Quantification of mammary organoid toxicant response and mammary tissue motility using OCT fluctuation spectroscopy (Conference Presentation)

2016 ◽  
Author(s):  
Xiao Yu ◽  
Richard L. Blackmon ◽  
Patricia Carabas-Hernendez ◽  
Ashley Fuller ◽  
Melissa A. Troester ◽  
...  
Keyword(s):  
Mammary Tissue

Formation and Structure of Casein Micelles in Lactating Mammary Tissue

1970 ◽  
Vol 28 ◽  
pp. 150-151
Author(s):  
Robert J. Carroll ◽  
Marvin P. Thompson ◽  
Harold M. Farrell
Keyword(s):  
Size Distribution ◽  
G Protein ◽  
Milk Proteins ◽  
Mammary Tissue ◽  
Colloidal System ◽  
Casein Micelles ◽  
The Stability

Milk is an unusually stable colloidal system; the stability of this system is due primarily to the formation of micelles by the major milk proteins, the caseins. Numerous models for the structure of casein micelles have been proposed; these models have been formulated on the basis of in vitro studies. Synthetic casein micelles (i.e., those formed by mixing the purified αsl- and k-caseins with Ca2+ in appropriate ratios) are dissimilar to those from freshly-drawn milks in (i) size distribution, (ii) ratio of Ca/P, and (iii) solvation (g. water/g. protein). Evidently, in vivo organization of the caseins into the micellar form occurs in-a manner which is not identical to the in vitro mode of formation.

Nutrient Uptake and Endocrine Regulation of Milk Synthesis by Mammary Tissue of Lactating Sows

1995 ◽  
Vol 73 (suppl_2) ◽  
pp. 36 ◽  
Author(s):  
Dean R Boyd ◽  
Ronald S Kensinger ◽  
Robert J Harrell ◽  
Dale E Bauman
Keyword(s):  
Nutrient Uptake ◽  
Mammary Tissue ◽  
Endocrine Regulation ◽  
Lactating Sows

scholarly journals Collagen molecular phenotypic switch between non-neoplastic and neoplastic canine mammary tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masahiko Terajima ◽  
Yuki Taga ◽  
Becky K. Brisson ◽  
Amy C. Durham ◽  
Kotaro Sato ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Mammary Carcinoma ◽  
Cancer Biology ◽  
Type I Collagen ◽  
Critical Role ◽  
Premature Death ◽  
Mammary Tissue ◽  
Type I ◽  
Cross Links

AbstractIn spite of major advances over the past several decades in diagnosis and treatment, breast cancer remains a global cause of morbidity and premature death for both human and veterinary patients. Due to multiple shared clinicopathological features, dogs provide an excellent model of human breast cancer, thus, a comparative oncology approach may advance our understanding of breast cancer biology and improve patient outcomes. Despite an increasing awareness of the critical role of fibrillar collagens in breast cancer biology, tumor-permissive collagen features are still ill-defined. Here, we characterize the molecular and morphological phenotypes of type I collagen in canine mammary gland tumors. Canine mammary carcinoma samples contained longer collagen fibers as well as a greater population of wider fibers compared to non-neoplastic and adenoma samples. Furthermore, the total number of collagen cross-links enriched in the stable hydroxylysine-aldehyde derived cross-links was significantly increased in neoplastic mammary gland samples compared to non-neoplastic mammary gland tissue. The mass spectrometric analyses of type I collagen revealed that in malignant mammary tumor samples, lysine residues, in particular those in the telopeptides, were markedly over-hydroxylated in comparison to non-neoplastic mammary tissue. The extent of glycosylation of hydroxylysine residues was comparable among the groups. Consistent with these data, expression levels of genes encoding lysyl hydroxylase 2 (LH2) and its molecular chaperone FK506-binding protein 65 were both significantly increased in neoplastic samples. These alterations likely lead to an increase in the LH2-mediated stable collagen cross-links in mammary carcinoma that may promote tumor cell metastasis in these patients.

Microemulsion for Prolonged Release of Fenretinide in the Mammary Tissue and Prevention of Breast Cancer Development

2021 ◽  
Author(s):  
Giovanna Cassone Salata ◽  
Isabella D. Malagó ◽  
Vanessa F. M. Carvalho Dartora ◽  
Ana Flávia Marçal Pessoa ◽  
Márcia Carvalho de Abreu Fantini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Development ◽  
Mammary Tissue ◽  
Prolonged Release ◽  
Breast Cancer Development ◽  
Prevention Of Breast Cancer

scholarly journals The Activation of Amino Acids in the Mammary Tissue of Rats

1961 ◽  
Vol 236 (7) ◽  
pp. 2015-2018
Author(s):  
Edsel T. Bucovaz ◽  
J. Wendell Davis
Keyword(s):  
Amino Acids ◽  
Mammary Tissue

scholarly journals Deciphering the temporal heterogeneity of cancer-associated fibroblast subpopulations in breast cancer

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Freja Albjerg Venning ◽  
Kamilla Westarp Zornhagen ◽  
Lena Wullkopf ◽  
Jonas Sjölund ◽  
Carmen Rodriguez-Cupello ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Ex Vivo ◽  
Smooth Muscle Actin ◽  
Mammary Tissue ◽  
Temporal Heterogeneity ◽  
Alpha Smooth Muscle Actin ◽  
Murine Tumours

Abstract Background Cancer-associated fibroblasts (CAFs) comprise a heterogeneous population of stromal cells within the tumour microenvironment. CAFs exhibit both tumour-promoting and tumour-suppressing functions, making them exciting targets for improving cancer treatments. Careful isolation, identification, and characterisation of CAF heterogeneity is thus necessary for ex vivo validation and future implementation of CAF-targeted strategies in cancer. Methods Murine 4T1 (metastatic) and 4T07 (poorly/non-metastatic) orthotopic triple negative breast cancer tumours were collected after 7, 14, or 21 days. The tumours were analysed via flow cytometry for the simultaneous expression of six CAF markers: alpha smooth muscle actin (αSMA), fibroblast activation protein alpha (FAPα), platelet derived growth factor receptor alpha and beta (PDGFRα and PDGFRβ), CD26/DPP4 and podoplanin (PDPN). All non-CAFs were excluded from the analysis using a lineage marker cocktail (CD24, CD31, CD45, CD49f, EpCAM, LYVE-1, and TER-119). In total 128 murine tumours and 12 healthy mammary fat pads were analysed. Results We have developed a multicolour flow cytometry strategy based on exclusion of non-CAFs and successfully employed this to explore the temporal heterogeneity of freshly isolated CAFs in the 4T1 and 4T07 mouse models of triple-negative breast cancer. Analysing 128 murine tumours, we identified 5–6 main CAF populations and numerous minor ones based on the analysis of αSMA, FAPα, PDGFRα, PDGFRβ, CD26, and PDPN. All markers showed temporal changes with a distinct switch from primarily PDGFRα+ fibroblasts in healthy mammary tissue to predominantly PDGFRβ+ CAFs in tumours. CD26+ CAFs emerged as a large novel subpopulation, only matched by FAPα+ CAFs in abundance. Conclusion We demonstrate that multiple subpopulations of CAFs co-exist in murine triple negative breast cancer, and that the abundance and dynamics for each marker differ depending on tumour type and time. Our results form the foundation needed to isolate and characterise specific CAF populations, and ultimately provide an opportunity to therapeutically target specific CAF subpopulations.

Mammary tissue damage during bovine mastitis: Causes and control1

2008 ◽  
Vol 86 (suppl_13) ◽  
pp. 57-65 ◽  
Author(s):  
X. Zhao ◽  
P. Lacasse
Keyword(s):  
Tissue Damage ◽  
Bovine Mastitis ◽  
Mammary Tissue

Establishing the mammary tissue microbiome

The Breast ◽  
2019 ◽  
Vol 44 ◽  
pp. S136
Author(s):  
N. Narayanen ◽  
G. Hogan ◽  
D. O'hanlon ◽  
M. Tangney
Keyword(s):  
Mammary Tissue

Ultrastructure and Frequency of Mastitis Caused by Ovine Progressive Pneumonia Virus Infection in Sheep

1986 ◽  
Vol 23 (2) ◽  
pp. 184-189 ◽  
Author(s):  
P. Deng ◽  
R. C. Cutlip ◽  
H. D. Lehmkuhl ◽  
K. A. Brogden
Keyword(s):  
Virus Infection ◽  
Mammary Glands ◽  
Infected Sheep ◽  
Mammary Tissue ◽  
Target Organs ◽  
Cellular Debris ◽  
Ovine Progressive Pneumonia

Twenty-five sheep, experimentally ( n = 15) or naturally ( n = 6) infected with ovine progressive pneumonia virus and noninfected controls ( n = 4), were evaluated for histological and ultrastructural lesions of mastitis. Histologically, nine of 15 experimentally infected sheep and all six naturally infected sheep had lympho-plasmacytic mastitis. Severity of the lesion increased with length of time after infection. Periductal lymphatic nodules were seen in five sheep experimentally infected for 2.8 years or longer and in five naturally infected sheep that wore 3.7 years old or older. Ultrastructurally, responses to ovine progressive pneumonia virus were diffuse lympho-plasmacytic infiltrates in glandular interstitium, lymphocytic and occasional plasmacytic infiltrates in ductal walls and lumens, lymphoblasts surrounded by small lymphocytes in glandular interstitium, and degeneration of epithelium releasing cells and cellular debris into the lumen. Based on the prevalence of lesions, the mammary tissue was more susceptible to ovine progressive pneumonia virus than other target organs: lung, brain, and synovium. Lesions did not differ between breeds of sheep. Ovine progressive pneumonia virus was not seen in the mammary tissue but was isolated from 15 of 17 mammary glands.

NEURAL FACTORS IN THE MAINTENANCE OF LACTATION IN THE RAT

1967 ◽  
Vol 38 (1) ◽  
pp. 51-59 ◽  
Author(s):  
J. A. EDWARDSON ◽  
J. T. EAYRS
Keyword(s):  
Litter Size ◽  
Milk Yield ◽  
Quantitative Relationship ◽  
Mammary Tissue ◽  
Single Pair ◽  
Endocrine Response ◽  
Peripheral Innervation ◽  
Lactational Performance ◽  
Neural Factors

SUMMARY The role of the peripheral innervation of mammary tissue in the maintenance of lactation has been investigated by the procedure of selective thelectomy combined with denervation of the posterior thoracic nipples. When suckling is restricted to a single pair of nipples bilateral transection of the three adjacent nerves supplying a nipple arrests lactation completely; partial denervation is associated with a reduced level of lactational performance which is directly related to the concentration of the residual innervation. Increase in litter size is associated with an overall increase in milk-yield up to a limit beyond which the addition of further young to the litter is without effect. It is inferred that there is a quantitative relationship between the neural stimulus of suckling and the endocrine response of the hypothalamopituitary system.

Sign in / Sign up

Export Citation Format

Share Document