Using quantitative interference phase microscopy for sperm acrosome evaluation (Conference Presentation)

2016 ◽  
Author(s):  
Michal Balberg ◽  
Ksawery Kalinowski ◽  
Mattan Levi ◽  
Natan T. Shaked
Keyword(s):  
Phase Microscopy ◽  
Interference Phase ◽  
Sperm Acrosome

scholarly journals Single-shot two-frame π-shifted spatially multiplexed interference phase microscopy

2019 ◽  
Vol 24 (09) ◽  
pp. 1 ◽  
Author(s):  
Maciej Trusiak ◽  
Jose-Angel Picazo-Bueno ◽  
Krzysztof Patorski ◽  
Piotr Zdankowski ◽  
Vicente Mico
Keyword(s):  
Single Shot ◽  
Phase Microscopy ◽  
Interference Phase

scholarly journals Characterization of Liposomes Using Quantitative Phase Microscopy (QPM)

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Jennifer Cauzzo ◽  
Nikhil Jayakumar ◽  
Balpreet Singh Ahluwalia ◽  
Azeem Ahmad ◽  
Nataša Škalko-Basnet
Keyword(s):  
Rapid Development ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Batch Mode ◽  
Full Field ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Tracking Ability

The rapid development of nanomedicine and drug delivery systems calls for new and effective characterization techniques that can accurately characterize both the properties and the behavior of nanosystems. Standard methods such as dynamic light scattering (DLS) and fluorescent-based assays present challenges in terms of system’s instability, machine sensitivity, and loss of tracking ability, among others. In this study, we explore some of the downsides of batch-mode analyses and fluorescent labeling, while introducing quantitative phase microscopy (QPM) as a label-free complimentary characterization technique. Liposomes were used as a model nanocarrier for their therapeutic relevance and structural versatility. A successful immobilization of liposomes in a non-dried setup allowed for static imaging conditions in an off-axis phase microscope. Image reconstruction was then performed with a phase-shifting algorithm providing high spatial resolution. Our results show the potential of QPM to localize subdiffraction-limited liposomes, estimate their size, and track their integrity over time. Moreover, QPM full-field-of-view images enable the estimation of a single-particle-based size distribution, providing an alternative to the batch mode approach. QPM thus overcomes some of the drawbacks of the conventional methods, serving as a relevant complimentary technique in the characterization of nanosystems.

scholarly journals Magnetic textures in a hexaferrite thin film and their response to magnetic fields revealed by phase microscopy

2019 ◽  
Vol 58 (6) ◽  
pp. 065004 ◽  
Author(s):  
Atsuhiro Kotani ◽  
Ken Harada ◽  
Marek Malac ◽  
Hiroshi Nakajima ◽  
Kosuke Kurushima ◽  
...  
Keyword(s):  
Thin Film ◽  
Magnetic Fields ◽  
Phase Microscopy

scholarly journals Efficient quantitative phase microscopy using programmable annular LED illumination

2017 ◽  
Vol 8 (10) ◽  
pp. 4687 ◽  
Author(s):  
Jiaji Li ◽  
Qian Chen ◽  
Jialin Zhang ◽  
Yan Zhang ◽  
Linpeng Lu ◽  
...  
Keyword(s):  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy

Tomographic phase microscopy using optical tweezers

2015 ◽  
Author(s):  
Mor Habaza ◽  
Barak Gilboa ◽  
Yael Roichman ◽  
Natan T. Shaked
Keyword(s):  
Optical Tweezers ◽  
Phase Microscopy
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