Azimuthally invariant Mueller-matrix mapping of optically anisotropic layers of biological networks of blood plasma in the diagnosis of liver disease

2016 ◽  
Author(s):  
A. G. Ushenko ◽  
A. V. Dubolazov ◽  
V. A. Ushenko ◽  
Yu. A. Ushenko ◽  
M. Y. Sakhnovskiy ◽  
...  
Keyword(s):  
Liver Disease ◽  
Blood Plasma ◽  
Biological Networks ◽  
Mueller Matrix ◽  
Matrix Mapping ◽  
Anisotropic Layers

scholarly journals Polarization-Singular Approach to Imaging Mueller-Matrix Polarimetry in the Differential Diagnosis of Histological Sections of Biopsy of Tumors of the Uterus and Prostate

2021 ◽  
Vol 9 ◽  
Author(s):  
A. Dubolazov ◽  
V. Ushenko ◽  
L. Trifonyuk ◽  
A. Stashkevich ◽  
I. Soltys ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Mueller Matrix ◽  
Evidence Based ◽  
Malignant Tumours ◽  
Diagnostic Use ◽  
Matrix Mapping ◽  
Adenocarcinoma Of The Prostate ◽  
Characteristic Values ◽  
Sensitivity Specificity ◽  
Based Medicine

The possibilities of the diagnostic use of the singular approach of the distributions of the number of characteristic values of the MMI is effective for differentiating the polarization properties of histological biopsy sections of benign and malignant tumours of the uterus and prostate. Within the framework of evidence-based medicine, the sensitivity, specificity and accuracy of the azimuthal-invariant express (∼15 min) method of Mueller-matrix mapping of polarization-singular states in the differential diagnosis of uterine myoma and adenocarcinoma, as well as adenocarcinoma of the prostate with varying degrees of differentiation have been determined.

Mueller-matrix mapping of biological tissues in differential diagnosis of optical anisotropy mechanisms of protein networks

2015 ◽  
Vol 45 (3) ◽  
pp. 265-269 ◽  
Author(s):  
V A Ushenko ◽  
M I Sidor ◽  
Yu F Marchuk ◽  
N V Pashkovskaya ◽  
D R Andreichuk
Keyword(s):  
Differential Diagnosis ◽  
Optical Anisotropy ◽  
Biological Tissues ◽  
Mueller Matrix ◽  
Protein Networks ◽  
Matrix Mapping

scholarly journals Differential Mueller matrix imaging of partially depolarizing optically anisotropic biological tissues

2019 ◽  
Vol 35 (4) ◽  
pp. 877-891 ◽  
Author(s):  
L. Trifonyuk ◽  
A. Sdobnov ◽  
W. Baranowski ◽  
V. Ushenko ◽  
O. Olar ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Mueller Matrix ◽  
Differential Diagnostics ◽  
Matrix Elements ◽  
Polycrystalline Structure ◽  
First Order ◽  
Histological Sections ◽  
Laser Polarimetry ◽  
Matrix Mapping ◽  
Differential Matrix

AbstractSince recently, a number of innovative polarization-based optical imaging modalities have been introduced and extensively used in various biomedical applications, with an ultimate aim to attain the practical tool for the optical biopsy and functional characterization of biological tissues. The techniques utilize polarization properties of light and Mueller matrix mapping of microscopic images of histological sections of biological tissues or polycrystalline films of biological fluids. The main drawback of currently developed laser polarimetry approaches and Mueller matrix mapping techniques is poor reproducibility of experimental data. This is due to azimuthal dependence of polarization and ellipticity values of most matrix elements to sample orientation in respect to incidence light polarization. Current study aims to generalize the methods of laser polarimetry for diagnosis of partially depolarizing optically anisotropic biological tissues. A method of differential Mueller matrix mapping for reconstruction of linear and circular birefringence and dichroism parameter distributions of partially depolarizing layers of biological tissues of different morphological structure is introduced and practically implemented. The coordinate distributions of the value of the first-order differential matrix elements of histological sections of brain tissue with spatially structured, optically anisotropic fibrillar network, as well as of parenchymatous tissue of the rectum wall with an “islet” polycrystalline structure are determined. Within the statistical analysis of polarization reproduced distributions of the averaged parameters of phase and amplitude anisotropy, the significant sensitivity of the statistical moments of the third and fourth orders to changes in the polycrystalline structure of partially depolarizing layers of biological tissue is observed. The differentiation of female reproductive sphere connective tissue is realized with excellent accuracy. The differential Mueller matrix mapping method for reconstruction of distributions of linear and circular birefringence and dichroism parameters of partially depolarizing layers of biological tissues of different morphological structures is proposed and substantiated. Differential diagnostics of changes in the phase (good balanced accuracy) and amplitude (excellent balanced accuracy) of the anisotropy of the partially depolarizing layers of the vagina wall tissue with prolapse of the genitals is realized. The maximum diagnostic efficiency of the first-order differential matrix method was demonstrated in comparison with the traditional methods of polarization and Mueller matrix mapping of histological sections of light-scattering biological tissues.

scholarly journals 3D Mueller matrix mapping of layered distributions of depolarisation degree for analysis of prostate adenoma and carcinoma diffuse tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Volodymyr A. Ushenko ◽  
Benjamin T. Hogan ◽  
Alexander Dubolazov ◽  
Gennadii Piavchenko ◽  
Sergey L. Kuznetsov ◽  
...  
Keyword(s):  
Gleason Score ◽  
Mueller Matrix ◽  
Low Grade ◽  
High Grade ◽  
Tissue Samples ◽  
Prostate Tumour ◽  
Carcinoma Tissue ◽  
Matrix Mapping ◽  
Prostate Adenoma ◽  
Malignant Prostate

AbstractProstate cancer is the second most common cancer globally in men, and in some countries is now the most diagnosed form of cancer. It is necessary to differentiate between benign and malignant prostate conditions to give accurate diagnoses. We aim to demonstrate the use of a 3D Mueller matrix method to allow quick and easy clinical differentiation between prostate adenoma and carcinoma tissues with different grades and Gleason scores. Histological sections of benign and malignant prostate tumours, obtained by radical prostatectomy, were investigated. We map the degree of depolarisation in the different prostate tumour tissues using a Mueller matrix polarimeter set-up, based on the superposition of a reference laser beam with the interference pattern of the sample in the image plane. The depolarisation distributions can be directly related to the morphology of the biological tissues. The dependences of the magnitude of the 1st to 4th order statistical moments of the depolarisation distribution are determined, which characterise the distributions of the depolarisation values. To determine the diagnostic potential of the method three groups of histological sections of prostate tumour biopsies were formed. The first group contained 36 adenoma tissue samples, while the second contained 36 carcinoma tissue samples of a high grade (grade 4: poorly differentiated—4 + 4 Gleason score), and the third group contained 36 carcinoma tissue samples of a low grade (grade 1: moderately differentiated—3 + 3 Gleason score). Using the calculated values of the statistical moments, tumour tissues are categorised as either adenoma or carcinoma. A high level (> 90%) accuracy of differentiation between adenoma and carcinoma samples was achieved for each group. Differentiation between the high-grade and low-grade carcinoma samples was achieved with an accuracy of 87.5%. The results demonstrate that Mueller matrix mapping of the depolarisation distribution of prostate tumour tissues can accurately differentiate between adenoma and carcinoma, and between different grades of carcinoma. This represents a first step towards the implementation of 3D Mueller matrix mapping for clinical analysis and diagnosis of prostate tumours.

scholarly journals POLARIZATION TOMOGRAPHY METHODS TO DIFFERENTIATE ASEPTIC AND SEPTIC LOOSENING AFTER TOTAL HIP REPLACEMENT

2020 ◽  
pp. 33-46
Author(s):  
V.L. Vasyuk ◽  
V.V. Protsiuk ◽  
Y.M. Vasilchishin ◽  
S.V. Vasiuk
Keyword(s):  
Total Hip Replacement ◽  
Hip Replacement ◽  
Mueller Matrix ◽  
Mapping Method ◽  
Control Group ◽  
Septic Loosening ◽  
Logical Scheme ◽  
Total Hip ◽  
Polarization Tomography ◽  
Matrix Mapping

Abstract. This article contains: • Structural-logical scheme and analytical description of the differential diagnosis for aseptic and septic loosening after total hip replacement using the methods of differential Mueller-matrix mapping of circular birefringence (CB) distributions of polycrystalline synovial fluid (SF) films. • Statistical analysis of the distributions of CB in polycrystalline SF films obtained from patients in the control group and groups with different hip joint pathology. • Results of establishing the strength of the differential Mueller-matrix mapping method of the distributions of CB polycrystalline films of SF by means of information analysis based on sensitivity determination, specificity and accuracy of the polarization tomography technique. • Clinical example of using polarization tomography methods to differentiate aseptic and septic cup loosening after total hip replacement

scholarly journals Time since hemorrhages of traumatic and non-traumatic genesis into the substance of the human brain formation using distribution of linear dichroism fluctuations reconstruction

2021 ◽  
pp. 25-35
Author(s):  
Marta Garazdiuk
Keyword(s):  
Human Brain ◽  
Cause Of Death ◽  
Temporal Dynamics ◽  
Statistical Processing ◽  
Mueller Matrix ◽  
Statistical Moments ◽  
Histological Sections ◽  
Forensic Practice ◽  
Matrix Mapping ◽  
The Brain

For a forensic expert-practitioner, it is especially important to objectively diagnose and time since the formation of hemorrhage (TSFH) in the substance of the human brain (SHB) of traumatic and non-traumatic origin, as there are cases when the external examination of the corpse at the scene are absent, and at internal research find hemorrhages in a brain. In forensic practice, to verify the cause of death, physical-optical methods are successfully used, which are based on laser irradiation of biological tissues with subsequent mathematical and statistical processing of the obtained data. Previous studies on the possibility of differentiating the cause of death by traditional polarization methods have yielded positive results, which suggests the possibility of their suitability for verification of the genesis of hemorrhage into the brain. For a forensic expert-practitioner, the main thing is objectivity, accuracy and speed of obtaining the result, which could fully satisfy the methods of laser polarimetry in the case of determining the TSFH of traumatic and non-traumatic origin in SHB. Therefore, it is necessary to continue the development and research of these methods for this purpose. Aim of the work. To substantiate the possibility of using the method of differential Mueller-matrix mapping of phase anisotropy to determine the temporal dynamics of maps of linear birefringence of histological sections of human brain in determining the age of hemorrhage in human brain substance and to develop forensic criteria for determining the age. death due to cerebral infarction of ischemic and hemorrhagic origin. Materials and methods. To achieve this goal, we studied native histological preparations SHB from 130 corpses with a known time of death. The cause of death was TBI (group II (n=35)), cerebral infarction of ischemic origin (group III (n=32)), hemorrhagic stroke (group IV (n=33)), acute coronary insufficiency (group I – comparison group (n=30)). The values of the distribution of the coordinates of the polarization parameters at the points of the microscopic images at the location of the standard Stokes polarimeter were measured. Experimental measurements of Stokes-parametric images of biological layers were performed according to the method presented in the sources. Subsequently, the obtained data were subjected to statistical processing and evaluation of the obtained results. Statistical moments (SM) of the 1st-4th orders (mean (SM1), variance (SM2), asymmetry (SM3) and excess (SM4)) of each map were determined. Results and discussion. Comparative analysis of polarization Mueller-matrix mapping images of SHB sections from all groups revealed the destruction of the polycrystalline structure formed by optically active protein complexes of the brain substance, which indicates a decrease in absolute values and range of their scatter with increasing hemorrhage time. This is indicated by the coordinate inhomogeneity of the Mueller-matrix invariant maps of histological sections of SHB of all groups. For histograms that characterize the distributions of the Mueller-matrix invariant samples from all (comparison groups 1 and experimental 2-4) groups, are characterized by individual and significant variations in the values of statistical moments. Due to this, with increasing hemorrhage time, the value of the mean (SM1) and variance (SM2) decreases. Asymmetry (SM3) and excess (SM4), on the contrary, increase. The analysis of the results of statistical processing of the topographic structure of LD tomograms of fibrillar networks of histological sections of SHB dead from all groups shows a greater temporal dynamics of necrotic destruction of nervous tissue. Accordingly, there is a faster time decrease in the absolute values and the range of scatter of the LD value with increasing TSFH. That is, the diagnostic sensitivity of the statistical moments of the 3rd and 4th orders for azimuthal-invariant Mueller-matrix differentiation of nerve tissue samples of the brain of the deceased of control group 1 and all experimental groups 2-4 (p<0,05) was revealed. Conclusions. A series of studies of the effectiveness of a new in forensic practice method of differential Mueller-matrix mapping of partially depolarizing histological sections of SHB and tomographic reproduction of optical anisotropy parameters of their polycrystalline structure revealed a high level of accuracy of differentiation and formation of genesis, even under conditions of small geometric thickness of experimental samples. The range of linear change of values of statistical moments of the 1st - 4th orders which characterize distributions of size of LD of fibrillar networks of histologic sections of SHB of the dead from all groups, makes 24 h. In the range of 6-24 hours, the accuracy of determining the TSFH using statistical processing of the topographic structure of LD tomograms of fibrillar networks of histological sections of TSFH is (30±5) minutes.

scholarly journals 3D Mueller-matrix mapping of biological optically anisotropic networks

2018 ◽  
Author(s):  
Alexander Ushenko ◽  
Vladimir Ushenko ◽  
Inna Lukashevich ◽  
Galina Bodnar ◽  
Viktor Zhytaryuk ◽  
...  
Keyword(s):  
Mueller Matrix ◽  
Matrix Mapping ◽  
Anisotropic Networks
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