Built-in hyperspectral camera for smartphone in visible, near-infrared and middle-infrared lights region (third report): spectroscopic imaging for broad-area and real-time componential analysis system against local unexpected terrorism and disasters

2016 ◽  
Author(s):  
Satsuki Hosono ◽  
Natsumi Kawashima ◽  
Dirk Wollherr ◽  
Ichiro Ishimaru
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Spectroscopic Imaging ◽  
Broad Area ◽  
Componential Analysis ◽  
Analysis System ◽  
Middle Infrared ◽  
Hyperspectral Camera

scholarly journals Analysis of Video Images Using an Interactive Image Capture and Analysis System

HortScience ◽  
1990 ◽  
Vol 25 (6) ◽  
pp. 695-697 ◽  
Author(s):  
G.W. Stutte
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Physiological Status ◽  
Interactive Software ◽  
Image Capture ◽  
Video Images ◽  
Scientific Agriculture ◽  
Input Source ◽  
Analysis System ◽  
Video Input

Interactive Image Capture and Analysis System (ICAS) provides for real-time capture of video images using an imaging board and software in a personal computer. Through the use of selective filters on the video input source, images of specific reflective wavelengths are obtained and then analyzed for intensity distribution using interactive software designed for scientific agriculture. Conversion of video cameras into two-dimensional near real-time visual and near infrared (NIR) spectral sensors through the use of filters provides information on the physiological status of the tissue following ICAS analysis. However, caution must be observed to minimize equipment-induced artifacts during image acquisition and analysis.

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

Real-time tracking lysosomal pH changes under heatstroke and redox stress with a novel near-infrared emissive probe

Talanta ◽  
2021 ◽  
Vol 228 ◽  
pp. 122184
Author(s):  
Qingfeng Xia ◽  
Shumin Feng ◽  
Jiaxin Hong ◽  
Guoqiang Feng
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Redox Stress ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Real Time Tracking ◽  
Emissive Probe
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