Label-free tracking of single extracellular vesicles in a nano-fluidic optical fiber (Conference Presentation)

2016 ◽  
Author(s):  
Edwin van der Pol ◽  
Stefan Weidlich ◽  
Yoav Lahini ◽  
Frank A. W. Coumans ◽  
Auguste Sturk ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Extracellular Vesicles ◽  
Label Free

Label-free optical biosensor based on a dual-core microstructured polymer optical fiber

Optik ◽  
2015 ◽  
Vol 126 (21) ◽  
pp. 2930-2933 ◽  
Author(s):  
Nai-Fei Ren ◽  
Bing Sun ◽  
Ming-Yang Chen
Keyword(s):  
Optical Fiber ◽  
Optical Biosensor ◽  
Label Free ◽  
Polymer Optical Fiber ◽  
Dual Core

scholarly journals Temporal Quantitative Proteomics Analysis of Neuroblastoma Cells Treated with Bovine Milk-Derived Extracellular Vesicles Highlights the Anti-Proliferative Properties of Milk-Derived Extracellular Vesicles

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 750
Author(s):  
Pamali Fonseka ◽  
Taeyoung Kang ◽  
Sing Chee ◽  
Sai V. Chitti ◽  
Rahul Sanwlani ◽  
...  
Keyword(s):  
High Risk ◽  
Extracellular Vesicles ◽  
Quantitative Proteomics ◽  
Cell Metabolism ◽  
Bovine Milk ◽  
Neuroblastoma Cells ◽  
Treatment Strategies ◽  
Treatment Resistance ◽  
Label Free ◽  
The Impact

Neuroblastoma (NBL) is a pediatric cancer that accounts for 15% of childhood cancer mortality. Amplification of the oncogene N-Myc occurs in 20% of NBL patients and is considered high risk as it correlates with aggressiveness, treatment resistance and poor prognosis. Even though the treatment strategies have improved in the recent years, the survival rate of high-risk NBL patients remain poor. Hence, it is crucial to explore new therapeutic avenues to sensitise NBL. Recently, bovine milk-derived extracellular vesicles (MEVs) have been proposed to contain anti-cancer properties. However, the impact of MEVs on NBL cells is not understood. In this study, we characterised MEVs using Western blotting, NTA and TEM. Importantly, treatment of NBL cells with MEVs decreased the proliferation and increased the sensitivity of NBL cells to doxorubicin. Temporal label-free quantitative proteomics of NBL cells highlighted the depletion of proteins involved in cell metabolism, cell growth and Wnt signalling upon treatment with MEVs. Furthermore, proteins implicated in cellular senescence and apoptosis were enriched in NBL cells treated with MEVs. For the first time, this study highlights the temporal proteomic profile that occurs in cancer cells upon MEVs treatment.

scholarly journals Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 813 ◽  
Author(s):  
Marta Janczuk-Richter ◽  
Beata Gromadzka ◽  
Łukasz Richter ◽  
Mirosława Panasiuk ◽  
Karolina Zimmer ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Medical Diagnostics ◽  
Label Free ◽  
Fiber Gratings ◽  
Fast Detection ◽  
Long Period ◽  
Highly Sensitive ◽  
Protein Receptors ◽  
Long Period Fiber Gratings ◽  
Vaccine Testing

Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user’s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.

scholarly journals Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules

Sensors ◽  
2011 ◽  
Vol 11 (4) ◽  
pp. 3780-3790 ◽  
Author(s):  
Ye Tian ◽  
Wenhui Wang ◽  
Nan Wu ◽  
Xiaotian Zou ◽  
Xingwei Wang
Keyword(s):  
Optical Fiber ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Label Free ◽  
Label Free Detection ◽  
Tapered Optical Fiber ◽  
Detection Of Biomolecules

scholarly journals Label-Free Prostate Cancer Detection by Characterization of Extracellular Vesicles Using Raman Spectroscopy

2018 ◽  
Vol 90 (19) ◽  
pp. 11290-11296 ◽  
Author(s):  
Wooje Lee ◽  
Afroditi Nanou ◽  
Linda Rikkert ◽  
Frank A. W. Coumans ◽  
Cees Otto ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Raman Spectroscopy ◽  
Extracellular Vesicles ◽  
Cancer Detection ◽  
Label Free ◽  
Prostate Cancer Detection

The cholesterol metabolite 27-hydroxycholesterol increases the secretion of extracellular vesicles which promote breast cancer progression

Endocrinology ◽  
2021 ◽  
Author(s):  
Amy E Baek ◽  
Natalia Krawczynska ◽  
Anasuya Das Gupta ◽  
Svyatoslav Victorovich Dvoretskiy ◽  
Sixian You ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Cancer Progression ◽  
Myeloid Cells ◽  
Breast Cancer Progression ◽  
Label Free ◽  
Tumor Growth And Metastasis ◽  
Promote Breast Cancer

Abstract Cholesterol has been implicated in the clinical progression of breast cancer, a disease that continues to be the most commonly diagnosed cancer in women. Previous work has identified the cholesterol metabolite, 27-hydroxycholesterol (27HC), as a major mediator of the effects of cholesterol on breast tumor growth and progression. 27HC can act as an estrogen receptor (ER) modulator to promote the growth of ERα+ tumors, and a liver x receptor (LXR) ligand in myeloid immune cells to establish an immune-suppressive program. In fact, the metastatic properties of 27HC require the presence of myeloid cells, with neutrophils (PMNs) being essential for the increase in lung metastasis in murine models. In an effort to further elucidate the mechanisms by which 27HC alters breast cancer progression, we made the striking finding that 27HC promoted the secretion of extracellular vesicles (EVs), a diverse assortment of membrane bound particles that include exosomes. The resulting EVs had a size distribution that was skewed slightly larger, compared to EVs generated by treating cells with vehicle. The increase in EV secretion and size was consistent across three different subtypes: primary murine PMNs, RAW264.7 monocytic cells and 4T1 murine mammary cancer cells. Label-free analysis of 27HC-EVs indicated that they had a different metabolite composition to those from vehicle-treated cells. Importantly, 27HC-EVs from primary PMNs promoted tumor growth and metastasis in two different syngeneic models, demonstrating the potential role of 27HC induced EVs in the progression of breast cancer. EVs from PMNs were taken up by cancer cells, macrophages and PMNs, but not T cells. Since EVs did not alter proliferation of cancer cells, it is likely that their pro-tumor effects are mediated through interactions with myeloid cells. Interestingly, RNA-seq analysis of tumors from 27HC-EV treated mice do not display significantly altered transcriptomes, suggesting that the effects of 27HC-EVs occur early on in tumor establishment and growth. Future work will be required to elucidate the mechanisms by which 27HC increases EV secretion, and how these EVs promote breast cancer progression. Collectively however, our data indicate that EV secretion and content can be regulated by a cholesterol metabolite, which may have detrimental effects in terms of disease progression, important findings given the prevalence of both breast cancer and hypercholesterolemia.

scholarly journals Rapid and label-free isolation of small extracellular vesicles from biofluids utilizing a novel insulator based dielectrophoretic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (21) ◽  
pp. 3726-3734 ◽  
Author(s):  
Leilei Shi ◽  
Damaris Kuhnell ◽  
Vishnupriya J. Borra ◽  
Scott M. Langevin ◽  
Takahisa Nakamura ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Low Voltage ◽  
High Yield ◽  
Label Free ◽  
Initial Sample

Rapid and low voltage iDEP device for purification of exosomes from biofluids with high yield and small initial sample volumes.

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