scholarly journals Binding of the immunomodulatory drug Bz-423 to mitochondrial FoF1-ATP synthase in living cells by FRET acceptor photobleaching

2016 ◽  
Author(s):  
Ilka Starke ◽  
Kathryn M. Johnson ◽  
Jan Petersen ◽  
Peter Gräber ◽  
Anthony W. Opipari ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Living Cells ◽  
Acceptor Photobleaching ◽  
Immunomodulatory Drug ◽  
Fof1 Atp Synthase

scholarly journals The Regulatory C-Terminal Domain of Subunit   of FoF1 ATP Synthase Is Dispensable for Growth and Survival of Escherichia coli

2011 ◽  
Vol 193 (8) ◽  
pp. 2046-2052 ◽  
Author(s):  
N. Taniguchi ◽  
T. Suzuki ◽  
M. Berney ◽  
M. Yoshida ◽  
G. M. Cook
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Growth And Survival ◽  
Terminal Domain ◽  
Fof1 Atp Synthase

scholarly journals Single-molecule Imaging of ATP-driven Stepping Rotation of FoF1-ATP Synthase Reconstituted into Supported Membrane

2009 ◽  
Vol 96 (3) ◽  
pp. 29a
Author(s):  
Ryota Iino ◽  
Khek-Chian Tham ◽  
Kazuhito V. Tabata ◽  
Hiroshi Ueno ◽  
Hiroyuki Noji
Keyword(s):  
Atp Synthase ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Supported Membrane ◽  
Fof1 Atp Synthase

Electrophysiological properties of channel formed by bovine FOF1 ATP synthase in planar lipid bilayer

2018 ◽  
Vol 1859 ◽  
pp. e84
Author(s):  
Andrea Urbani ◽  
Valentina Giorgio ◽  
Chimari Jiko ◽  
Janna F.M. Bogers ◽  
Duncan G.G. McMillan ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Atp Synthase ◽  
Planar Lipid Bilayer ◽  
Electrophysiological Properties ◽  
Fof1 Atp Synthase

scholarly journals Cyclophilin D isomerase activity controls FoF1-ATP synthase oligomerization and mitochondrial ultrastructure

2018 ◽  
Vol 1859 ◽  
pp. e16
Author(s):  
Rubén Quintana-Cabrera ◽  
Mauro Corrado ◽  
Michella Carraro ◽  
Valentina Giorgio ◽  
María Eugenia Soriano ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Cyclophilin D ◽  
Mitochondrial Ultrastructure ◽  
Isomerase Activity ◽  
Fof1 Atp Synthase

scholarly journals Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

2009 ◽  
Vol 106 (37) ◽  
pp. 15651-15656 ◽  
Author(s):  
Hiromi Imamura ◽  
Kim P. Huynh Nhat ◽  
Hiroko Togawa ◽  
Kenta Saito ◽  
Ryota Iino ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Atp Synthase ◽  
Hela Cells ◽  
Dissociation Constants ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Atp Levels ◽  
Fluorescence Resonance

Adenosine 5′-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside individual living cells. To visualize ATP levels, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP that were composed of the ε subunit of the bacterial FoF1-ATP synthase sandwiched by the cyan- and yellow-fluorescent proteins. The indicators, named ATeams, had apparent dissociation constants for ATP ranging from 7.4 μM to 3.3 mM. By targeting ATeams to different subcellular compartments, we unexpectedly found that ATP levels in the mitochondrial matrix of HeLa cells are significantly lower than those of cytoplasm and nucleus. We also succeeded in measuring changes in the ATP level inside single HeLa cells after treatment with inhibitors of glycolysis and/or oxidative phosphorylation, revealing that glycolysis is the major ATP-generating pathway of the cells grown in glucose-rich medium. This was also confirmed by an experiment using oligomycin A, an inhibitor of FoF1-ATP synthase. In addition, it was demonstrated that HeLa cells change ATP-generating pathway in response to changes of nutrition in the environment.

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