A study on the characteristics of the Analog Mean Delay (AMD) method for high-speed Fluorescence Lifetime Imaging Microscopy (FLIM)

2016 ◽  
Author(s):  
Byungyeon Kim ◽  
Byungjun Park ◽  
Seungrag Lee ◽  
Youngjae Won
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Mean Delay

scholarly journals High-speed confocal fluorescence lifetime imaging microscopy (FLIM) with the analog mean delay (AMD) method

2011 ◽  
Vol 19 (4) ◽  
pp. 3396 ◽  
Author(s):  
Youngjae Won ◽  
Sucbei Moon ◽  
Wenzhong Yang ◽  
Donguk Kim ◽  
Won-Taek Han ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Mean Delay ◽  
Confocal Fluorescence

scholarly journals High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy

Optica ◽  
2018 ◽  
Vol 5 (10) ◽  
pp. 1290 ◽  
Author(s):  
Andrew J. Bower ◽  
Joanne Li ◽  
Eric J. Chaney ◽  
Marina Marjanovic ◽  
Darold R. Spillman ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
High Speed Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy

2019 ◽  
Vol 10 (12) ◽  
pp. 6408 ◽  
Author(s):  
Andrew J. Bower ◽  
Janet E. Sorrells ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells

2020 ◽  
Author(s):  
Yayao Ma ◽  
Youngjae Lee ◽  
Catherine Best-Popescu ◽  
Liang Gao
Keyword(s):  
Compressed Sensing ◽  
Fluorescence Lifetime ◽  
High Speed ◽  
Time Lapse ◽  
Live Cells ◽  
Large Field ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Specific Domain ◽  
Lifetime Imaging

AbstractWe present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale.

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