Molecular imaging of melanin distribution in vivo and quantitative differential diagnosis of human pigmented lesions using label-free harmonic generation biopsy (Conference Presentation)

2017 ◽  
Author(s):  
Chi-Kuang Sun ◽  
Ming-Liang Wei ◽  
Yu-Hsiang Su ◽  
Wei-Hung Weng ◽  
Yi-Hua Liao
Keyword(s):  
Differential Diagnosis ◽  
Molecular Imaging ◽  
Harmonic Generation ◽  
Label Free ◽  
Pigmented Lesions

scholarly journals Label-free in vivo molecular imaging of underglycosylated mucin-1 expression in tumour cells

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaolei Song ◽  
Raag D. Airan ◽  
Dian R. Arifin ◽  
Amnon Bar-Shir ◽  
Deepak K. Kadayakkara ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Tumour Cells ◽  
Label Free ◽  
Mucin 1

scholarly journals Second harmonic generation microscopy is a novel technique for differential diagnosis of breast fibroepithelial lesions

2015 ◽  
Vol 68 (12) ◽  
pp. 1033-1035 ◽  
Author(s):  
Wai Jin Tan ◽  
Jie Yan ◽  
Shuoyu Xu ◽  
Aye Aye Thike ◽  
Boon Huat Bay ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Clinical Decision Making ◽  
Direct Detection ◽  
Second Harmonic ◽  
Collagen I ◽  
Tissue Array ◽  
Label Free ◽  
Phyllodes Tumours

Breast fibroepithelial lesions, including fibroadenomas and phyllodes tumours, are commonly encountered in clinical practice. As histological differences between these two related entities may be subtle, resulting in a challenging differential diagnosis, pathological techniques to assist the differential diagnosis of these two entities are of high interest. An accurate diagnosis at biopsy is important given corresponding implications for clinical decision-making including surgical extent and monitoring. Second harmonic generation (SHG) microscopy is a recently developed optical imaging technique capable of robust, powerful and unbiased label-free direct detection of collagen fibril structure in tissue without the use of antibodies. We constructed tissue microarrays emulating limited materials on biopsy to investigate quantitative collagen signal in fibroepithelial lesions using SHG microscopy. Archived formalin-fixed paraffin-embedded materials of 47 fibroepithelial lesions (14 fibroadenomas and 33 phyllodes tumours) were evaluated. Higher collagen signal on SHG microscopy was observed in fibroadenomas than phyllodes tumours on SHG imaging (p<0.001, area under the curve 0.859). At an automated threshold (2.5 million positive pixels), the sensitivity and specificity of the SHG microscopy for fibroadenoma classification was 71.4% and 84.4%, respectively. To corroborate these findings, we performed immunohistochemistry on tissue array sections using collagen I and III primary antibodies. Both collagen I and III immunohistochemical expressions were also significantly higher in fibroadenomas than in phyllodes tumours (p<0.001). In conclusion, label-free collagen quantitation on SHG microscopy is a novel imaging approach that can aid the differential diagnosis of fibroepithelial lesions.

scholarly journals 3-photon fluorescence and third-harmonic generation imaging of myelin sheaths in mouse digital skin in vivo: A comparative study

2021 ◽  
Author(s):  
Ke Wang ◽  
Yi Pan ◽  
Xinlin Chen ◽  
Shen Tong ◽  
Huiping Liang ◽  
...  
Keyword(s):  
Harmonic Generation ◽  
Fluorescent Dyes ◽  
Propagation Speed ◽  
Third Harmonic Generation ◽  
Label Free ◽  
Third Harmonic ◽  
Myelin Sheaths ◽  
Deep Imaging ◽  
Photon Fluorescence

Myelin sheaths wrapping axons are key structures that help maintain the propagation speed of action potentials in both central and peripheral nervous systems (CNS and PNS). However, noninvasive, deep imaging technologies visualizing myelin sheaths in the digital skin in vivo are lacking in animal models. 3-photon fluorescence (3PF) imaging excited at the 1700-nm window enables deep imaging of myelin sheaths, but necessitates labeling by exogenous fluorescent dyes. Since myelin sheaths are lipid-rich structures which generate strong third-harmonic signals, in this paper, we perform a detailed comparative experimental study of both third-harmonic generation (THG) and 3PF imaging in the mouse digital skin in vivo. Our results show that THG imaging also enables visualization of myelin sheaths deep in the mouse digital skin, which shows colocalization with 3PF signals from labeled myelin sheaths. Besides its superior label-free advantage, THG does not suffer from photobleaching due to its 3PF property.

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