A single-shot 2D/3D simultaneous imaging microscope based on light field microscopy

Author(s):  
Jonghyun Kim ◽  
Youngmin Kim ◽  
Youngmo Jeong ◽  
Byoungho Lee
Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1453
Author(s):  
Hyun Myung Kim ◽  
Min Seok Kim ◽  
Sehui Chang ◽  
Jiseong Jeong ◽  
Hae-Gon Jeon ◽  
...  

The light field camera provides a robust way to capture both spatial and angular information within a single shot. One of its important applications is in 3D depth sensing, which can extract depth information from the acquired scene. However, conventional light field cameras suffer from shallow depth of field (DoF). Here, a vari-focal light field camera (VF-LFC) with an extended DoF is newly proposed for mid-range 3D depth sensing applications. As a main lens of the system, a vari-focal lens with four different focal lengths is adopted to extend the DoF up to ~15 m. The focal length of the micro-lens array (MLA) is optimized by considering the DoF both in the image plane and in the object plane for each focal length. By dividing measurement regions with each focal length, depth estimation with high reliability is available within the entire DoF. The proposed VF-LFC is evaluated by the disparity data extracted from images with different distances. Moreover, the depth measurement in an outdoor environment demonstrates that our VF-LFC could be applied in various fields such as delivery robots, autonomous vehicles, and remote sensing drones.


Sensors ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 500 ◽  
Author(s):  
Luca Palmieri ◽  
Gabriele Scrofani ◽  
Nicolò Incardona ◽  
Genaro Saavedra ◽  
Manuel Martínez-Corral ◽  
...  

Light field technologies have seen a rise in recent years and microscopy is a field where such technology has had a deep impact. The possibility to provide spatial and angular information at the same time and in a single shot brings several advantages and allows for new applications. A common goal in these applications is the calculation of a depth map to reconstruct the three-dimensional geometry of the scene. Many approaches are applicable, but most of them cannot achieve high accuracy because of the nature of such images: biological samples are usually poor in features and do not exhibit sharp colors like natural scene. Due to such conditions, standard approaches result in noisy depth maps. In this work, a robust approach is proposed where accurate depth maps can be produced exploiting the information recorded in the light field, in particular, images produced with Fourier integral Microscope. The proposed approach can be divided into three main parts. Initially, it creates two cost volumes using different focal cues, namely correspondences and defocus. Secondly, it applies filtering methods that exploit multi-scale and super-pixels cost aggregation to reduce noise and enhance the accuracy. Finally, it merges the two cost volumes and extracts a depth map through multi-label optimization.


Author(s):  
Oliver Garbrecht ◽  
José Zapata ◽  
Reinhold Kneer

The shape of an installed solar concentrator (e.g. a heliostat) may differ from its original design due to manufacturing defects, structural/wind loads and thermal expansion. By measuring the shape of a solar concentrator, it is possible to account for the deviation in optical performance from its original design point. A method to measure concentrator shape needs to be fast, accurate, and not involve contact or interference with the reflective surface of the concentrator. State-of-the-art techniques include flux mapping, photogrammetry, and deflectometry using conventional cameras. This paper presents a study to characterise solar concentrator shapes using light-field imaging. Conventional cameras capture the light intensity of a point in a scene at a single point in the sensor, creating a two-dimensional image. A light field camera features multiple micro-lenses placed between the main lens and the sensor, providing many small images from slightly different angles in a single shot. This information is used to reconstruct the position of a light source in space. The advantage of this new technique to the ones mentioned above is that the light field camera is robust and self-contained, which allows easy-to-use application in heliostat fields. In this study, light-field camera measurements were performed with flat mirrors and a curved mirror under laboratory conditions. In order to resolve the surface of the mirror surfaces, several methods to impose contours of the mirror surface have been studied, including dirt, small water droplets, scattering of low-power laser light, and paper-marks. A wide range of camera-to-mirror distances between 43 cm and 5 m have been studied. Greater distances allow the capture of the entire surface, but decrease the precision of depth measurements. In order to obtain high precision measurements while being able to capture the entire surface, a compositing strategy has been developed, combining several light-field image measurements. The overall accuracy of the system was improved further by averaging measurements over several image frames. Subsequently, the reconstructed surface points have been fed to a ray-tracing algorithm realized in Matlab/Python. Results in this study are able to resolve the shape of small concentrators to sub-millimetre precision when taking pictures at a distance of 0.4 m.


2021 ◽  
Author(s):  
Luca Palmieri

Microlens-array based plenoptic cameras capture the light field in a single shot, enabling new potential applications but also introducing additional challenges. A plenoptic image consists of thousand of microlens images. Estimating the disparity for each microlens allows to render conventional images, changing the perspective and the focal settings, and to reconstruct the three-dimensional geometry of the scene. The work includes a blur-aware calibration method to model plenoptic cameras, an optimization method to accurately select the best microlenses combination for disparity estimation, an overview of the different types of plenoptic cameras, an analysis of the disparity estimation algorithms, and a robust depth estimation approach for light field microscopy. The research led to the creation of a full framework for plenoptic cameras, which contains the implementation of the algorithms discussed in the work and datasets of both real and synthetic images for comparison, benchmarking and future research.


2020 ◽  
Vol 6 (12) ◽  
pp. 138
Author(s):  
Nicola Viganò ◽  
Felix Lucka ◽  
Ombeline de La Rochefoucauld ◽  
Sophia Bethany Coban ◽  
Robert van Liere ◽  
...  

X-ray plenoptic cameras acquire multi-view X-ray transmission images in a single exposure (light-field). Their development is challenging: designs have appeared only recently, and they are still affected by important limitations. Concurrently, the lack of available real X-ray light-field data hinders dedicated algorithmic development. Here, we present a physical emulation setup for rapidly exploring the parameter space of both existing and conceptual camera designs. This will assist and accelerate the design of X-ray plenoptic imaging solutions, and provide a tool for generating unlimited real X-ray plenoptic data. We also demonstrate that X-ray light-fields allow for reconstructing sharp spatial structures in three-dimensions (3D) from single-shot data.


2020 ◽  
Vol 45 (12) ◽  
pp. 3256
Author(s):  
Zewei Cai ◽  
Giancarlo Pedrini ◽  
Wolfgang Osten ◽  
Xiaoli Liu ◽  
Xiang Peng

2021 ◽  
pp. 127130
Author(s):  
Tianlei Ning ◽  
Yanqiu Li ◽  
Guodong Zhou ◽  
Ke Liu ◽  
Jiazhi Wang

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3803 ◽  
Author(s):  
Jaewon Kim ◽  
Abhijeet Ghosh

We present a novel computational photography technique for single-shot separation of diffuse/specular reflectance, as well as novel angular domain separation of layered reflectance. We present two imaging solutions for this purpose: two-way polarized light-field (TPLF) imaging and four-way polarized light-field (FPLF) imaging. TPLF imaging consists of a polarized light-field camera, which simultaneously captures two orthogonal states of polarization. A single photograph of a subject acquired with the TPLF camera under polarized illumination then enables standard separation of diffuse (depolarizing) and polarization preserving specular reflectance using light-field sampling. We further demonstrate that the acquired data also enable novel angular separation of layered reflectance including separation of specular reflectance and single scattering in the polarization preserving component, as well as separation of shallow scattering from deep scattering in the depolarizing component. FPLF imaging further generalized the functionality of TPLF imaging under uncontrolled unpolarized or partially polarized illumination such as outdoors. We apply our approach for efficient acquisition of facial reflectance including diffuse and specular normal maps and novel separation of photometric normals into layered reflectance normals for layered facial renderings. We validate our proposed single-shot layered reflectance separation under various imaging conditions and demonstrate it to be comparable to an existing multi-shot technique that relies on structured lighting while achieving separation results under a variety of illumination conditions.


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