Novel molecular-based fluorescent nanoparticles for three-photon excited microscopy at 1700 nm (Conference Presentation)

2017 ◽  
Author(s):  
Charles-Henri Hage ◽  
Patrick Cadroas ◽  
Jonathan Daniel ◽  
Paolo Pagano ◽  
Christiano Mastrodonato ◽  
...  
Keyword(s):  
Fluorescent Nanoparticles

Fluorescent Nanotechnology: An Evolution in Optical Sensors

2020 ◽  
Vol 17 ◽  
Author(s):  
Dilawar Hassan ◽  
Hadi Bakhsh ◽  
Asif M. Khurram ◽  
Shakeel A. Bhutto ◽  
Nida S. Jalbani ◽  
...  
Keyword(s):  
Optical Properties ◽  
Optical Sensors ◽  
Light Emission ◽  
Environmental Contaminants ◽  
Fluorescent Nanoparticles ◽  
Sensing Applications ◽  
Properties Of Materials ◽  
Optical Properties Of Materials ◽  
Fluorescent Nanomaterials

Background: The optical properties of nanomaterials have evolved enormously with the introduction of nanotechnology. The property of materials to absorb and/or emit specific wavelength has turned them into one of the most favourite candidates to be effectively utilized in different sensing applications e.g organic light emission diodes (OLEDs) sensors, gas sensors, biosensors and fluorescent sensors. These materials have been reported as a sensor in the field of tissue and cell imaging, cancer detection and detection of environmental contaminants etc. Fluorescent nanomaterials are heling in rapid and timely detection of various contaminants that greatly impact the quality of life and food, that is exposed to these contaminants. Later, all the contaminants have been investigated to be most perilous entities that momentously affect the life span of the animals and humans who use those foods which have been contaminated. Objective: In this review, we will discuss about various methods and approaches to synthesize the fluorescent nanoparticles and quantum dots (QDs) and their applications in various fields. The application will include the detection of various environmental contaminants and bio-medical applications. We will discuss the possible mode of action of the nanoparticles when used as sensor for the environmental contaminants as well as the surface modification of some fluorescent nanomaterials with anti-body and enzyme for specific detection in animal kingdom. We will also describe some RAMAN based sensors as well as some optical sensing-based nanosensors. Conclusion: Nanotechnology has enabled to play with the size, shape and morphology of materials in the nanoscale. The physical, chemical and optical properties of materials change dramatically when they are reduced to nanoscale. The optical properties can become choosy in terms of emission or absorption of wavelength in the size range and can result in production of very sensitive optical sensor. The results show that the use of fluorescent nanomaterials for the sensing purposes are helping a great deal in the sensing field.

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

scholarly journals Fabrication of polyethylene terephthalate (PET) nanoparticles with fluorescent tracers for studies in mammalian cells

2021 ◽  
Author(s):  
Leah M. Johnson ◽  
Jeffrey B. Mecham ◽  
Sai Archana Krovi ◽  
Maria M. Moreno Caffaro ◽  
Shyam Aravamudhan ◽  
...  
Keyword(s):  
Polyethylene Terephthalate ◽  
Mammalian Cells ◽  
Hydrodynamic Diameter ◽  
Fluorescent Nanoparticles ◽  
Fluorescent Tracers ◽  
Bottom Up

Fluorescent nanoparticles (NPs) comprising polyethylene terephthalate (PET) with a hydrodynamic diameter of 158 ± 2 nm were synthesized in a bottom-up approach.

scholarly journals Picosecond time-resolved photon antibunching measures nanoscale exciton motion and the true number of chromophores

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gordon J. Hedley ◽  
Tim Schröder ◽  
Florian Steiner ◽  
Theresa Eder ◽  
Felix J. Hofmann ◽  
...  
Keyword(s):  
Rational Design ◽  
Three Dimensional ◽  
Dna Origami ◽  
Fluorescent Nanoparticles ◽  
Time Resolved ◽  
Exciton Diffusion ◽  
Polymer Aggregates ◽  
True Number ◽  
Particle Level ◽  
Photon Antibunching

AbstractThe particle-like nature of light becomes evident in the photon statistics of fluorescence from single quantum systems as photon antibunching. In multichromophoric systems, exciton diffusion and subsequent annihilation occurs. These processes also yield photon antibunching but cannot be interpreted reliably. Here we develop picosecond time-resolved antibunching to identify and decode such processes. We use this method to measure the true number of chromophores on well-defined multichromophoric DNA-origami structures, and precisely determine the distance-dependent rates of annihilation between excitons. Further, this allows us to measure exciton diffusion in mesoscopic H- and J-type conjugated-polymer aggregates. We distinguish between one-dimensional intra-chain and three-dimensional inter-chain exciton diffusion at different times after excitation and determine the disorder-dependent diffusion lengths. Our method provides a powerful lens through which excitons can be studied at the single-particle level, enabling the rational design of improved excitonic probes such as ultra-bright fluorescent nanoparticles and materials for optoelectronic devices.

scholarly journals Novel methods for the rapid detection of trace tetracyclines based on the fluorescence behaviours of Maillard reaction fluorescent nanoparticles

RSC Advances ◽  
2020 ◽  
Vol 10 (71) ◽  
pp. 43256-43261
Author(s):  
Xue-jing Si ◽  
Hong-ling Wang ◽  
Tun-hua Wu ◽  
Ping Wang
Keyword(s):  
Food Safety ◽  
Maillard Reaction ◽  
Rapid Detection ◽  
Fluorescent Nanoparticles ◽  
Fluorescent Products ◽  
Novel Methods

The Maillard reaction and its fluorescent products have attracted widespread attention in the field of food safety and biology.

Fluorescent nanoparticles as tools in ecology and physiology

2021 ◽  
Author(s):  
Sanni M. A. Färkkilä ◽  
E. Toby Kiers ◽  
Raivo Jaaniso ◽  
Uno Mäeorg ◽  
Roger M. Leblanc ◽  
...  
Keyword(s):  
Fluorescent Nanoparticles

Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles

Nanoscale ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 3275-3284
Author(s):  
Huan Zhao ◽  
Qifeng Lin ◽  
Li Huang ◽  
Yunfeng Zhai ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Human Body ◽  
Accurate Diagnosis ◽  
Fluorescent Nanoparticles ◽  
Internal Standards ◽  
Chemiluminescence Immunoassay ◽  
Potential Signal ◽  
Acridinium Ester ◽  
Hydrogel Microspheres

Hydrogel microspheres sensitive to temperature as new potential signal enhancers and magnetic fluorescent nanoparticles as internal standards were used to establish a new CLIA method for the accurate diagnosis of cTnI in the human body.

Molecular Engineering of Hybrid Dye–Silica Fluorescent Nanoparticles: Influence of the Dye Structure on the Distribution of Fluorophores and Consequent Photoemission Brightness

2012 ◽  
Vol 24 (14) ◽  
pp. 2792-2801 ◽  
Author(s):  
Gabriele Alberto ◽  
Giuseppe Caputo ◽  
Guido Viscardi ◽  
Salvatore Coluccia ◽  
Gianmario Martra
Keyword(s):  
Molecular Engineering ◽  
Fluorescent Nanoparticles ◽  
Dye Structure

A simple and effective method to synthesize fluorescent nanoparticles using tryptophan and light and their lethal effect against bacteria

2014 ◽  
Vol 140 ◽  
pp. 157-162 ◽  
Author(s):  
Rafael Jun Tomita ◽  
Ricardo Almeida de Matos ◽  
Marcelo Afonso Vallim ◽  
Lilia Coronato Courrol
Keyword(s):  
Lethal Effect ◽  
Fluorescent Nanoparticles

Generic Method of Preparing Multifunctional Fluorescent Nanoparticles Using Flash NanoPrecipitation

2009 ◽  
Vol 19 (5) ◽  
pp. 718-725 ◽  
Author(s):  
Mustafa Akbulut ◽  
Paul Ginart ◽  
Marian E. Gindy ◽  
Christian Theriault ◽  
Katherine H. Chin ◽  
...  
Keyword(s):  
Fluorescent Nanoparticles ◽  
Flash Nanoprecipitation
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