Mueller matrix microscopy for label-free histopathology examinations (Conference Presentation)

2017 ◽  
Author(s):  
Donghong Lv ◽  
Honghui He ◽  
Jialing Zhou ◽  
Hui Ma
Keyword(s):  
Mueller Matrix ◽  
Label Free

Mueller Matrix Polarimetry: A Label-Free, Quantitative Optical Method for Clinical Diagnosis

2020 ◽  
Vol 47 (2) ◽  
pp. 0207001
Author(s):  
沈元星 Shen Yuanxing ◽  
姚悦 Yao Yue ◽  
何宏辉 He Honghui ◽  
刘少雄 Liu Shaoxiong ◽  
马辉 Ma Hui
Keyword(s):  
Clinical Diagnosis ◽  
Optical Method ◽  
Mueller Matrix ◽  
Label Free

scholarly journals Zebrafish structural development in Mueller-matrix scanning microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aymeric Le Gratiet ◽  
Marta d’Amora ◽  
Marti Duocastella ◽  
Riccardo Marongiu ◽  
Artemi Bendandi ◽  
...  
Keyword(s):  
Structural Changes ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Mueller Matrix ◽  
Fluorescence Probes ◽  
Imaging Method ◽  
Structural Development ◽  
Matrix Formalism ◽  
Label Free ◽  
Biological Organization

AbstractZebrafish are powerful animal models for understanding biological processes and the molecular mechanisms involved in different human diseases. Advanced optical techniques based on fluorescence microscopy have become the main imaging method to characterize the development of these organisms at the microscopic level. However, the need for fluorescence probes and the consequent high light doses required to excite fluorophores can affect the biological process under observation including modification of metabolic function or phototoxicity. Here, without using any labels, we propose an implementation of a Mueller-matrix polarimeter into a commercial optical scanning microscope to characterize the polarimetric transformation of zebrafish preserved at different embryonic developmental stages. By combining the full polarimetric measurements with statistical analysis of the Lu and Chipman mathematical decomposition, we demonstrate that it is possible to quantify the structural changes of the biological organization of fixed zebrafish embryos and larvae at the cellular scale. This convenient implementation, with low light intensity requirement and cheap price, coupled with the quantitative nature of Mueller-matrix formalism, can pave the way for a better understanding of developmental biology, in which label-free techniques become a standard tool to study organisms.

scholarly journals Polarization Label-Free Microscopy Imaging of Biological Samples by Exploiting the Zeeman Laser Emission

2021 ◽  
Vol 9 ◽  
Author(s):  
Fabio Callegari ◽  
Aymeric Le Gratiet ◽  
Alessandro Zunino ◽  
Ali Mohebi ◽  
Paolo Bianchini ◽  
...  
Keyword(s):  
Biological Samples ◽  
Mueller Matrix ◽  
Scanning Microscopy ◽  
Laser Source ◽  
Orthogonal Polarization ◽  
Interference Signal ◽  
Label Free ◽  
Microscopy Imaging ◽  
Polarization States ◽  
Zeeman Laser

Mueller Matrix Microscopy exploits the generation and the analysis of polarized light to create label-free contrast in biological images. However, when dealing with Optical Scanning Microscopy, it is required a fast generation of the polarization states in order to obtain a good Signal-to-Noise Ratio at the pixel-dwell time rate. In this work, we propose a microscopy system based on a scanning beam architecture that is exploiting the simultaneous emission of orthogonal polarization states from a Zeeman laser to provide Mueller Matrix images. This approach is based on the detection of an interference signal that allows to time-encode polarization states directly from the laser source, without the need for further active components for the management of the polarization states. We provide the theoretical model behind this approach and we apply our new method to the imaging of biological samples. Our Mueller Matrix imaging setup enables high-speed scanning microscopy, while preserving compactness and simplicity of construction. Our findings may lead to more effective dissemination of label-free techniques and their use by biological researchers.

scholarly journals Review on Complete Mueller Matrix Optical Scanning Microscopy Imaging

2021 ◽  
Vol 11 (4) ◽  
pp. 1632
Author(s):  
Aymeric Le Gratiet ◽  
Ali Mohebi ◽  
Fabio Callegari ◽  
Paolo Bianchini ◽  
Alberto Diaspro
Keyword(s):  
High Speed ◽  
Polarized Light ◽  
Mueller Matrix ◽  
Scanning Microscopy ◽  
Label Free ◽  
Microscopy Imaging ◽  
Excitation Light ◽  
Optical Scanning ◽  
Polarization Control ◽  
Main Challenge

Optical scanning microscopy techniques based on the polarization control of the light have the capability of providing non invasive label-free contrast. By comparing the polarization states of the excitation light with its transformation after interaction with the sample, the full optical properties can be summarized in a single 4×4 Mueller matrix. The main challenge of such a technique is to encode and decode the polarized light in an optimal way pixel-by-pixel and take into account the polarimetric artifacts from the optical devices composing the instrument in a rigorous calibration step. In this review, we describe the different approaches for implementing such a technique into an optical scanning microscope, that requires a high speed rate polarization control. Thus, we explore the recent advances in term of technology from the industrial to the medical applications.

scholarly journals Multimodal Label Free Stokes/Mueller Matrix and Non Linear Scanning Microscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 279a
Author(s):  
Aymeric Le Gratiet ◽  
Riccardo Marongiu ◽  
Paolo Bianchini ◽  
alberto diaspro
Keyword(s):  
Mueller Matrix ◽  
Scanning Microscopy ◽  
Label Free ◽  
Non Linear ◽  
Linear Scanning

Label-free discrimination of lung cancer cells through mueller matrix decomposition of diffuse reflectance imaging

2018 ◽  
Vol 40 ◽  
pp. 505-518 ◽  
Author(s):  
Suman Shrestha ◽  
Aditi Deshpande ◽  
Tannaz Farrahi ◽  
Thomas Cambria ◽  
Tri Quang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Diffuse Reflectance ◽  
Matrix Decomposition ◽  
Mueller Matrix ◽  
Lung Cancer Cells ◽  
Label Free ◽  
Diffuse Reflectance Imaging

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

Checking the Mueller matrix

1999 ◽  
Author(s):  
D. Look, Jr.
Keyword(s):  
Mueller Matrix
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