Endoscope-based beveled and volume fiber-optic Raman probes for in vivo diagnosis of gastric dysplasia: a comparative study

2016 ◽  
Author(s):  
Jianfeng Wang ◽  
Kan Lin ◽  
Wei Zheng ◽  
Khek Yu Ho ◽  
Ming Teh ◽  
...  
Keyword(s):  
Comparative Study ◽  
Fiber Optic ◽  
Gastric Dysplasia ◽  
In Vivo Diagnosis

scholarly journals Real-time In vivo Diagnosis of Nasopharyngeal Carcinoma Using Rapid Fiber-Optic Raman Spectroscopy

Theranostics ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 3517-3526 ◽  
Author(s):  
Kan Lin ◽  
Wei Zheng ◽  
Chwee Ming Lim ◽  
Zhiwei Huang
Keyword(s):  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Real Time ◽  
Fiber Optic ◽  
In Vivo Diagnosis

Simultaneous fingerprint and high-wavenumber fiber-optic Raman endoscopy for in vivo diagnosis of laryngeal cancer

2016 ◽  
Author(s):  
Kan Lin ◽  
Wei Zheng ◽  
Jianfeng Wang ◽  
Chwee Ming Lim ◽  
Zhiwei Huang
Keyword(s):  
Laryngeal Cancer ◽  
Fiber Optic ◽  
In Vivo Diagnosis

scholarly journals Modelling, Design and Validation of Spatially Resolved Reflectance Based Fiber Optic Probe for Epithelial Precancer Diagnostics

2020 ◽  
Vol 10 (24) ◽  
pp. 8836
Author(s):  
Pankaj Singh ◽  
Prabodh Pandey ◽  
Shivam Shukla ◽  
Naren Naik ◽  
Asima Pradhan
Keyword(s):  
Fiber Optic ◽  
Numerical Study ◽  
Fiber Optic Probe ◽  
Scattering Coefficients ◽  
Spatially Resolved ◽  
Fiber Optic Probes ◽  
Diagnosis Of Cancer ◽  
Optic Probe ◽  
In Vivo Diagnosis

Fiber-optic probes are imperative for in-vivo diagnosis of cancer. Depending on the access to a diseased organ and the mutations one aims to sense, the probe designs vary. We carry out a detailed numerical study of the efficacy of the common probe geometries for epithelial cancer characterization based on spatially resolved reflectance data. As per the outcomes of this comparative study, a probe has been manufactured and using Monte Carlo look up table based inversion scheme, the absorption and scattering coefficients of the epithelium mimicking top layer have been recovered from noisy synthetic as well as experimental data.

scholarly journals Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman spectroscopy

2016 ◽  
Vol 7 (9) ◽  
pp. 3705 ◽  
Author(s):  
Kan Lin ◽  
Wei Zheng ◽  
Chwee Ming Lim ◽  
Zhiwei Huang
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Laryngeal Carcinoma ◽  
Fiber Optic ◽  
In Vivo Diagnosis

Fiber Optic Microneedles for Transdermal Light Delivery: Ex Vivo Porcine Skin Penetration Experiments

2010 ◽  
Vol 132 (9) ◽  
Author(s):  
Mehmet A. Kosoglu ◽  
Robert L. Hood ◽  
Ye Chen ◽  
Yong Xu ◽  
Marissa Nichole Rylander ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Fiber Optic ◽  
Ex Vivo ◽  
Skin Penetration ◽  
Average Diameter ◽  
Drawing Process ◽  
Pig Skin ◽  
In Vivo Diagnosis ◽  
Skin Samples

Shallow light penetration in tissue has been a technical barrier to the development of light-based methods for in vivo diagnosis and treatment of epithelial carcinomas. This problem can potentially be solved by utilizing minimally invasive probes to deliver light directly to target areas. To develop this solution, fiber optic microneedles capable of delivering light for either imaging or therapy were manufactured by tapering step-index silica-based optical fibers employing a melt-drawing process. Some of the microneedles were manufactured to have sharper tips by changing the heat source during the melt-drawing process. All of the microneedles were individually inserted into ex vivo pig skin samples to demonstrate the feasibility of their application in human tissues. The force on each microneedle was measured during insertion in order to determine the effects of sharper tips on the peak force and the steadiness of the increase in force. Skin penetration experiments showed that sharp fiber optic microneedles that are 3 mm long penetrate through 2 mm of ex vivo pig skin specimens. These sharp microneedles had a minimum average diameter of 73 μm and a maximum tip diameter of 8 μm. Flat microneedles, which had larger tip diameters, required a minimum average diameter of 125 μm in order to penetrate through pig skin samples. Force versus displacement plots showed that a sharp tip on a fiber optic microneedle decreased the skin’s resistance during insertion. Also, the force acting on a sharp microneedle increased more steadily compared with a microneedle with a flat tip. However, many of the sharp microneedles sustained damage during skin penetration. Two designs that did not accrue damage were identified and will provide a basis of more robust microneedles. Developing resilient microneedles with smaller diameters will lead to transformative, novel modes of transdermal imaging and treatment that are less invasive and less painful for the patient.

Fiber-optic Raman spectroscopy for in vivo diagnosis of gastric dysplasia

2016 ◽  
Vol 187 ◽  
pp. 377-392 ◽  
Author(s):  
Jianfeng Wang ◽  
Kan Lin ◽  
Wei Zheng ◽  
Khek Yu Ho ◽  
Ming Teh ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Raman Spectra ◽  
Fiber Optic ◽  
Endoscopic Examination ◽  
Great Promise ◽  
Gastric Tissue ◽  
Spectroscopy Technique ◽  
Gastric Dysplasia

This study aims to assess the clinical utility of a rapid fiber-optic Raman spectroscopy technique developed for enhancingin vivodiagnosis of gastric precancer during endoscopic examination. We have developed a real-time fiber-optic Raman spectroscopy system capable of simultaneously acquiring both fingerprint (FP) (i.e., 800–1800 cm−1) and high-wavenumber (HW) (i.e., 2800–3600 cm−1) Raman spectra from gastric tissuein vivoat endoscopy. A total of 5792 high-qualityin vivoFP/HW Raman spectra (normal (n= 5160); dysplasia (n= 155), and adenocarcinoma (n= 477)) were acquired in real-time from 441 tissue sites (normal (n= 396); dysplasia (n= 11), and adenocarcinoma (n= 34)) of 191 gastric patients (normal (n= 172); dysplasia (n= 6), and adenocarcinoma (n= 13)) undergoing routine endoscopic examinations. Partial least squares discriminant analysis (PLS-DA) together with leave-one-patient-out cross validation (LOPCV) were implemented to develop robust spectral diagnostic models. The FP/HW Raman spectra differ significantly between normal, dysplasia and adenocarcinoma of the stomach, which can be attributed to changes in proteins, lipids, nucleic acids, and the bound water content. PLS-DA and LOPCV show that the fiber-optic FP/HW Raman spectroscopy provides diagnostic sensitivities of 96.0%, 81.8% and 88.2%, and specificities of 86.7%, 95.3% and 95.6%, respectively, for the classification of normal, dysplastic and cancerous gastric tissue, superior to either the FP or HW Raman techniques alone. Further dichotomous PLS-DA analysis yields a sensitivity of 90.9% (10/11) and specificity of 95.9% (380/396) for the detection of gastric dysplasia using FP/HW Raman spectroscopy, substantiating its clinical advantages over white light reflectance endoscopy (sensitivity: 90.9% (10/11), and specificity: 51.0% (202/396)). This work demonstrates that the fiber-optic FP/HW Raman spectroscopy technique has great promise for enhancingin vivodiagnosis of gastric precancer during routine endoscopic examination.

A Comparative Study of the in vivo Distribution of 32P-Polyphosphates and 32P-Orthophosphate

1972 ◽  
Vol 11 (01) ◽  
pp. 70-78
Author(s):  
Esther Miller ◽  
Leopoldo Anghileri
Keyword(s):  
Radiation Dose ◽  
Comparative Study ◽  
Soft Tissues ◽  
Tumor Bearing ◽  
In Vivo Distribution ◽  
The Cross ◽  
Total Body

SummaryThe distribution of 32P-polyphosphates (lineal and cross-linked) and 32Porthophosphate in normal and tumor bearing animals has been studied. Differences between the cross-linked and the lineal form are related to a different degree of susceptibility to the hydrolysis by the phosphatases. In contrast to orthophosphate, the polyphosphates showed a lower accumulation in soft tissues which gives an advantageous reduction of the total body radiation dose.

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