Three-dimensional microscopy through liquid-lens axial scanning

2015 ◽  
Author(s):  
Ana Doblas ◽  
E. Sánchez-Ortiga ◽  
G. Saavedra ◽  
J. Sola-Pikabea ◽  
M. Martínez-Corral ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Liquid Lens ◽  
Axial Scanning

scholarly journals Fast axial scanning for 2-photon microscopy using liquid lens technology

2017 ◽  
Author(s):  
Kayvan Forouhesh Tehrani ◽  
Min Kyoung Sun ◽  
Lohitash Karumbaiah ◽  
Luke J. Mortensen
Keyword(s):  
Liquid Lens ◽  
Axial Scanning

scholarly journals Three dimensional two-photon imaging of neuronal activity in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

2018 ◽  
Author(s):  
Baris N. Ozbay ◽  
Gregory L. Futia ◽  
Ming Ma ◽  
Victor M. Bright ◽  
Juliet T. Gopinath ◽  
...  
Keyword(s):  
Dynamic Control ◽  
Three Dimensional ◽  
Neuron Activity ◽  
Two Photon ◽  
Neuronal Imaging ◽  
Freely Moving ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Working Distance ◽  
Axial Scanning

AbstractWe present a miniature head mounted two-photon fiber-coupled microscope (2P-FCM) for neuronal imaging with active axial focusing enabled using a miniature electrowetting lens. Full three-dimensional two-photon imaging of GCaMP6s showing individual neuron activity in multiple focal planes was achieved in a freely-moving mouse. Two-color simultaneous imaging of GFP and tdTomato fluorescence is also demonstrated. Additionally, dynamic control of the axial scanning of the electrowetting lens allows tilting of the focal plane enabling cells in multiple focal planes to be imaged simultaneously. Two-photon imaging allows increased penetration depth in tissue yielding a working distance of 450 μm with an additional 180 μm of active axial focusing. The objective NA is 0.45 with a lateral resolution of 1.8 μm, an axial resolution of 10 μm, and a field-of-view of 240 μm diameter. The 2P-FCM has a weight of only ∼2.5 g and is capable of repeatable and stable head-attachment. The 2P-FCM with dynamic axial scanning provides a new capability to record from functionally distinct neuronal layers, opening new opportunities in neuroscience research.

Three-dimensional adaptive microscopy using embedded liquid lens

2009 ◽  
Vol 34 (2) ◽  
pp. 145 ◽  
Author(s):  
Supraja Murali ◽  
Kevin P. Thompson ◽  
Jannick P. Rolland
Keyword(s):  
Three Dimensional ◽  
Liquid Lens

Liquid lens-based optical sectioning tomography for three-dimensional flame temperature measurement

Fuel ◽  
2017 ◽  
Vol 196 ◽  
pp. 550-563 ◽  
Author(s):  
Chuanlong Xu ◽  
Wenchao Zhao ◽  
Jianghai Hu ◽  
Biao Zhang ◽  
Shimin Wang
Keyword(s):  
Temperature Measurement ◽  
Flame Temperature ◽  
Three Dimensional ◽  
Optical Sectioning ◽  
Liquid Lens

scholarly journals Large-scale calcium imaging with a head-mount axial scanning 3D fluorescence microscope

2021 ◽  
Author(s):  
Yuichiro Hayashi ◽  
Ko Kobayakawa ◽  
Reiko Kobayakawa
Keyword(s):  
Calcium Imaging ◽  
Large Scale ◽  
Low Cost ◽  
3D Structure ◽  
Three Dimensional ◽  
Brain Structures ◽  
Volumetric Imaging ◽  
Brain Functions ◽  
Local Circuits ◽  
Axial Scanning

AbstractMiniaturized fluorescence microscopes are becoming more important for deciphering the neural codes underlying various brain functions. Using gradient index (GRIN) lenses, these devices enable the recording of neuronal activity in deep brain structures. However, to minimize any damage to brain tissue and local circuits, the diameter of the GRIN lens should be 0.5–1 mm, which results in a small field of view. Considering the three-dimensional (3D) structure of neural circuits in the brain, volumetric imaging capability would increase the number of neurons imaged through the lenses. To observe 3D calcium dynamics, we developed a miniaturized microscope with an electrically tunable lens. Using this microscope, we performed 3D calcium imaging in behaving mice and were able to image approximately twice the number of cells as could be recorded using a 2D imaging technique. This simple low-cost 3D microscope will improve the efficiency of calcium imaging in behaving animals.

scholarly journals Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections

2019 ◽  
Author(s):  
Martin Pauli ◽  
Mila M. Paul ◽  
Sven Proppert ◽  
Marzieh Sharifi ◽  
Felix Repp ◽  
...  
Keyword(s):  
Single Molecule ◽  
Mossy Fiber ◽  
Three Dimensional ◽  
Brain Regions ◽  
Molecular Organization ◽  
Localization Microscopy ◽  
Stochastic Optical Reconstruction Microscopy ◽  
Optical Reconstruction ◽  
Axial Scanning ◽  
Single Molecule Localization Microscopy

ABSTRACTRevealing the molecular organization of anatomically precisely defined brain regions is necessary for the refined understanding of synaptic plasticity. Although, three-dimensional (3D) single-molecule localization microscopy can provide the required molecular resolution, single-molecule imaging more than a few micrometers deep into tissue remains challenging. To quantify presynaptic active zones (AZ) of entire, large, conditional detonator hippocampal mossy fiber (MF) boutons with diameters as large as 10 µm, we developed a method for aberration-free volumetricdirectstochastic optical reconstruction microscopy (dSTORM). An optimized protocol for fast repeated axial scanning and efficient sequential labeling of the AZ scaffold Bassoon and membrane bound GFP with Alexa Fluor 647 enables 3D-dSTORM imaging of 25 µm thick mouse brain sections and assignment of AZs to specific neuronal substructures. Quantitative data analysis revealed large differences in Bassoon cluster size and density for distinct hippocampal regions with largest clusters in MF boutons.

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