Combining Raman spectroscopy and digital holographic microscopy for label-free classification of human immune cells (Conference Presentation)

2017 ◽  
Author(s):  
Naomi McReynolds ◽  
Fiona G. M. Cooke ◽  
Mingzhou Chen ◽  
Simon J. Powis ◽  
Kishan Dholakia
Keyword(s):  
Raman Spectroscopy ◽  
Immune Cells ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy ◽  
Human Immune ◽  
Free Classification

scholarly journals High accuracy label-free classification of kinetic cell states from holographic cytometry

2017 ◽  
Author(s):  
Miroslav Hejna ◽  
Aparna Jorapur ◽  
Jun S. Song ◽  
Robert L. Judson
Keyword(s):  
Low Cost ◽  
Cancer Therapeutics ◽  
High Accuracy ◽  
Digital Holographic Microscopy ◽  
Adherent Cell ◽  
Label Free ◽  
Multiple Behaviors ◽  
Holographic Microscopy ◽  
Free Classification

AbstractDigital holographic microscopy permits live and label-free visualization of adherent cells. Here we report the application of this approach for high accuracy kinetic quantitative cytometry. We identify twenty-six label-free optical and morphological features that are biologically independent. When used as a basis for machine learning, these features allow blind single cell classification with up to 95% accuracy. We present methods to control for inherent holographic noise, thereby establishing a set of reliable quantitative features. Together, these contributions permit continuous digital holographic cytometry for three or more days. Applying our approach to human melanoma cells treated with a panel of cancer therapeutics, we can track the response of each cell, simultaneously classifying multiple behaviors such as cell cycle length, motility, apoptosis, senescence, and heterogeneity of response to each therapeutic. Importantly, we demonstrate relationships between these phenotypes over time. This work thus provides an experimental and computational roadmap for low cost live-cell imaging and kinetic classification of heterogeneous adherent cell populations.

scholarly journals Towards automated cancer screening: Label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy

2018 ◽  
Vol 11 (4) ◽  
pp. e201700244 ◽  
Author(s):  
Lana Woolford ◽  
Mingzhou Chen ◽  
Kishan Dholakia ◽  
C. Simon Herrington
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Screening ◽  
Label Free ◽  
Fixed Cell ◽  
Free Classification

scholarly journals Multimodal discrimination of immune cells using a combination of Raman spectroscopy and digital holographic microscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Naomi McReynolds ◽  
Fiona G. M. Cooke ◽  
Mingzhou Chen ◽  
Simon J. Powis ◽  
Kishan Dholakia
Keyword(s):  
Raman Spectroscopy ◽  
Immune Cells ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

scholarly journals Label-free fingerprinting of tumor cells in bulk flow using inline digital holographic microscopy

2017 ◽  
Vol 8 (2) ◽  
pp. 536 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Bulk Flow ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy

Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

2010 ◽  
Vol 35 (24) ◽  
pp. 4102 ◽  
Author(s):  
Etienne Shaffer ◽  
Corinne Moratal ◽  
Pierre Magistretti ◽  
Pierre Marquet ◽  
Christian Depeursinge
Keyword(s):  
Second Harmonic ◽  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Label Free ◽  
Biological Specimen ◽  
Harmonic Phase ◽  
Holographic Microscopy ◽  
Harmonic Phase Imaging
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