Optimizing ultrafast wide field-of-view illumination for high-throughput multi-photon imaging and screening of mutant fluorescent proteins

2017 ◽  
Author(s):  
Caleb Stoltzfus ◽  
Alexandr Mikhailov ◽  
Aleksander Rebane
Keyword(s):  
High Throughput ◽  
Fluorescent Proteins ◽  
Field Of View ◽  
Wide Field ◽  
Wide Field Of View ◽  
Photon Imaging

Lensless On-Chip Fluorescent Imaging Over an Ultra Wide Field-of-View

2010 ◽  
Author(s):  
Ahmet F. Coskun ◽  
Ting-Wei Su ◽  
Aydogan Ozcan
Keyword(s):  
High Throughput ◽  
Imaging Modality ◽  
Scattered Light ◽  
Fluorescent Imaging ◽  
Field Of View ◽  
Fluorescent Detection ◽  
Fluorescent Light ◽  
Wide Field ◽  
Excitation Light ◽  
Wide Field Of View

We introduce a lensless high-throughput fluorescent detection modality that can simultaneously image micro-objects and labeled cells over an ultra-wide field-of-view (FOV) of ∼8cm2 without the use of any lenses, thin-film filters and mechanical scanners. This lensfree platform utilizes total-internal-reflection (TIR) to block the excitation light, and an inexpensive absorption filter to remove the weakly scattered light that does not obey TIR. The emitted fluorescent light from the objects is then detected on the same chip without the use of any lenses. A digital deconvolution algorithm is used to resolve overlapping fluorescent spots, enabling a resolution of ∼40–50 μm over the entire field-of-view. Such an ultra wide field-of-view lensfree fluorescent imaging modality might be very valuable for high-throughput screening applications as well as quantification of rare cells such as circulating tumor cells using ultra-large microfluidic devices.

scholarly journals Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain

2016 ◽  
Vol 34 (8) ◽  
pp. 857-862 ◽  
Author(s):  
Jeffrey N Stirman ◽  
Ikuko T Smith ◽  
Michael W Kudenov ◽  
Spencer L Smith
Keyword(s):  
Neuronal Activity ◽  
Field Of View ◽  
Mammalian Brain ◽  
Wide Field ◽  
Two Photon ◽  
Wide Field Of View ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Protocol for cortical-wide field-of-view two-photon imaging with quick neonatal adeno-associated virus injection

2021 ◽  
Vol 2 (4) ◽  
pp. 101007
Author(s):  
Ikumi Oomoto ◽  
Hiroyuki Uwamori ◽  
Chie Matsubara ◽  
Maya Odagawa ◽  
Midori Kobayashi ◽  
...  
Keyword(s):  
Field Of View ◽  
Wide Field ◽  
Adeno Associated Virus ◽  
Two Photon ◽  
Wide Field Of View ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms

2010 ◽  
Vol 18 (7) ◽  
pp. 7203 ◽  
Author(s):  
Takeo Ejima ◽  
Fumihiko Ishida ◽  
Hiromichi Murata ◽  
Mitsunori Toyoda ◽  
Tetsuo Harada ◽  
...  
Keyword(s):  
High Throughput ◽  
Field Of View ◽  
Wide Field ◽  
Wide Field Of View ◽  
Living Organisms

scholarly journals Wide field-of-view, twin-region two-photon imaging across extended cortical networks

2014 ◽  
Author(s):  
Jeffrey N. Stirman ◽  
Ikuko T. Smith ◽  
Michael W. Kudenov ◽  
Spencer L. Smith
Keyword(s):  
Imaging System ◽  
Field Of View ◽  
Wide Field ◽  
Cortical Networks ◽  
Two Photon ◽  
Cortical Areas ◽  
Cellular Resolution ◽  
Wide Field Of View ◽  
Photon Imaging ◽  
Two Photon Imaging

We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm^2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.

STM studies of crystal growth

1991 ◽  
Vol 49 ◽  
pp. 374-375
Author(s):  
M. G. Lagally
Keyword(s):  
Crystal Growth ◽  
Molecular Beam ◽  
Chemical Vapor ◽  
Sputter Deposition ◽  
Field Of View ◽  
Scanning Tunneling ◽  
Wide Field ◽  
Tunneling Microscopy ◽  
Wide Field Of View ◽  
The One

It has been recognized since the earliest days of crystal growth that kinetic processes of all Kinds control the nature of the growth. As the technology of crystal growth has become ever more refined, with the advent of such atomistic processes as molecular beam epitaxy, chemical vapor deposition, sputter deposition, and plasma enhanced techniques for the creation of “crystals” as little as one or a few atomic layers thick, multilayer structures, and novel materials combinations, the need to understand the mechanisms controlling the growth process is becoming more critical. Unfortunately, available techniques have not lent themselves well to obtaining a truly microscopic picture of such processes. Because of its atomic resolution on the one hand, and the achievable wide field of view on the other (of the order of micrometers) scanning tunneling microscopy (STM) gives us this opportunity. In this talk, we briefly review the types of growth kinetics measurements that can be made using STM. The use of STM for studies of kinetics is one of the more recent applications of what is itself still a very young field.

Design and verification of high-precision multi-star simulator with a wide field of view

2020 ◽  
Vol 13 (6) ◽  
pp. 1-9
Author(s):  
XU Hong-gang ◽  
◽  
HAN Bing ◽  
LI Man-li ◽  
MA Hong-tao ◽  
...  
Keyword(s):  
High Precision ◽  
Field Of View ◽  
Wide Field ◽  
Wide Field Of View
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