Quantification of neurotoxic effects on individual neuron cells using optical diffraction tomography (Conference Presentation)

2016 ◽  
Author(s):  
Jonghee Yoon ◽  
Su-a Yang ◽  
Kyoohyun Kim ◽  
YongKeun Park
Keyword(s):  
Optical Diffraction ◽  
Individual Neuron ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography ◽  
Neurotoxic Effects

scholarly journals Measurements of morphological and biochemical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson’s disease

2016 ◽  
Author(s):  
Su-A Yang ◽  
Jonghee Yoon ◽  
Kyoohyun Kim ◽  
YongKeun Park
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Optical Diffraction ◽  
Optical Measurements ◽  
Diffraction Tomography ◽  
Biochemical Alterations ◽  
Optical Diffraction Tomography ◽  
Neurotoxic Effects ◽  
Index Distribution

AbstractParkinson’s disease (PD) is a common neurodegenerative disease. However, therapeutic methods of PD are still limited due to complex pathophysiology in PD. Here, we present optical measurements of individual neurons fromin vitroPD model using optical diffraction tomography (ODT). By measuring 3-D refractive index distribution of neurons, morphological and biochemical alterations inin-vitroPD model are quantitatively investigated. We found that neurons show apoptotic features in early PD progression. The present approach will open up new opportunities for quantitative investigation of the pathophysiology of various neurodegenerative diseases.

Total-shear grating based optical diffraction tomography

2021 ◽  
Author(s):  
Piotr Zdańkowski ◽  
Julianna Winnik ◽  
Paweł Gocłowski ◽  
Maciej Trusiak
Keyword(s):  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography ◽  
Total Shear

scholarly journals Mitotic Chromosomes in Live Cells Characterized Using High-Speed and Label-Free Optical Diffraction Tomography

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1368 ◽  
Author(s):  
Kim ◽  
Lee ◽  
Fujii ◽  
Lee ◽  
Lee ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Optical Diffraction ◽  
Live Cells ◽  
Confocal Laser ◽  
Diffraction Tomography ◽  
Label Free ◽  
Mitotic Chromosomes ◽  
Optical Diffraction Tomography

The cell nucleus is a three-dimensional, dynamic organelle organized into subnuclear compartments such as chromatin and nucleoli. The structure and function of these compartments are maintained by diffusion and interactions between related factors as well as by dynamic and structural changes. Recent studies using fluorescent microscopic techniques suggest that protein factors can access and are freely mobile in heterochromatin and in mitotic chromosomes, despite their densely packed structure. However, the physicochemical properties of the chromosome during cell division are not fully understood. In the present study, characteristic properties such as the refractive index (RI), volume of the mitotic chromosomes, and diffusion coefficient (D) of fluorescent probes inside the chromosome were quantified using an approach combining label-free optical diffraction tomography with complementary confocal laser-scanning microscopy and fluorescence correlation spectroscopy. Variations in these parameters correlated with osmotic conditions, suggesting that changes in RI are consistent with those of the diffusion coefficient for mitotic chromosomes and cytosol. Serial RI tomography images of chromosomes in live cells during mitosis were compared with three-dimensional confocal micrographs to demonstrate that compaction and decompaction of chromosomes induced by osmotic change were characterized by linked changes in chromosome RI, volume, and the mobilities of fluorescent proteins.

Enhancement of optical resolution in three-dimensional refractive-index tomograms of biological samples by employing micromirror-embedded coverslips

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3484-3491 ◽  
Author(s):  
Seungwoo Shin ◽  
Jihye Kim ◽  
Je-Ryung Lee ◽  
Eun-chae Jeon ◽  
Tae-Jin Je ◽  
...  
Keyword(s):  
Refractive Index ◽  
Biological Samples ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

Resolution-enhanced optical diffraction tomography using a micromirror-embedded coverslips.

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