Advanced quantitative imaging of musculoskeletal disorders (Conference Presentation)

Author(s):  
Rajeev Chaudhary ◽  
Matthew Halanski ◽  
Paul J. Campagnola
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Judith E. Gold ◽  
David M. Hallman ◽  
Fredrik Hellström ◽  
Martin Björklund ◽  
Albert G. Crenshaw ◽  
...  

Author(s):  
Leslie M. Loew

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.


2000 ◽  
Vol 81 (3B) ◽  
pp. s73-s77
Author(s):  
Darryl L. Kaelin ◽  
Terry H. Oh ◽  
Peter A.C. Lim ◽  
Victoria A. Brander ◽  
Joseph J. Biundo

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