Label free imaging system for measuring blood flow speeds using a single multi-mode optical fiber (Conference Presentation)

2016 ◽  
Author(s):  
Iliya Sigal ◽  
Antonio M. Caravaca Aguirre ◽  
Raanan Gad ◽  
Rafael Piestun ◽  
Ofer Levi
Keyword(s):  
Blood Flow ◽  
Optical Fiber ◽  
Imaging System ◽  
Label Free ◽  
Flow Speeds ◽  
Multi Mode

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Label-free optical biosensor based on a dual-core microstructured polymer optical fiber

Optik ◽  
2015 ◽  
Vol 126 (21) ◽  
pp. 2930-2933 ◽  
Author(s):  
Nai-Fei Ren ◽  
Bing Sun ◽  
Ming-Yang Chen
Keyword(s):  
Optical Fiber ◽  
Optical Biosensor ◽  
Label Free ◽  
Polymer Optical Fiber ◽  
Dual Core

scholarly journals Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 813 ◽  
Author(s):  
Marta Janczuk-Richter ◽  
Beata Gromadzka ◽  
Łukasz Richter ◽  
Mirosława Panasiuk ◽  
Karolina Zimmer ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Medical Diagnostics ◽  
Label Free ◽  
Fiber Gratings ◽  
Fast Detection ◽  
Long Period ◽  
Highly Sensitive ◽  
Protein Receptors ◽  
Long Period Fiber Gratings ◽  
Vaccine Testing

Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user’s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.

scholarly journals Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System

Biosensors ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 471
Author(s):  
Hoibin Jeong ◽  
Song-Rae Kim ◽  
Yujung Kang ◽  
Huisu Kim ◽  
Seo-Young Kim ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Real Time ◽  
Tumor Angiogenesis ◽  
Imaging System ◽  
Angiogenesis Inhibitors ◽  
Laboratory Unit ◽  
Vessel Volume ◽  
Tumor Blood Vessels

Tumor angiogenesis is enhanced in all types of tumors to supply oxygen and nutrients for their growth and metastasis. With the development of anti-angiogenic drugs, the importance of technology that closely monitors tumor angiogenesis has also been emerging. However, to date, the technology for observing blood vessels requires specialized skills with expensive equipment, thereby limiting its applicability only to the laboratory setting. Here, we used a preclinical optical imaging system for small animals and, for the first time, observed, in real time, the entire process of blood vessel development in tumor-bearing mice injected with indocyanine green. Time-lapse sequential imaging revealed blood vessel volume and blood flow dynamics on a microscopic scale. Upon analyzing fluorescence dynamics at each stage of tumor progression, vessel volume and blood flow were found to increase as the tumor developed. Conversely, these vascular parameters decreased when the mice were treated with angiogenesis inhibitors, which suggests that the effects of drugs targeting angiogenesis can be rapidly and easily screened. The results of this study may help evaluate the efficacy of angiogenesis-targeting drugs by facilitating the observation of tumor blood vessels easily in a laboratory unit without large and complex equipment.

Calibration for single multi-mode fiber digital scanning microscopy imaging system

2015 ◽  
Author(s):  
Zhe Yin ◽  
Guodong Liu ◽  
Bingguo Liu ◽  
Yu Gan ◽  
Zhitao Zhuang ◽  
...  
Keyword(s):  
Imaging System ◽  
Scanning Microscopy ◽  
Microscopy Imaging ◽  
Digital Scanning ◽  
Multi Mode

scholarly journals Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules

Sensors ◽  
2011 ◽  
Vol 11 (4) ◽  
pp. 3780-3790 ◽  
Author(s):  
Ye Tian ◽  
Wenhui Wang ◽  
Nan Wu ◽  
Xiaotian Zou ◽  
Xingwei Wang
Keyword(s):  
Optical Fiber ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Label Free ◽  
Label Free Detection ◽  
Tapered Optical Fiber ◽  
Detection Of Biomolecules
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