Dead-time correction for high-throughput fluorescence lifetime imaging microscopy (Conference Presentation)

2016 ◽  
Author(s):  
Joerg Enderlein ◽  
Daja Ruhlandt ◽  
Anna Chithik ◽  
René Ebrecht ◽  
Fred S. Wouters ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Lifetime ◽  
Dead Time ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Dead Time Correction ◽  
Lifetime Imaging ◽  
Time Correction

scholarly journals Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

2016 ◽  
Vol 24 (9) ◽  
pp. 9429 ◽  
Author(s):  
Sebastian Isbaner ◽  
Narain Karedla ◽  
Daja Ruhlandt ◽  
Simon Christoph Stein ◽  
Anna Chizhik ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Dead Time ◽  
Fluorescence Lifetime Imaging ◽  
Dead Time Correction ◽  
Lifetime Measurements ◽  
Lifetime Imaging ◽  
Time Correction

scholarly journals A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues

2021 ◽  
Vol 9 ◽  
Author(s):  
Aric Bitton ◽  
Jesus Sambrano ◽  
Samantha Valentino ◽  
Jessica P. Houston
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Intrinsic Properties ◽  
Time Resolved ◽  
Lifetime Imaging ◽  
The Past ◽  
Biological Studies

Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization). Each has its own set of unique advantages, limitations, and challenges in detection when it comes to use in biological studies. This review will focus on the property of fluorescence lifetime, providing a brief background on instrumentation and theory, and examine the recent advancements and applications of measuring lifetime in the fields of high-throughput fluorescence lifetime imaging microscopy (HT-FLIM) and time-resolved flow cytometry (TRFC). In addition, the crossover of these two methods and their outlooks will be discussed.

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