  
Cancer Screening ◽  
Nucleic Acid ◽  
Magnetic Beads ◽  
Dynamic Range ◽  
Prostate Cancer Screening ◽  
Biological Fluids ◽  
Quantitative Detection ◽  
Personal Glucose Meter ◽  
Glucose Meter

A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

From “Clinical Proteomics” to “Clinical Chemistry Proteomics”: considerations using quantitative mass-spectrometry as a model approach

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Sylvain Lehmann ◽  
Pauline Poinot ◽  
Laurent Tiers ◽  
Christophe Junot ◽  
François Becher ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Discovery ◽  
Clinical Chemistry ◽  
Biological Fluids ◽  
Clinical Proteomics ◽  
Detection Methods ◽  
Patient Treatment ◽  
Quantitative Detection ◽  
New Biomarkers

AbstractClinical Proteomics biomarker discovery programs lead to the selection of putative new biomarkers of human pathologies. Following an initial discovery phase, validation of these candidates in larger populations is a major task that recently started relying upon the use of mass spectrometry approaches, especially in cases where classical immune-detection methods were lacking. Thanks to highly sensitive spectrometers, adapted measurement methods like selective reaction monitoring (SRM) and various pre-fractionation methods, the quantitative detection of protein/peptide biomarkers in low concentrations is now feasible from complex biological fluids. This possibility leads to the use of similar methodologies in clinical biology laboratories, within a new proteomic field that we shall name “Clinical Chemistry Proteomics” (CCP). Such evolution of Clinical Proteomics adds important constraints with regards to the in vitro diagnostic (IVD) application. As measured values of analytes will be used to diagnose, follow-up and adapt patient treatment on a routine basis; medical utility, robustness, reference materials and clinical feasibility are among the new issues of CCP to consider.

scholarly journals Giant magnetoresistive biosensors for real-time quantitative detection of protease activity

2020 ◽  
Author(s):  
Sandeep Adem ◽  
Sonal Jain ◽  
Michael Sveiven ◽  
Xiahan Zhou ◽  
Anthony J. O’Donoghue ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Real Time ◽  
Protease Activity ◽  
Biological Fluids ◽  
Time Dependent ◽  
Local Magnetic Field ◽  
Quantitative Detection ◽  
Healthy Human ◽  
Dependent Change ◽  
Time Dependent Change

ABSTRACTProteases are enzymes that cleave proteins and are crucial to physiological processes such as digestion, blood clotting, and wound healing. Unregulated protease activity is a biomarker of several human diseases. Synthetic peptides that are selectively hydrolyzed by a protease of interest can be used as reporter substrates of unregulated protease activity. We developed an activity-based protease sensor by immobilizing magnetic nanoparticles (MNP) to the surface of a giant magnetoresistive spin-valve (GMR SV) sensor using peptides. Cleavage of these peptides by a protease, releases the magnetic nanoparticles resulting in a time-dependent change in the local magnetic field. Using this approach, we detected a significant release of MNPs after 3.5 minutes incubation using just 4 nM of the cysteine protease, papain. In addition, we show that proteases in healthy human urine do not release the MNPs, however addition of 20 nM of papain to the urine samples resulted in a time-dependent change in magnetoresistance. This study lays the foundation for using GMR SV sensors as a platform for real-time quantitative detection of protease activity in biological fluids.

Spectral Analysis and Quantitative Detection of Baicalin Based on Terahertz Radiation

2020 ◽  
Vol 47 (3) ◽  
pp. 0314001
Author(s):  
刘陵玉 Liu Lingyu ◽  
常天英 Chang Tianying ◽  
李珂 Li Ke ◽  
李羿璋 Li Yizhang ◽  
张献生 Zhang Xiansheng ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Terahertz Radiation ◽  
Quantitative Detection
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