<title>Measurement of pavement deflection under a moving wheel utilizing scanning laser technology</title>

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

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Ersetzt die Messung der parapapillären Fundusautofluoreszenz mittels modifizierter Fundusfotografie die Scanning-Laser-Ophthalmoskopie?

Klinische Monatsblätter für Augenheilkunde ◽

10.1055/s-0030-1267608 ◽

2010 ◽

Vol 227 (S 02) ◽

Author(s):

R Wienrich ◽

A Langenbucher ◽

W Behrens-Baumann ◽

A Viestenz

Keyword(s):

Scanning Laser

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Circumferential profiles of peripapillary surface height with confocal scanning laser ophthalmoscopy

Korean Journal of Ophthalmology ◽

10.3341/kjo.1997.11.1.7 ◽

1997 ◽

Vol 11 (1) ◽

pp. 7 ◽

Cited By ~ 1

Author(s):

H J Park ◽

Joseph Caprioli

Keyword(s):

Scanning Laser ◽

Scanning Laser Ophthalmoscopy ◽

Confocal Scanning Laser Ophthalmoscopy ◽

Confocal Scanning ◽

Confocal Scanning Laser

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Modern methods of treating rosacea

Medical alphabet ◽

10.33667/2078-5631-2019-1-7(382)-65-71 ◽

2019 ◽

Vol 1 (7) ◽

pp. 65-71

Author(s):

O. A. Egorova ◽

K. A. Novikov

Keyword(s):

Clinical Manifestations ◽

Treatment Method ◽

Current Data ◽

Trigger Factors ◽

Laser Technology ◽

Individual Approach ◽

Modern Methods ◽

Methods Of Treatment ◽

Choice Of Therapy

Presented current data on the etiology of rosacea, the main aspects of pathogenesis, clinical forms of the disease. Reflects trigger factors leading to rosacea, as well as complicating its course. Modern methods of treatment are described, including the use of new safe preparations of ivermectin and brimonidine, providing a good, lasting effect of clinical manifestations of rosacea. The role of laser technology, actively occupying a leading place in the choice of physiotherapeutic treatment method, is noted. The need for an individual approach in the choice of therapy for each patient with rosacea is emphasized.

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