Prospects and challenges of quantitative phase imaging in tumor cell biology

2016 ◽  
Author(s):  
Björn Kemper ◽  
Martin Götte ◽  
Burkhard Greve ◽  
Steffi Ketelhut
Keyword(s):  
Tumor Cell ◽  
Cell Biology ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Tumor Cell Biology

scholarly journals Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Mikhail E. Kandel ◽  
Yuchen R. He ◽  
Young Jae Lee ◽  
Taylor Hsuan-Yu Chen ◽  
Kathryn Michele Sullivan ◽  
...  
Keyword(s):  
Cell Biology ◽  
Imaging System ◽  
High Specificity ◽  
Dry Mass ◽  
Live Cells ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Cell Nuclei ◽  
Quantitative Phase

AbstractDue to its specificity, fluorescence microscopy has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit fluorescence microscopy’s utility. Recently, it has been shown that artificial intelligence (AI) can transform one form of contrast into another. We present phase imaging with computational specificity (PICS), a combination of quantitative phase imaging and AI, which provides information about unlabeled live cells with high specificity. Our imaging system allows for automatic training, while inference is built into the acquisition software and runs in real-time. Applying the computed fluorescence maps back to the quantitative phase imaging (QPI) data, we measured the growth of both nuclei and cytoplasm independently, over many days, without loss of viability. Using a QPI method that suppresses multiple scattering, we measured the dry mass content of individual cell nuclei within spheroids. In its current implementation, PICS offers a versatile quantitative technique for continuous simultaneous monitoring of individual cellular components in biological applications where long-term label-free imaging is desirable.

scholarly journals Simultaneous measurement of neurite and neural body mass accumulation via quantitative phase imaging

The Analyst ◽  
2021 ◽  
Author(s):  
Soorya Pradeep ◽  
Tasmia Tasnim ◽  
Huanan Zhang ◽  
Thomas A. Zangle
Keyword(s):  
Body Mass ◽  
Biomass Production ◽  
Simultaneous Measurement ◽  
Neural Differentiation ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Mass Accumulation

Quantitative phase imaging (QPI) used to quantify the mass of soma (cell bodies) and neurites as well as the rates of biomass production due to neurite maturation and formation during neural differentiation.

scholarly journals High-speed line-field confocal holographic microscope for quantitative phase imaging

2016 ◽  
Vol 24 (9) ◽  
pp. 9251 ◽  
Author(s):  
Changgeng Liu ◽  
Sebastian Knitter ◽  
Zhilong Cong ◽  
Ikbal Sencan ◽  
Hui Cao ◽  
...  
Keyword(s):  
High Speed ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Line Field ◽  
Quantitative Phase

Analysis of edge effects in attenuating phase-shift masks using quantitative phase imaging

2013 ◽  
Author(s):  
Aamod Shanker ◽  
Martin Sczyrba ◽  
Brid Connolly ◽  
Andy Neureuther ◽  
Laura Waller
Keyword(s):  
Phase Shift ◽  
Edge Effects ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase
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