Analysis of cancer cell morphology in fluorescence microscopy image exploiting shape descriptor

2016 ◽  
Author(s):  
Mi-Sun Kang ◽  
Hye-Ryun Kim ◽  
Sudong Kim ◽  
Gyu Ha Ryu ◽  
Myoung-Hee Kim
Keyword(s):  
Fluorescence Microscopy ◽  
Cancer Cell ◽  
Cell Morphology ◽  
Shape Descriptor ◽  
Microscopy Image

Initial Attachment of MC3T3-E1 Cells on Nano-HAp Surfaces

2007 ◽  
Vol 361-363 ◽  
pp. 1115-1118
Author(s):  
Un Hye Kwon ◽  
Jung Suk Han ◽  
In Young Ryu ◽  
Dae Joon Kim
Keyword(s):  
Surface Roughness ◽  
Cell Adhesion ◽  
Fluorescence Microscopy ◽  
Cell Morphology ◽  
Confocal Laser ◽  
Initial Attachment ◽  
Zirconia Ceramics ◽  
Initial Cell ◽  
Cell Adhesion Proteins ◽  
Initial Cell Adhesion

The initial osteoblast like cell response to bioactive nano-sized hydroxyapatite (HAp) and bioinert zirconia was evaluated with the cell morphology by SEM and cell adhesion proteins by fluorescence microscopy. Surface roughness also measured by a confocal laser microscopy. The surface roughness and topography was almost identical among specimens. The nano-sized HAp specimens showed better initial cell adhesion and activity than bioinert zirconia ceramics.

scholarly journals Differential Kras V12 protein levels control a switch regulating lung cancer cell morphology and motility

2016 ◽  
Vol 2 (3) ◽  
pp. 035004 ◽  
Author(s):  
C Schäfer ◽  
A Mohan ◽  
W Burford ◽  
M K Driscoll ◽  
A T Ludlow ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cell ◽  
Cell Morphology ◽  
Lung Cancer Cell ◽  
Protein Levels

scholarly journals Auto-Align - Multi-Modality Fluorescence Microscopy Image Co-registration

Traffic ◽  
2011 ◽  
Vol 13 (2) ◽  
pp. 204-217 ◽  
Author(s):  
William T. E. Pitkeathly ◽  
Natalie S. Poulter ◽  
Ela Claridge ◽  
Joshua Z. Rappoport
Keyword(s):  
Fluorescence Microscopy ◽  
Microscopy Image

Effect of AEE788 and/or Celecoxib on colon cancer cell morphology using advanced microscopic techniques

Micron ◽  
2010 ◽  
Vol 41 (3) ◽  
pp. 247-256 ◽  
Author(s):  
P. Venkatesan ◽  
Soumen Das ◽  
M. Muthu Rama Krishnan ◽  
Chandan Chakraborty ◽  
Koel Chaudhury ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cell ◽  
Cell Morphology ◽  
Colon Cancer Cell ◽  
Microscopic Techniques

Sperm Tracking and Trajectory Analysis in Fluorescence Microscopy Image Sequences

2021 ◽  
Author(s):  
Lucía Arboleya ◽  
Leonardo Santos ◽  
Mariano Fernández ◽  
Lucía Rosa-Villagrán ◽  
Rossana Sapiro ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Trajectory Analysis ◽  
Image Sequences ◽  
Microscopy Image

scholarly journals The Antiproliferative and Apoptotic Effects of Capsaicin on an Oral Squamous Cancer Cell Line of Asian Origin, ORL-48

Medicina ◽  
2019 ◽  
Vol 55 (7) ◽  
pp. 322 ◽  
Author(s):  
Mohammad Firdaus Kamaruddin ◽  
Mohammad Zakir Hossain ◽  
Aied Mohamed Alabsi ◽  
Marina Mohd Bakri
Keyword(s):  
Cell Cycle ◽  
Membrane Potential ◽  
Oral Cancer ◽  
Fluorescence Microscopy ◽  
Cell Line ◽  
Cancer Cell ◽  
Mitochondrial Membrane ◽  
Cancer Cell Line ◽  
Asian Origin ◽  
Cycle Arrest

Background and Objectives: The antitumor activities of capsaicin on various types of cancer cell lines have been reported but the effect of capsaicin on oral cancer, which is prevalent among Asians, are very limited. Thus, this study aimed to investigate the effects of capsaicin on ORL-48, an oral cancer cell line of Asian origin. Materials and Methods: Morphological changes of the ORL-48 cells treated with capsaicin were analyzed using fluorescence microscopy. The apoptotic-inducing activity of capsaicin was further confirmed by Annexin V-Fluorescein isothiocyanate / Propidium iodide (V-FITC/PI) staining using flow cytometry. In order to establish the pathway of apoptosis triggered by the compound on ORL-48 cells, caspase activity was determined and the mitochondrial pathway was verified by mitochondrial membrane potential (MMP) assay. Cell cycle analysis was also performed to identify the cell cycle phase of ORL-48 cells being inhibited by the capsaicin compound. Results: Fluorescence microscopy exhibited the presence of apoptotic features in capsaicin-treated ORL-48 cells. Apoptosis of capsaicin-treated ORL-48 cells revealed disruption of the mitochondrial-membrane potential, activation of caspase-3, -7 and -9 through an intrinsic apoptotic pathway and subsequently, apoptotic DNA fragmentation. The cell cycle arrest occurred in the G1-phase, confirming antiproliferative effect of capsaicin in a time-dependent manner. Conclusion: This study demonstrated that capsaicin is cytotoxic against ORL-48 cells and induces apoptosis in ORL-48 cells possibly through mitochondria mediated intrinsic pathway resulting in cell cycle arrest.

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