Recent advances in high-throughput QCL-based infrared microspectral imaging (Conference Presentation)

2017 ◽  
Author(s):  
Jeremy A. Rowlette ◽  
Edeline Fotheringham ◽  
David Nichols ◽  
Miles J. Weida ◽  
Justin Kane ◽  
...  
Keyword(s):  
High Throughput ◽  
Recent Advances

scholarly journals Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation

2020 ◽  
Author(s):  
Suraj Makhija ◽  
David Brown ◽  
Struan Bourke ◽  
Yina Wang ◽  
Shuqin Zhou ◽  
...  
Keyword(s):  
Protein Expression ◽  
High Throughput ◽  
Protein Interaction ◽  
Genome Engineering ◽  
Small Peptide ◽  
Protein Protein Interaction ◽  
Recent Advances ◽  
Cell Enrichment ◽  
Peptide Tags ◽  
Endogenous Proteins

AbstractRecent advances in genome engineering have expanded our capabilities to study proteins in their natural states. In particular, the ease and scalability of knocking-in small peptide tags has enabled high throughput tagging and analysis of endogenous proteins. To improve enrichment capacities and expand the functionality of knock-ins using short tags, we developed the tag-assisted split enzyme complementation (TASEC) approach, which uses two orthogonal small peptide tags and their cognate binders to conditionally drive complementation of a split enzyme upon labeled protein expression. Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited cells through multiplexing.

scholarly journals RNA interactomics: recent advances and remaining challenges

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1824 ◽  
Author(s):  
Brigitte Schönberger ◽  
Christoph Schaal ◽  
Richard Schäfer ◽  
Björn Voß
Keyword(s):  
High Throughput ◽  
Rna Structure ◽  
Living Cells ◽  
Structure And Function ◽  
Cellular Processes ◽  
Recent Advances ◽  
Regulatory Systems ◽  
Rna Duplexes ◽  
And Function

Tight regulation of cellular processes is key to the development of complex organisms but also vital for simpler ones. During evolution, different regulatory systems have emerged, among them RNA-based regulation that is carried out mainly by intramolecular and intermolecular RNA–RNA interactions. However, methods for the transcriptome-wide detection of these interactions were long unavailable. Recently, three publications described high-throughput methods to directly detect RNA duplexes in living cells. This promises to enable in-depth studies of RNA-based regulation and will narrow the gaps in our understanding of RNA structure and function. In this review, we highlight the benefits of these methods and their commonalities and differences and, in particular, point to methodological shortcomings that hamper their wider application. We conclude by presenting ideas for how to overcome these problems and commenting on the prospects we see in this area of research.

scholarly journals Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

2019 ◽  
Vol 29 (1) ◽  
pp. 133-149
Author(s):  
Khosro Balilashaki ◽  
Hedayat Zakizadeh ◽  
Jamal Ali Olfati ◽  
Maryam Vahedi ◽  
Anuj Kumar ◽  
...  
Keyword(s):  
High Throughput ◽  
Plant Tissue ◽  
Industrial Production ◽  
High Throughput Sequencing ◽  
Genomic Resources ◽  
Recent Advances ◽  
Recent Developments

With recent advances in high-throughput sequencing (HTS) technologies to improve plants, there is a need to release orchid specific genomic resources and platforms that are crucial for managing omics elements in systematic manner. Authors provide details about the recent developments in biotechnological techniques, genomics, transcriptomics, proteomics, metabolomics and their applications for the industrial production, propagation, conservation and manipulation of Phalaenopsis orchid. Plant Tissue Cult. & Biotech. 29(1): 133-149, 2019 (June)

scholarly journals Recent advances and potential applications of human pluripotent stem cell-derived pancreatic β cells

2020 ◽  
Vol 52 (7) ◽  
pp. 708-715 ◽  
Author(s):  
Ziyu Zhou ◽  
Xiaojie Ma ◽  
Saiyong Zhu
Keyword(s):  
High Throughput ◽  
Disease Modeling ◽  
Patient Specific ◽  
Target Cells ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
High Blood Glucose ◽  
Chemical Screening ◽  
Recent Advances ◽  
Potential Applications

Abstract Diabetes mellitus is characterized by chronic high blood glucose levels resulted from deficiency and/or dysfunction of insulin-producing pancreatic β cells. Generation of large amounts of functional pancreatic β cells is critical for the study of pancreatic biology and treatment of diabetes. Recent advances in directed differentiation of pancreatic β-like cells from human pluripotent stem cells (hPSCs) can provide patient-specific and disease-relevant target cells. With the improved differentiation protocols, it is now possible to generate large amounts of functional human pancreatic β-like cells that can response to high level of glucose both in vitro and in vivo. Combined with precise genomic editing, biomedical engineering, high throughput profiling, bioinformatics, and high throughput genetic and chemical screening, these hPSC-derived pancreatic β-like cells will hold great potentials in disease modeling, drug discovery, and cell-based therapies. In this review, we summarize the recent progress in human pancreatic β-like cells derived from hPSCs and discuss their potential applications.

scholarly journals Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 436 ◽  
Author(s):  
Varvara Maliogka ◽  
Angelantonio Minafra ◽  
Pasquale Saldarelli ◽  
Ana Ruiz-García ◽  
Miroslav Glasa ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Infected Plant ◽  
Fruit Trees ◽  
Plant Viruses ◽  
Fruit Tree ◽  
Advantages And Disadvantages ◽  
Sequencing Technologies ◽  
Recent Advances

Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., Fabavirus, Luteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed.

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