An infrared optical pacing system for high-throughput screening of cardiac electrophysiology in human cardiomyocytes (Conference Presentation)

2017 ◽  
Author(s):  
Matthew T. McPheeters ◽  
Yves T. Wang ◽  
Kenneth R. Laurita ◽  
Michael W. Jenkins
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Cardiac Electrophysiology ◽  
Human Cardiomyocytes

scholarly journals OptoDyCE: Automated System for High-Throughput All-Optical Dynamic Cardiac Electrophysiology

2015 ◽  
Author(s):  
Aleksandra Klimas ◽  
Jinzhu Yu ◽  
Christina M. Ambrosi ◽  
John C. Williams ◽  
Harold Bien ◽  
...  
Keyword(s):  
High Throughput ◽  
Cardiac Electrophysiology ◽  
Drug Testing ◽  
Dynamic Testing ◽  
Cellular Level ◽  
Automated System ◽  
Human Cardiomyocytes ◽  
Tissue Constructs ◽  
Time Space ◽  
All Optical

The improvement of preclinical cardiotoxicity testing, the discovery of new ion channel-targeted drugs, and the phenotyping and use of stem-cell-derived cardiomyocytes and other biologics all necessitate high-throughput (HT), cellular level electrophysiological interrogation tools. Optical techniques for actuation and sensing provide instant parallelism, enabling contactless dynamic HT testing of cells and small-tissue constructs, not affordable by other means. Here, we consider, computationally and experimentally, the limits of all-optical electrophysiology when applied to drug testing, then implement and validate OptoDyCE, a fully automated system for all-optical cardiac electrophysiology. We validate optical actuation by virally introducing optogenetic drivers in (rat and human) cardiomyocytes or through the modular use of dedicated light-sensitive somatic "spark" cells. We show that this automated all-optical approach provides high-throughput means of cellular interrogation, i.e. allows for dynamic testing of >600 multicellular samples or compounds per hour, and yields high-content information about the action of a drug over time, space and doses.

Breaking new ground in early cardiac safety: Human cardiomyocytes derived from iPSCs in high-throughput screening

2015 ◽  
Vol 75 ◽  
pp. 197
Author(s):  
Ralf Kettenhofen ◽  
Anika Duenbostell ◽  
Thomas Niedereichholz ◽  
Jean Marc D'Angelo ◽  
Hirofumi Horai ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Cardiac Safety ◽  
Human Cardiomyocytes

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome

High-throughput screening revealed a clinically relevant drug to induce sperm motility

2014 ◽  
Author(s):  
Clair Cochrane ◽  
Halil Ruso ◽  
Anthony Hope ◽  
Rosemary G Clarke ◽  
Christopher Barratt ◽  
...  
Keyword(s):  
Sperm Motility ◽  
High Throughput ◽  
High Throughput Screening
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