scholarly journals Study of inertial hydrodynamic focusing in sheath-driven flows for lab-on-a-chip flow cytometry

2017 ◽  
Author(s):  
Nishtha Panwar ◽  
Peiyi Song ◽  
Ken-Tye Yong ◽  
Swee Chuan Tjin
Keyword(s):  
Flow Cytometry ◽  
Lab On A Chip ◽  
Hydrodynamic Focusing ◽  
Chip Flow

Multiparameter Lab-on-a-Chip flow cytometry of the cellcycle

2013 ◽  
Vol 42 ◽  
pp. 586-591 ◽  
Author(s):  
Joanna Skommer ◽  
Jin Akagi ◽  
Kazuo Takeda ◽  
Yuu Fujimura ◽  
Khashayar Khoshmanesh ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Lab On A Chip ◽  
Chip Flow

Multiparameter Analysis of Apoptosis Using Lab‐on‐a‐Chip Flow Cytometry

2013 ◽  
Vol 66 (1) ◽  
Author(s):  
Donald Wlodkowic ◽  
Joanna Skommer ◽  
Jin Akagi ◽  
Yoo Fujimura ◽  
Kazuo Takeda
Keyword(s):  
Flow Cytometry ◽  
Lab On A Chip ◽  
Chip Flow ◽  
Multiparameter Analysis

scholarly journals 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haoran Wang ◽  
Anton Enders ◽  
John-Alexander Preuss ◽  
Janina Bahnemann ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Single Cells ◽  
Lab On A Chip ◽  
Cost Effective ◽  
Optical Manipulation ◽  
Hydrodynamic Focusing ◽  
Trapping Region ◽  
Dual Beam ◽  
3D Printed ◽  
On Chip

Abstract3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.

The Future of Flow Cytometry in Biotechnology: The Response to Diversity and Complexity

2005 ◽  
Author(s):  
Larry A. Sklar
Keyword(s):  
Flow Cytometry ◽  
Scattered Light ◽  
National Laboratory ◽  
Modern World ◽  
Light Sources ◽  
Main Function ◽  
Hydrodynamic Focusing ◽  
Los Alamos National Laboratory ◽  
The Future ◽  
Dependent Cell

Flow cytometry is a mature technology: Instruments recognizable as having elements of modern flow cytometers date back at least 30 years. There are many good sources for information about the essential features of flow cytometers, how they operate, and how they have been used. For the purposes of this book, it is necessary to know that flow cytometers have fluidic, optical, electronic, computational, and mechanical features. The main function of the fluidic components is to use hydrodynamic focusing to create a stable particle stream in which particles are aligned in single file within a sheath stream, so that the particles can be analyzed and sorted. The main functions of the optical components are to allow the particles to be illuminated by one or more lasers or other light sources and to allow scattered light as well as multiple fluorescence signals to be resolved and be routed to individual detectors. The electronics coordinate these functions, from the acquisition of the signals (pulse collection, pulse analysis, triggering, time delay, data, gating, detector control) to forming and charging individual droplets, and to making sort decisions. The computational components are directed at postacquisition data display and analysis, analysis of multivariate populations and multiplexing assays, and calibration and analysis of time-dependent cell or reaction phenomena. Mechanical components are now being integrated with flow cytometers to handle plates of samples and to coordinate automation such as the movement of a cloning tray with the collection of the droplets. The reader is directed to a concise description of these processes in Robinson’s article in the Encyclopedia of Biomaterials and Biomedical Engineering. This book was conceived of to provide a perspective on the future of flow cytometry, and particularly its application to biotechnology. It attempts to answer the question I heard repeatedly, especially during my association with the National Institutes of Health–funded National Flow Cytometry Resource at Los Alamos National Laboratory: What is the potential for innovation in flow cytometer design and application? This volume brings together those approaches that identify the unique contributions of flow cytometry to the modern world of biotechnology.

On-Chip Flow Cytometry

2012 ◽  
pp. 1913-1923 ◽  
Author(s):  
Shady Gawad ◽  
Ana Valero ◽  
Thomas Braschler ◽  
David Holmes ◽  
Philippe Renaud ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chip Flow ◽  
On Chip

scholarly journals Dielectrophoretic 3D‐focusing for on‐chip flow cytometry

2020 ◽  
Vol 15 (5) ◽  
pp. 296-301
Author(s):  
Salini Krishna ◽  
Fadi Alnaimat ◽  
Ali Hilal‐Alnaqbi ◽  
Saud Khashan ◽  
Bobby Mathew
Keyword(s):  
Flow Cytometry ◽  
Chip Flow ◽  
On Chip

Microcapsule Generation Using a Compound Jet Instability

2006 ◽  
Author(s):  
Sang-Youp Lee ◽  
Connie Snider ◽  
Kinam Park ◽  
J. Paul Robinson
Keyword(s):  
Flow Cytometry ◽  
Current System ◽  
Hydrodynamic Focusing ◽  
Pressure Balance ◽  
Jet Instability ◽  
Generation Technique ◽  
Component Solution ◽  
Reservoir Type

A new microcapsule generation technique is presented. The microcapsule system has been built based on conventional flow cytometry. The results show that the current system can control the jet diameters of each component solution comprising a compound jet, in addition to the resultant microcapsule diameter, using the pressure balance between adjacent fluids in the hydrodynamic focusing region. It has been also shown that the synchronized breakup of the compound jet is critical for the reservoir-type morphology of the microcapsules.

Fabrication of Microfluidic Chip and Test of a Hydro-Focusing Based on Sheath Flow

2012 ◽  
Vol 468-471 ◽  
pp. 1697-1701
Author(s):  
Rui Xia Yang ◽  
Chen Xin ◽  
Peng Gao ◽  
Fan Yang
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Chip ◽  
Glass Substrate ◽  
Silicon Etching ◽  
Microfluidic Chips ◽  
Hydrodynamic Focusing ◽  
Micro Electro Mechanical Systems ◽  
Process Step ◽  
Sheath Flow ◽  
Sharp Edges

Silicon etching is an essential process step for the fabrication of micro-electro-mechanical systems (MEMS). However, the rough edge of the silicon etching technology has some resistance to microspheres. This study presents fabrication process of the SU-8 photoresists mold which provides sharp edges and smooth sidewalls of the channel and details the RIE bonding of PDMS and glass substrate to realize the fabrication of microfluidic chips. This paper also investigates the hydrodynamic focusing and microsphere single-pass based on sheath flow with the aid of the plunger piston, which is important to the further combination of the microfluidic chip and the flow cytometry.

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