Plasmonically amplified fluorescence bioassay with microarray format

2015 ◽  
Author(s):  
S. Gogalic ◽  
S. Hageneder ◽  
C. Ctortecka ◽  
M. Bauch ◽  
I. Khan ◽  
...  
Keyword(s):  
Microarray Format

Exploring Nature's plasticity with a flexible probing tool, and finding new ways for its electronic distribution

2002 ◽  
Vol 30 (2) ◽  
pp. 78-82 ◽  
Author(s):  
M. Beier ◽  
M. Baum ◽  
H. Rebscher ◽  
R. Mauritz ◽  
A. Wixmerten ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Situ Synthesis ◽  
Light Modulation ◽  
Genomic Assays ◽  
Electronic Distribution ◽  
Spatial Light Modulation ◽  
Microarray Fabrication ◽  
Set Up ◽  
Microarray Format

Concepts and results are described for the use of a single, but extremely flexible, probing tool to address a wide variety of genomic questions. This is achieved by transforming genomic questions into a software file that is used as the design scheme for potentially any genomic assay in a microarray format. Microarray fabrication takes place in three-dimensional microchannel reaction carriers by in situ synthesis based on spatial light modulation. This set-up allows for maximum flexibility in design and realization of genomic assays. Flexibility is achieved at the molecular, genomic and assay levels. We have applied this technology to expression profiling and genotyping experiments.

scholarly journals Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

2019 ◽  
Vol 25 (2) ◽  
pp. 132-139
Author(s):  
Yaw-Jen Chang ◽  
Hong-Wei Yang ◽  
Len-Hao Yao ◽  
Wen-Tung Yang
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Intensity ◽  
Continuous Flow ◽  
Main Channel ◽  
Tumor Suppressor Protein ◽  
Small Droplet ◽  
Chip Surface ◽  
Tumor Suppressor Protein P53 ◽  
Extracellular Signal ◽  
Microarray Format

This paper presents a droplet-based immunoassay chip allowing each droplet to be positioned in a passive droplet-positioning cavern under continuous flow. In addition, the chip surface can immobilize any kind of histidine-tagged capture agents for performing simultaneous multiplex immunoassays. Distinct families of monodispersed droplets were generated since a diaphragm, which is a thin elastomeric flap film suspended from the top of the main channel, forms a double T junction for shearing the aqueous liquids by the carrier flow. These two types of monodispersed droplets traverse the main channel to the downstream detection area and enter the passive positioning caverns for further immunoassay. A layer of Ni–Co film was coated on the substrate by electrodeposition in order to immobilize the multiplex histidine-tagged capture molecules. In this study, the tumor suppressor protein p53 and the extracellular signal-related kinase 1 (ERK1) were used as the capture agents. Then, both histidine-tagged proteins p53 and ERK1 were immobilized by the Ni–Co layer in a microarray format for subsequent immunoassay and fluorescence detection. The experimental results show that the detected fluorescence intensity is proportioned to the concentration of the encapsulated content in a small droplet. This proposed droplet-based immunoassay chip can immobilize multiplex histidine-tagged proteins, irrelevant to the species of proteins, to carry out simultaneous immunoassays and allow the operation sequence to be conducted automatically through the manipulation of droplets.

Life on a microarray: assessing live cell functions in a microarray format

2012 ◽  
Vol 69 (16) ◽  
pp. 2717-2725 ◽  
Author(s):  
Krisztián Papp ◽  
Zoltán Szittner ◽  
József Prechl
Keyword(s):  
Live Cell ◽  
Cell Functions ◽  
Microarray Format

Differentiation of Cucumber mosaic virus isolates by hybridization to oligonucleotides in a microarray format

2005 ◽  
Vol 123 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Zhang Deyong ◽  
Peter Willingmann ◽  
Cornelia Heinze ◽  
Günter Adam ◽  
Monica Pfunder ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Isolates ◽  
Microarray Format
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