scholarly journals Quantitative assessment of graded burn wounds using a commercial and research grade laser speckle imaging (LSI) system

2017 ◽  
Author(s):  
A. Ponticorvo ◽  
R. Rowland ◽  
B. Yang ◽  
B. Lertsakdadet ◽  
C. Crouzet ◽  
...  
Keyword(s):  
Quantitative Assessment ◽  
Laser Speckle ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
Burn Wounds ◽  
Research Grade

516 Determination of Adequate Debridement of Burn Wounds via Laser Speckle Imaging

2019 ◽  
Vol 40 (Supplement_1) ◽  
pp. S234-S234
Author(s):  
R Stone ◽  
D Larson ◽  
J Wall ◽  
H Dillon ◽  
C Kowalczewski ◽  
...  
Keyword(s):  
Laser Speckle ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
Burn Wounds

scholarly journals Enzymatic Debridement of Porcine Burn Wounds via a Novel Protease, SN514

2020 ◽  
Vol 41 (5) ◽  
pp. 1015-1028
Author(s):  
Randolph Stone ◽  
Angela R Jockheck-Clark ◽  
Shanmugasundaram Natesan ◽  
Julie A Rizzo ◽  
Nathan A Wienandt ◽  
...  
Keyword(s):  
Thermal Injury ◽  
Laser Speckle ◽  
Necrotic Tissue ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
Burn Wounds ◽  
Clinical Observations ◽  
Extended Care ◽  
Gel Treatment

Abstract Necrotic tissue generated by a thermal injury is typically removed via surgical debridement. However, this procedure is commonly associated with blood loss and the removal of viable healthy tissue. For some patients and contexts such as extended care on the battlefield, it would be preferable to remove devitalized tissue with a nonsurgical debridement agent. In this paper, a proprietary debridement gel (SN514) was evaluated for the ability to debride both deep-partial thickness (DPT) and full-thickness burn wounds using an established porcine thermal injury model. Burn wounds were treated daily for 4 days and visualized with both digital imaging and laser speckle imaging. Strip biopsies were taken at the end of the procedure. Histological analyses confirmed a greater debridement of the porcine burn wounds by SN514 than the vehicle-treated controls. Laser speckle imaging detected significant increases in the perfusion status after 4 days of SN514 treatment on DPT wounds. Importantly, histological analyses and clinical observations suggest that SN514 gel treatment did not damage uninjured tissue as no edema, erythema, or inflammation was observed on intact skin surrounding the treated wounds. A blinded evaluation of the digital images by a burn surgeon indicated that SN514 debrided more necrotic tissue than the control groups after 1, 2, and 3 days of treatment. Additionally, SN514 gel was evaluated using an in vitro burn model that used human discarded skin. Treatment of human burned tissue with SN514 gel resulted in greater than 80% weight reduction compared with untreated samples. Together, these data demonstrate that SN514 gel is capable of debriding necrotic tissue and suggest that SN514 gel could be a useful option for austere conditions, such as military multi-domain operations and prolonged field care scenarios.

scholarly journals Simple correction factor for laser speckle imaging of flow dynamics

2014 ◽  
Vol 39 (3) ◽  
pp. 678 ◽  
Author(s):  
J. C. Ramirez-San-Juan ◽  
R. Ramos-Garcia ◽  
G. Martinez-Niconoff ◽  
B. Choi
Keyword(s):  
Correction Factor ◽  
Flow Dynamics ◽  
Laser Speckle ◽  
Laser Speckle Imaging ◽  
Speckle Imaging

Laser speckle imaging reveals bacterial activity within colony

2021 ◽  
Author(s):  
Ilya Balmages ◽  
Janis Liepins ◽  
Dmitrijs Bliznuks ◽  
Stivens Zolins ◽  
Ilze Lihacova ◽  
...  
Keyword(s):  
Bacterial Activity ◽  
Laser Speckle ◽  
Laser Speckle Imaging ◽  
Speckle Imaging

scholarly journals Application of optical flow algorithms to laser speckle imaging

2019 ◽  
Vol 122 ◽  
pp. 52-59 ◽  
Author(s):  
AmirHessam Aminfar ◽  
Nami Davoodzadeh ◽  
Guillermo Aguilar ◽  
Marko Princevac
Keyword(s):  
Optical Flow ◽  
Laser Speckle ◽  
Laser Speckle Imaging ◽  
Speckle Imaging

scholarly journals Fourier transforms for fast and quantitative Laser Speckle Imaging

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
J. Buijs ◽  
J. van der Gucht ◽  
J. Sprakel
Keyword(s):  
Quantitative Data ◽  
Fourier Transforms ◽  
Imaging Technique ◽  
Laser Speckle ◽  
Frequency Range ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
New Approach ◽  
Speckle Patterns ◽  
New Processing

Abstract Laser speckle imaging is a powerful imaging technique that visualizes microscopic motion within turbid materials. At current two methods are widely used to analyze speckle data: one is fast but qualitative, the other quantitative but computationally expensive. We have developed a new processing algorithm based on the fast Fourier transform, which converts raw speckle patterns into maps of microscopic motion and is both fast and quantitative, providing a dynamnic spectrum of the material over a frequency range spanning several decades. In this article we show how to apply this algorithm and how to measure a diffusion coefficient with it. We show that this method is quantitative and several orders of magnitude faster than the existing quantitative method. Finally we harness the potential of this new approach by constructing a portable laser speckle imaging setup that performs quantitative data processing in real-time on a tablet.

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