Quantum dots pushing up in vitro diagnostics limits

2017 ◽  
Author(s):  
Patricia M. A. Farias ◽  
Arnaldo C. D. S. Andrade ◽  
Raquel Milani ◽  
Yolice P. M. Ruiz ◽  
Terezinha Tabosa ◽  
...  
Keyword(s):  
Quantum Dots ◽  
In Vitro Diagnostics

scholarly journals Research of the peculiarities of colloidal quantum dots of AgInS2/ZnS and chitosan nanoparticles labeled with indocyanine green as the fluorescent labels for biomedical applications

2018 ◽  
Vol 17 (1) ◽  
pp. 74-82 ◽  
Author(s):  
M. S. Istomina ◽  
E. I. Pochkaeva ◽  
D. L. Sonin ◽  
N. A. Pechnikova ◽  
V. N. Postnov ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Biomedical Applications ◽  
Chitosan Nanoparticles ◽  
Colloidal Quantum Dots ◽  
Fluorescent Labels ◽  
In Vitro Diagnostics

The research is devoted to the investigating of the possibility of using colloidal quantum dots, covered by various layers and nanoparticles of chitosan labelled with indocyanin green for fluorescence imaging in vitro and in vivo. Pure quantum dots and dots in the shell synthesized from 3-aminopropyltriethoxysilane and in the shell of albumin were investigated. Research using fluorescence Visualizer IVIS Lumina LT Series III (Perkin Elmer, USA) was conducted. The possibility of using quantum dots for in vitro diagnostics, and chitosan nanoparticles - in vitro and in vivo was presented.

scholarly journals Nanoimprinted multifunctional nanoprobes for a homogeneous immunoassay in a top-down fabrication approach

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hubert Brueckl ◽  
Astrit Shoshi ◽  
Stefan Schrittwieser ◽  
Barbara Schmid ◽  
Pia Schneeweiss ◽  
...  
Keyword(s):  
Thin Film ◽  
Nanoimprint Lithography ◽  
Thin Film Deposition ◽  
Film Deposition ◽  
In Vitro Diagnostics ◽  
Top Down ◽  
Highly Sensitive ◽  
Optical And Magnetic Properties ◽  
Lift Off

AbstractMultifunctional nanoparticles are discussed as versatile probes for homogeneous immunoassays for in-vitro diagnostics. Top-down fabrication allows to combine and tailor magnetic and plasmonic anisotropic properties. The combination of nanoimprint lithography, thin film deposition, and lift-off processing provides a top-down fabrication platform, which is both flexible and reliable. Here, we discuss the material compositions and geometrical designs of monodisperse multicomponent nanoparticles and their consequences on optical and magnetic properties. The rotational hydrodynamics of nanoparticles is measured and considered under the influence of magnetic shape anisotropy in the framework of the Stoner-Wohlfarth theory. The plasmon-optical properties are explained by discrete-dipole finite-element simulations. Rotational dynamical measurements of imprinted nanoprobes for two test proteins demonstrate the applicability as highly sensitive biomolecular nanoprobes.

scholarly journals Effects of Doxorubicin Delivery by Nitrogen-Doped Graphene Quantum Dots on Cancer Cell Growth: Experimental Study and Mathematical Modeling

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 140
Author(s):  
Madison Frieler ◽  
Christine Pho ◽  
Bong Han Lee ◽  
Hana Dobrovolny ◽  
Giridhar R. Akkaraju ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Growth ◽  
Cancer Cell ◽  
Graphene Quantum Dots ◽  
Maximum Effect ◽  
Cancer Cell Growth ◽  
Nitrogen Doped ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

With 18 million new cases diagnosed each year worldwide, cancer strongly impacts both science and society. Current models of cancer cell growth and therapeutic efficacy in vitro are time-dependent and often do not consider the Emax value (the maximum reduction in the growth rate), leading to inconsistencies in the obtained IC50 (concentration of the drug at half maximum effect). In this work, we introduce a new dual experimental/modeling approach to model HeLa and MCF-7 cancer cell growth and assess the efficacy of doxorubicin chemotherapeutics, whether alone or delivered by novel nitrogen-doped graphene quantum dots (N-GQDs). These biocompatible/biodegradable nanoparticles were used for the first time in this work for the delivery and fluorescence tracking of doxorubicin, ultimately decreasing its IC50 by over 1.5 and allowing for the use of up to 10 times lower doses of the drug to achieve the same therapeutic effect. Based on the experimental in vitro studies with nanomaterial-delivered chemotherapy, we also developed a method of cancer cell growth modeling that (1) includes an Emax value, which is often not characterized, and (2), most importantly, is measurement time-independent. This will allow for the more consistent assessment of the efficiency of anti-cancer drugs and nanomaterial-delivered formulations, as well as efficacy improvements of nanomaterial delivery.

scholarly journals Synthesis and Characterization of Polyvinylpyrrolidone-Modified ZnO Quantum Dots and Their In Vitro Photodynamic Tumor Suppressive Action

2021 ◽  
Vol 22 (15) ◽  
pp. 8106
Author(s):  
Tianming Song ◽  
Yawei Qu ◽  
Zhe Ren ◽  
Shuang Yu ◽  
Mingjian Sun ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Photodynamic Therapy ◽  
Inhibitory Effect ◽  
Therapeutic Effects ◽  
In Vivo Experiments ◽  
Potential Applications ◽  
Zno Quantum Dots ◽  
Zno Qds

Despite the numerous available treatments for cancer, many patients succumb to side effects and reoccurrence. Zinc oxide (ZnO) quantum dots (QDs) are inexpensive inorganic nanomaterials with potential applications in photodynamic therapy. To verify the photoluminescence of ZnO QDs and determine their inhibitory effect on tumors, we synthesized and characterized ZnO QDs modified with polyvinylpyrrolidone. The photoluminescent properties and reactive oxygen species levels of these ZnO/PVP QDs were also measured. Finally, in vitro and in vivo experiments were performed to test their photodynamic therapeutic effects in SW480 cancer cells and female nude mice. Our results indicate that the ZnO QDs had good photoluminescence and exerted an obvious inhibitory effect on SW480 tumor cells. These findings illustrate the potential applications of ZnO QDs in the fields of photoluminescence and photodynamic therapy.

scholarly journals Nanotomography of Cell Surfaces with Evanescent Fields

2008 ◽  
Vol 2008 ◽  
pp. 1-7 ◽  
Author(s):  
Michael Wagner ◽  
Petra Weber ◽  
Wolfgang S. L. Strauss ◽  
Henri-Pierre Lassalle ◽  
Herbert Schneckenburger
Keyword(s):  
Total Internal Reflection ◽  
3D Imaging ◽  
Cell Swelling ◽  
Cholesterol Depletion ◽  
In Vitro Diagnostics ◽  
Axial Resolution ◽  
Cell Surfaces ◽  
Cell Substrate ◽  
Cell Topology

The technique of variable-angle total internal reflection fluorescence microscopy (TIRFM) and its application to nanotomography of cell surfaces are described. Present applications include (1) 3D imaging of chromosomes in their metaphase to demonstrate axial resolution in the nanometre range, (2) measurements of cell-substrate topology, which upon cholesterol depletion shows some loosening of cell-substrate contacts, and (3) measurements of cell topology upon photodynamic therapy (PDT), which demonstrate cell swelling and maintenance of focal contacts. The potential of the method for in vitro diagnostics, but also some requirements and limitations are discussed.

Design and Construction of Three-Dimensional Physiologically-Based Vascular Branching Networks for Respiratory Assist Devices

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Jose Santos ◽  
Alla A Gimbel ◽  
Athanasios Peppas ◽  
James G Truslow ◽  
Daniel Lang ◽  
...  
Keyword(s):  
Drug Development ◽  
Three Dimensional ◽  
Lab On A Chip ◽  
In Vitro Diagnostics ◽  
Assist Devices ◽  
Branching Networks ◽  
Physiologically Based ◽  
Vascular Branching ◽  
Design And Construction

Microfluidic lab-on-a-chip devices are changing the way that in vitro diagnostics and drug development are conducted, based on the increased precision, miniaturization and efficiency of these systems relative to prior...

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