Ex-vivo UV autofluorescence imaging and fluorescence spectroscopy of atherosclerotic pathology in human aorta

2017 ◽  
Author(s):  
William Lewis ◽  
Maura Williams ◽  
Walfre Franco
Keyword(s):  
Fluorescence Spectroscopy ◽  
Ex Vivo ◽  
Human Aorta ◽  
Autofluorescence Imaging

scholarly journals Biomechanical simulations and 3D printing for endovascular device testing

2020 ◽  
Author(s):  
Michele Conti ◽  
Stefania Marconi ◽  
Ferdinando Auricchio
Keyword(s):  
3D Printing ◽  
Mechanical Response ◽  
Ex Vivo ◽  
Invasive Procedure ◽  
Patient Specific ◽  
Human Aorta ◽  
In Vitro Tests ◽  
Aortic Diseases ◽  
Endovascular Device

Endovascular aortic repair is a minimally invasive procedure to treat aortic diseases such as aneurysms and dissections. Thanks to technological advancements, such procedure has steadily shifted from the abdominal aorta towards the ascending part, i.e., near the heart, calling for an extensive and comprehensive benchmarking of (novel) endografts. Given such considerations, we have exploited porcine aorta with a pulse duplicator to analyse the mechanical interaction between the endovascular device and the native tissue. Our results have implications for using the porcine aorta as a model for human aorta in research. Particularly, the combination of in vitro tests performed using ex-vivo tissue, integrated validated patient-specific numerical simulations, mock arteries manufactured by 3D printing, can offer important insight on biomechanical impact of endograft design on post-operative aortic mechanical response.

ALTERATIONS IN AUTOFLUORESCENCE SIGNAL FROM RAT SKIN EX VIVO UNDER OPTICAL IMMERSION CLEARING

2010 ◽  
Vol 03 (03) ◽  
pp. 147-152 ◽  
Author(s):  
E. V. MIGACHEVA ◽  
A. B. PRAVDIN ◽  
V. V. TUCHIN
Keyword(s):  
Fluorescence Spectroscopy ◽  
Ex Vivo ◽  
Rat Skin ◽  
Optical Clearing ◽  
Spectral Curves ◽  
The Common ◽  
First Time ◽  
Tissue Optical Clearing ◽  
Optical Immersion

For the first time, the changes in autofluorescence spectra of ex vivo rat skin have been experimentally investigated using the combination of fluorescence spectroscopy and optical immersion clearing. The glucose, glycerol and propylene glycol solutions were used as clearing agents. The optical clearing was performed from the dermal side of skin imitating the in vivo injection of clearing agent under the dermal layers. In this contribution, the common properties of autofluorescence variation during optical immersion clearing were determined. The tendency of autofluorescence signal to decrease with reduction of scattering in tissue was noticed and discussed in detail. However, the differences in the shape of spectral curves under application of different clearing agents showed that optical clearing affects the autofluorescence properties of tissue differently depending on the type of clearing liquid. The results obtained are useful for the understanding of tissue optical clearing mechanisms and for improving techniques such as fluorescence spectroscopy.

scholarly journals Double Assurance of Epidural Space Detection Using Fiberoptics-Based Needle Design and Autofluorescence Technologies for Epidural Blockade in Painless Labor

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3592
Author(s):  
Cihun-Siyong Gong ◽  
Huang-Chang Lee ◽  
Yin Chang ◽  
Chien-Kun Ting ◽  
Po-Hsun Tu
Keyword(s):  
Fluorescence Spectroscopy ◽  
Epidural Space ◽  
Ex Vivo ◽  
In Vivo Study ◽  
Epidural Needle ◽  
Needle Puncture ◽  
Double Peak ◽  
Interspinous Ligament ◽  
Auto Fluorescence

Purpose: Technology of reflectance spectroscopy incorporated with auto-fluorescence spectroscopy were employed to increase the safety of epidural placement in regional anesthesia which is generally used for surgery, epidural anesthesia, post-operative pain control and painless childbirth. Method: Ex vivo study of auto-fluorescence spectroscopy was performed for the para-vertebral tissues contained fat, interspinous ligament, supraspinous ligament and ligamentumflavum by multimode microplate reader at wavelength 405 nm for the purpose of tissue differentiation. A specially designed optic-fiber-embedded needle was employed to incorporate with both reflectance and autofluorescence spectroscopies in order to probe the epidural space as double assurance demands. In vivo study was carried out in a Chinese native swine weighted about 30 kg under intubated general anesthesia with ventilation support. The reflective (405 nm) and autofluorescence signals (λ and λ*) were recorded at 5 different sites by an oscilloscope during the needle puncture procedure from skin to epidural space in the back of the swine. Results: Study of either autofluorescence spectroscopy for tissue samples or ex vivo needle puncture in porcine trunk tissues indicates that ligmentumflavum has at least 10-fold higher fluorescence intensity than the other tissues. In the in vivo study, ligamentumflavum shows a double-peak character for both reflectance and autofluorescence signals. The epidural space is located right after the drop from the double-peak. Both peaks of reflectance and fluorescence are coincident which ensures that the epidural space is correctly detected. Conclusions: The fiber-optical technologies of double-assurance demands for tissue discrimination during epidural needle puncture can not only provide an objective visual information in a real-time fashion but also it can help the operator to achieve much higher success rate in this anesthesia procedure.

scholarly journals Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry

2018 ◽  
Vol 7 (3) ◽  
pp. 4-20 ◽  
Author(s):  
J. O. Zolotareva ◽  
D. S. Farrakhova ◽  
E. N. Kupriyanova ◽  
V. B. Loschenov
Keyword(s):  
Early Diagnosis ◽  
Fluorescence Spectroscopy ◽  
Model Compound ◽  
Colloidal Solution ◽  
Tooth Enamel ◽  
Ex Vivo ◽  
Molecular Form ◽  
Statistical Processing ◽  
Enamel Surface ◽  
Aluminum Phthalocyanine

Early diagnosis of caries and tooth enamel microcracks is of great importance for preventing the destruction of healthy tooth enamel. Inorder to detect microcracks in the enamel and pathogenic microflora foci that can cause caries, nanoform of aluminum phthalocyanine (AlPc) can be used as a marker. In a colloidal solution, the nanoparticles do not fluoresce, unlike their molecular form. To convert the particle into its molecular form, it is necessary to have a solvent or specific environment (bacteria, macrophages, etc.). That is why the hydrophobic nanoparticles of aluminum phthalocyanine (nAlPc) can act as markers for detecting hidden pathogenic microflora during fluorescent diagnostics. Further reduction of the diagnosis time and increase the efficiency can be achieved by using biologically compatible surfactants as additional activators of nAlPc.In order to carry out local fluorescence spectroscopy of enamel microcracks and pathogenic microflora foci on the enamel surface, a model compound containing surfactants, auxiliary components and nAlPc colloid at a concentration of 10 mg/l was prepared.Studies on the interaction of the model compound with nAlPc and Protelan MST-35 with tooth enamel ex vivo have shown this surfactant to be a promising auxiliary activator of the nanoparticles, allowing conducting local fluorescence spectroscopy of the tooth enamel surface 3 min after application. In addition, statistical processing of the results showed the effectiveness of using the model compound for local fluorescence spectroscopy of the enamel surface in order to detect the enamel microcracks and the pathogenic microflora accumulation foci that can lead to the development of a cariogenic process.

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