In vivo imaging of cerebral hemodynamics and regional oxygen saturation in rats with a digital red-green-blue camera

2016 ◽  
Author(s):  
Izumi Nishidate ◽  
Yoshika Harasaki ◽  
Satoko Kawauchi ◽  
Shunichi Sato ◽  
Manabu Sato ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
In Vivo Imaging ◽  
Cerebral Hemodynamics ◽  
Regional Oxygen Saturation

In vivo imaging of tissue scattering parameter and cerebral hemodynamics in rat brain with a digital red-green-blue camera

2017 ◽  
Author(s):  
Izumi Nishidate ◽  
Afrina Mustari ◽  
Satoko Kawauchi ◽  
Shunichi Sato ◽  
Manabu Sato ◽  
...  
Keyword(s):  
Rat Brain ◽  
In Vivo Imaging ◽  
Cerebral Hemodynamics ◽  
Scattering Parameter ◽  
Tissue Scattering

In vivo imaging of spontaneous low-frequency oscillations in cerebral hemodynamics with a digital red-green-blue camera

2017 ◽  
Author(s):  
Afrina Mustari ◽  
Naoki Nakamura ◽  
Izumi Nishidate ◽  
Satoko Kawauchi ◽  
Shunichi Sato ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Low Frequency ◽  
Cerebral Hemodynamics ◽  
Low Frequency Oscillations

scholarly journals In Vivo Imaging of Cerebral Hemodynamics Using High-Frequency Micro-Ultrasound

2010 ◽  
Vol 2010 (9) ◽  
pp. pdb.prot5495-pdb.prot5495 ◽  
Author(s):  
J. Sun ◽  
L. Lindvere ◽  
M. E. van Raaij ◽  
A. Dorr ◽  
B. Stefanovic ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
High Frequency ◽  
Cerebral Hemodynamics

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo imaging reveals prostate pathology in the PTEN knockout murine model of prostate cancer

2016 ◽  
Author(s):  
Alysha Bhatti ◽  
Almeida Gilberto Serrano de ◽  
Serena Tommasini Ghelfi ◽  
Alwyn Dart ◽  
Anabel Varela-Carver ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Murine Model ◽  
In Vivo Imaging
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