Fluorescence properties of near-infrared tricarbocyanine dyes bound to double-stranded DNA

Author(s):  
Steven A. Soper ◽  
Yolanda Y. Davidson ◽  
Bonnie M. Gunn
2019 ◽  
Vol 943 ◽  
pp. 95-99
Author(s):  
Li Jun Wang ◽  
Kazuo Umemura

Optical absorption spectroscopy provides evidence for individually dispersed carbon nanotubes. A common method to disperse SWCNTs into aqueous solution is to sonicate the mixture in the presence of a double-stranded DNA (dsDNA). In this paper, optical characterization of dsDNA-wrapped HiPco carbon nanotubes (dsDNA-SWCNT) was carried out using near infrared (NIR) spectroscopy and photoluminescence (PL) experiments. The findings suggest that SWCNT dispersion is very good in the environment of DNA existing. Additionally, its dispersion depends on dsDNA concentration.


2012 ◽  
Vol 213 (21) ◽  
pp. 2238-2245 ◽  
Author(s):  
Yuanzhao Wu ◽  
Xuerong Mao ◽  
Xiao Ma ◽  
Xiaobo Huang ◽  
Yixiang Cheng ◽  
...  

2015 ◽  
Vol 13 (16) ◽  
pp. 4727-4732 ◽  
Author(s):  
Orit Redy-Keisar ◽  
Katharina Huth ◽  
Uwe Vogel ◽  
Bernd Lepenies ◽  
Peter H. Seeberger ◽  
...  

Oligoglycerol dendrons effectively enhance the fluorescence properties of a cyanine NIR dye by increasing the solubility in water and the prevention of aggregate formation.


2012 ◽  
Vol 47 (5) ◽  
pp. 585-590 ◽  
Author(s):  
Qingguo Wang ◽  
Liangbi Su ◽  
Hongjun Li ◽  
Lihe Zheng ◽  
Xiaodong Xu ◽  
...  

Author(s):  
Hongyan Sun ◽  
Liu Yang ◽  
Suyuan Chen ◽  
Dong Yi ◽  
Qingxin Chen ◽  
...  

Fluorescent imaging with high temporal and spatial resolution has emerged as one of the most promising strategies to monitor biomolecules and biological processes in living systems. Among many kinds of...


1996 ◽  
Vol 50 (2) ◽  
pp. 211-221 ◽  
Author(s):  
Yolanda Y. Davidson ◽  
Bonnie M. Gunn ◽  
Steven A. Soper

The noncovalent binding and spectroscopic properties of several near-infrared tricarbocyanine dyes with respect to sonicated calf-thymus DNA are reported. The dyes investigated were diethylthiatricarbocyanine iodide (DTTCI), diethyloxatricarbocyanine iodide (DOTCI), and 1,1',3,3,3',3'-hexamethylindotricarbocyanine iodide (HITCI), which are cationic and possess absorption maxima at 772, 695, and 750 nm, respectively, in DMSO. In buffered aqueous solutions, these dyes demonstrated extensive ground-state aggregation in aqueous solvents when compared to DMSO. In the presence of double-stranded DNA (dsDNA), the fluorescence emission spectra revealed enhancement ratios of bound-to-free dye ranging from 4.5 for DOTCI to 128 for DTTCI. Spectrophotometric titrations and Scatchard analyses of the dye-dsDNA complexes yielded nonlinear plots, suggestive of possible multiple binding sites on the DNA. Viscometric titrations of the complexes showed increased solution viscosities for DTTCI, consistent with an unraveling and lengthening of the dsDNA upon complexation. Fluorescence lifetime data of the dye-dsDNA complexes showed longer lifetimes exhibited by these dyes in the presence of the dsDNA compared with those in solutions with no DNA.


2021 ◽  
Vol 39 (No. 1) ◽  
pp. 23-28
Author(s):  
Lijun Wang ◽  
Shusuke Oura ◽  
Yongbo Wu

It is essential to develop a simple method to assess food quality quantitatively. Available methods primarily rely on nanotechnology and offer high selectivity and sensitivity. In this study, we aimed to develop a sensitive nanoprobe, and, to this end, a double-stranded DNA-encased HiPco carbon nanotube (dsDNA-HiPco) hybrid was prepared and used to evaluate the antioxidant potential of a Chinese tea against hydrogen peroxide (H2O2) with a range of irradiation wavelengths. The morphology and dispersion of the hybrids were analysed using atomic force microscopy, which showed that dsDNA wrapped on the SWCNT surface well and homogeneous dispersion of the rod-shaped tubes while the concentration of dsDNA was 1 mg mL–1. The antioxidant effect of Chinese tea was evaluated by using near-infrared absorption and photoluminescence of the hybrid. Experimental results revealed that the tea exerted excellent antioxidant effects when the hybrid was pre-treated with 0.03% wt H2O2. Catechin present in the Chinese tea played a pivotal role in exerting the antioxidant effects. Therefore, a simple detection method proposed herein can be successfully applied in various fields, including biology, medicine, and the food industry.


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