Two-photon autofluorescence/FLIM/SHG endoscopy to study the oral cavity and wound healing in humans (Conference Presentation)

2016 ◽  
Author(s):  
Karsten König
Keyword(s):  
Wound Healing ◽  
Oral Cavity ◽  
Two Photon

scholarly journals Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Markus Seeger ◽  
Christoph Dehner ◽  
Dominik Jüstel ◽  
Vasilis Ntziachristos
Keyword(s):  
Wound Healing ◽  
Label Free ◽  
Third Harmonic ◽  
Two Photon ◽  
Non Invasive ◽  
Photon Excitation ◽  
Free Mode ◽  
Spatio Temporal ◽  
And Function

AbstractThe non-invasive investigation of multiple biological processes remains a methodological challenge as it requires capturing different contrast mechanisms, usually not available with any single modality. Intravital microscopy has played a key role in dynamically studying biological morphology and function, but it is generally limited to resolving a small number of contrasts, typically generated by the use of transgenic labels, disturbing the biological system. We introduce concurrent 5-modal microscopy (Co5M), illustrating a new concept for label-free in vivo observations by simultaneously capturing optoacoustic, two-photon excitation fluorescence, second and third harmonic generation, and brightfield contrast. We apply Co5M to non-invasively visualize multiple wound healing biomarkers and quantitatively monitor a number of processes and features, including longitudinal changes in wound shape, microvascular and collagen density, vessel size and fractality, and the plasticity of sebaceous glands. Analysis of these parameters offers unique insights into the interplay of wound closure, vasodilation, angiogenesis, skin contracture, and epithelial reformation in space and time, inaccessible by other methods. Co5M challenges the conventional concept of biological observation by yielding multiple simultaneous parameters of pathophysiological processes in a label-free mode.

Two-Photon Fluorescence Microscopy Deep Vascular Imaging of a Wound Healing Model

2013 ◽  
Author(s):  
Ciceron O. Yanez ◽  
Alma R. Morales ◽  
Xiling Yue ◽  
Takeo Urakami ◽  
Masanobu Komatsu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Fluorescence Microscopy ◽  
Vascular Imaging ◽  
Two Photon ◽  
Wound Healing Model ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Healing Model

scholarly journals Smoking Related Systemic and Oral Diseases

2007 ◽  
Vol 50 (3) ◽  
pp. 161-166 ◽  
Author(s):  
Sajith Vellappally ◽  
Zdeněk Fiala ◽  
Jindra Šmejkalová ◽  
Vimal Jacob ◽  
Rakesh Somanathan
Keyword(s):  
Lung Cancer ◽  
Wound Healing ◽  
Survival Rate ◽  
Oral Cavity ◽  
Oral Candidiasis ◽  
Systemic Diseases ◽  
Oral Diseases ◽  
Implant Survival ◽  
Dental Restorations ◽  
The Relationship

This article reviewed smoking related systemic diseases and oral diseases. Smoking is related to lung cancer, cardiovascular diseases and many other systemic diseases. Cigarette smoke affects the oral cavity first, so it is evident that smoking has many negative influences on oral cavity, for example, staining of teeth and dental restorations, wound healing, reduction of the ability to smell and taste, and development of oral diseases such as oral cancer, periodontitis, smoker’s palate, smoker’s melanosis, hairy tongue, leukoplakia, oral candidiasis and implant survival rate. The article also discusses the relationship between smoking and dental caries in detail.

Effects of fibrin glue on wound healing in oral cavity

2003 ◽  
Vol 31 (8) ◽  
pp. 569-575 ◽  
Author(s):  
Emre A Yücel ◽  
Okhan Oral ◽  
Vakur Olgaç ◽  
Cüneyt K Oral
Keyword(s):  
Wound Healing ◽  
Oral Cavity ◽  
Fibrin Glue

scholarly journals SALIVA AS A POTENTIAL DIAGNOSTIC OF BIOMARKER OF ORAL AND SYSTEMIC DISEASES

2016 ◽  
Vol 19 (2) ◽  
Author(s):  
Dian Lesmana ◽  
Sri Tjahajawati ◽  
Vita Tarawan Lubis
Keyword(s):  
Wound Healing ◽  
Oral Cavity ◽  
Biological Fluid ◽  
Systemic Disease ◽  
Physiological Status ◽  
Non Invasive ◽  
Food Particles ◽  
Psychological Influence ◽  
Cost Efficient ◽  
Invasive Techniques

Saliva is a biological fluid that is secreted by the major, minor and other accessories salivary glands. Saliva has a high potential as an indicator that reflects the health of our body. Saliva also plays an important role as a protector, lubricate oral structure with mucin, set a neutral pH through the buffer capacity, clean the oral cavity, stimulate wound healing, help the taste buds in the taste of food, facilitate the process of chewing food, form a bolus and make it easier swallowing, and minimize food particles through the activity of the enzyme amylase and lipase. The use of saliva as a biomarker in diagnosing provide more advantages than the serum and other body fluids due to using non-invasive techniques, cost-efficient, as well as methods for collecting samples for disease screening without the need to train professional personnel. The benefits derived from the saliva as a biomarker are early detection and screening of disease to the development of caries, periodontal disease, tumors and malignancy, hereditary, wound healing, autoimmune disease, infectious diseases, cardiovascular diseases, drug monitoring, forensic, and the psychological influence. At this time, the function of saliva as a diagnostic biomarker is not widely known, therefore the development of salivary further enhance because it can produce functional similarities with serum and reflect the body's physiological status in the oral cavity and systemic disease.  

scholarly journals Hemostasia in the Oral Cavity

1977 ◽  
Author(s):  
D. Tomic
Keyword(s):  
Wound Healing ◽  
Oral Cavity ◽  
Physical Properties ◽  
Bone Cement ◽  
Blood Coagulation ◽  
Organic Compounds ◽  
Ph Value ◽  
Additional Treatment ◽  
Special Method ◽  
Osmotic Properties

Various factors and reasons can be responsible for acute, severe or protracted hemorrhages in the oral cavity. Hemostasis with conventional methods frequently poses a difficult, dangerous and involved problem. A special method is developed utilizing the chemical and physical properties of inorganic and organic compounds, and this is found to be efficient for rapid and safe hemostasis. It acts on the blood coagulation process by lowering the pH value of blood, as well as by its astringent and osmotic properties. Altogether, the result is hemostasis and favorable wound healing. With the use of bone cement, the initial clot is physically protected, thus contributing more to reliable hemostasis, particularly after dental extraction. The method used, that is, bone cement in combination with the developed hemostatic agent, can be used without additional treatment for severe primary, as well as secondary hemorrhages. Patients suffering from hemophilia and other disorders of blood coagulation were treated successfully.

Wound healing after tonsillectomy – a review of the literature

2018 ◽  
Vol 132 (9) ◽  
pp. 764-770 ◽  
Author(s):  
N H Davidoss ◽  
R Eikelboom ◽  
P L Friedland ◽  
P L Santa Maria
Keyword(s):  
Wound Healing ◽  
Oral Cavity ◽  
English Literature ◽  
Healing Process ◽  
Experimental Models ◽  
Adjunctive Treatment ◽  
Review Of The Literature ◽  
Ovid Medline ◽  
Search Terms ◽  
Post Operative Period

AbstractObjectiveTo summarise the available literature related to wound healing post tonsillectomy, including the stages of healing, experimental models for assessing healing (in animals and humans) and the various factors that affect wound healing.MethodsA search of the English literature was conducted using the Ovid Medline database, with the search terms ‘tonsillectomy’ or ‘tonsil’ and ‘wound healing’. Thirty-one articles that objectively assessed tonsillectomy wound healing were included for analysis.ResultsThe majority of assessments in humans investigating tonsillectomy wound healing involve serial direct clinical examinations of the oral cavity. Many patient and surgical factors have been shown to affect wound healing after tonsillectomy. There is some research to suggest that the administration of adjunctive treatment in the post-operative period may be beneficial to tonsillectomy wound healing.ConclusionWound healing post tonsillectomy has been poorly researched. Having a better understanding of the process of wound healing would allow surgeons to potentially prevent, anticipate and manage complications from the surgery that arise as part of the healing process.

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