Low-cost production and sealing procedure of mechanical parts of a versatile 3D-printed perfusion chamber for digital holographic microscopy of primary neurons in culture

2017 ◽  
Author(s):  
Erik Bélanger ◽  
Sébastien A. Lévesque ◽  
Gabriel Anctil ◽  
Anne-Sophie Poulin-Girard ◽  
Pierre Marquet
Keyword(s):  
Low Cost ◽  
Digital Holographic Microscopy ◽  
Primary Neurons ◽  
Mechanical Parts ◽  
Perfusion Chamber ◽  
3D Printed ◽  
Holographic Microscopy

scholarly journals Single-Element Reflective Digital Holographic Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
José Ángel Picazo-Bueno ◽  
Javier García ◽  
Vicente Micó
Keyword(s):  
Low Cost ◽  
Digital Holographic Microscopy ◽  
Single Element ◽  
Phase Imaging ◽  
Single Shot ◽  
One Dimensional ◽  
Common Path ◽  
Microscopy Technique ◽  
Digital Holograms ◽  
Holographic Microscopy

Digital holographic microscopy (DHM) is a well-known microscopy technique using an interferometric architecture for quantitative phase imaging (QPI) and it has been already implemented utilizing a large number of interferometers. Among them, single-element interferometers are of particular interest due to its simplicity, stability, and low cost. Here, we present an extremely simple common-path interferometric layout based on the use of a single one-dimensional diffraction grating for both illuminating the sample in reflection and generating the digital holograms. The technique, named single-element reflective digital holographic microscopy (SER-DHM), enables QPI and topography analysis of reflective/opaque objects using a single-shot operation principle. SER-DHM is experimentally validated involving different reflective samples.

scholarly journals Low cost production of 3D-printed devices and electrostimulation chambers for the culture of primary neurons

2015 ◽  
Vol 251 ◽  
pp. 17-23 ◽  
Author(s):  
Joanna D. Wardyn ◽  
Chris Sanderson ◽  
Laura E. Swan ◽  
Massimiliano Stagi
Keyword(s):  
Low Cost ◽  
Primary Neurons ◽  
3D Printed
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