Towards laser-based open-path detection of hydrogen sulfide

2016 ◽  
Author(s):  
Michal Nikodem ◽  
Dorota Stachowiak ◽  
Piotr Jaworski
Keyword(s):  
Hydrogen Sulfide ◽  
Open Path ◽  
Path Detection

Research on Open-Path Detection for Atmospheric Trace Gas CO Based on TDLAS

2015 ◽  
Vol 42 (2) ◽  
pp. 0215003 ◽  
Author(s):  
姚路 Yao Lu ◽  
刘文清 Liu Wenqing ◽  
刘建国 Liu Jianguo ◽  
阚瑞峰 Kan Ruifeng ◽  
许振宇 Xu Zhenyu ◽  
...  
Keyword(s):  
Trace Gas ◽  
Open Path ◽  
Atmospheric Trace Gas ◽  
Path Detection

An optical sensor for hydrogen sulfide detection in open path using WMS-2f/1f technique

2016 ◽  
Vol 12 (6) ◽  
pp. 465-468 ◽  
Author(s):  
Li-mei Song ◽  
Li-wen Liu ◽  
Yan-gang Yang ◽  
Qing-hua Guo ◽  
Jiang-tao Xi
Keyword(s):  
Hydrogen Sulfide ◽  
Optical Sensor ◽  
Open Path

Open-path Detection Of Atmospheric CH4 And N2O Based On Quantum Cascade Laser

2014 ◽  
Author(s):  
Min Wei ◽  
JianGuo Liu ◽  
RuiFeng Kan ◽  
Wei Wang ◽  
Sun Liqin ◽  
...  
Keyword(s):  
Quantum Cascade Laser ◽  
Quantum Cascade ◽  
Open Path ◽  
Ch4 And N2o ◽  
Path Detection

Simultaneous Open-path Detection of Atmospheric N2O and CO

2012 ◽  
Author(s):  
Lei Tao ◽  
Kang Sun ◽  
M. Amir Khan ◽  
David J. Miller ◽  
Mark A. Zondlo
Keyword(s):  
Open Path ◽  
Path Detection

scholarly journals Standoff 250 m Open-path Detection of Chemical Plumes Using a Broadband Swept-ECQCL

2019 ◽  
Author(s):  
Mark C. Phillips ◽  
Bruce E. Bernacki ◽  
Sivanandan S. Harilal ◽  
Jeremy Yeak ◽  
R. Jason Jones
Keyword(s):  
Open Path ◽  
Path Detection

Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Cell Model ◽  
Site Directed Mutagenesis ◽  
Critical Event ◽  
Hypertensive Rats ◽  
Pulmonary Artery Endothelial Cell

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.

Sign in / Sign up

Export Citation Format

Share Document