Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency

2014 ◽  
Author(s):  
M. Tamošiūnas ◽  
D. Jakovels ◽  
A. Ļihačovs ◽  
A. Kilikevičius ◽  
J. Baltušnikas ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Multispectral Imaging ◽  
Transfection Efficiency ◽  
Non Invasive

scholarly journals Non-Invasive Technical Investigation of English Portrait Miniatures Attributed to Nicholas Hilliard and Isaac Oliver

Heritage ◽  
2021 ◽  
Vol 4 (3) ◽  
pp. 1165-1181
Author(s):  
Flavia Fiorillo ◽  
Lucia Burgio ◽  
Christine Slottved Kimbriel ◽  
Paola Ricciardi
Keyword(s):  
Fluorescence Spectroscopy ◽  
Scientific Methodology ◽  
Small Scale ◽  
Artistic Practice ◽  
X Ray ◽  
Non Invasive ◽  
Miniature Painting ◽  
Entire Career ◽  
Similarities And Differences ◽  
Victoria And Albert Museum

This study presents the results of the technical investigation carried out on several English portrait miniatures painted in the 16th and 17th century by Nicholas Hilliard and Isaac Oliver, two of the most famous limners working at the Tudor and Stuart courts. The 23 objects chosen for the analysis, spanning almost the entire career of the two artists, belong to the collections of the Victoria and Albert Museum (London) and the Fitzwilliam Museum (Cambridge). A non-invasive scientific methodology, comprising of stereo and optical microscopies, Raman microscopy, and X-ray fluorescence spectroscopy, was required for the investigation of these small-scale and fragile objects. The palettes and working techniques of the two artists were characterised, focusing in particular on the examination of flesh tones, mouths, and eyes. These findings were also compared to the information written in the treatises on miniature painting circulating during the artists’ lifetime. By identifying the materials and techniques most widely employed by the two artists, this study provides information about similarities and differences in their working methods, which can help to understand their artistic practice as well as contribute to matters of attribution.

Exploiting multispectral imaging for non-invasive contamination assessment and mapping of meat samples

Talanta ◽  
2016 ◽  
Vol 161 ◽  
pp. 606-614 ◽  
Author(s):  
Panagiotis Tsakanikas ◽  
Dimitris Pavlidis ◽  
Efstathios Panagou ◽  
George-John Nychas
Keyword(s):  
Multispectral Imaging ◽  
Contamination Assessment ◽  
Non Invasive ◽  
Meat Samples

Towards the DRED of Resin-Supported Combinatorial Libraries: A Non-Invasive Methodology Based on Bead Self-Encoding and Multispectral Imaging

2000 ◽  
Vol 112 (24) ◽  
pp. 4657-4659
Author(s):  
Hicham Fenniri ◽  
Hartmut G. Hedderich ◽  
Kenneth S. Haber ◽  
Jihane Achkar ◽  
Brian Taylor ◽  
...  
Keyword(s):  
Multispectral Imaging ◽  
Combinatorial Libraries ◽  
Non Invasive

scholarly journals MAPPING VIS AND UVL IMAGERY ON 3D GEOMETRY FOR NON-INVASIVE, NON-CONTACT ANALYSIS OF A VASE

2018 ◽  
Vol XLII-2 ◽  
pp. 773-780 ◽  
Author(s):  
E. Nocerino ◽  
D. H. Rieke-Zapp ◽  
E. Trinkl ◽  
R. Rosenbauer ◽  
E. M. Farella ◽  
...  
Keyword(s):  
3D Imaging ◽  
Multispectral Imaging ◽  
3D Models ◽  
Added Value ◽  
Non Invasive ◽  
Comprehensive Knowledge ◽  
3D Geometry ◽  
Modelling Techniques ◽  
The University

The paper presents an investigation about the combination of multispectral and 3D imaging aiming at the analysis of the condition and preservation of an ancient vase. Visible-reflected (VIS) and -induced luminescence (UVL) images are mapped to 3D models produced with image- and range-based 3D modelling techniques. The case study is an Attic vase, part of the pottery collection of the Landesmuseum Rudolfinum (Carinthia, Austria) and temporarily stored in the Institute of Archaeology of the University of Graz, Austria. The aim of this study is to exploit the added-value provided by mapping multispectral imaging onto 3D geometry for a comprehensive knowledge of the condition of a restored Cultural Heritage (CH) item.

Tuning optimum transfection of gemini surfactant–phospholipid–DNA nanoparticles by validated theoretical modeling

Nanoscale ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 1037-1046 ◽  
Author(s):  
Sattar Taheri-Araghi ◽  
Ding-Wen Chen ◽  
Mohammad Kohandel ◽  
Sivabal Sivaloganathan ◽  
Marianna Foldvari
Keyword(s):  
Mathematical Modeling ◽  
Gene Therapy ◽  
Gemini Surfactant ◽  
Transfection Efficiency ◽  
Structural Features ◽  
Coarse Grained ◽  
Viral Gene ◽  
Dna Nanoparticles ◽  
Non Invasive ◽  
Viral Gene Delivery

Coarse-grained mathematical modeling using the polymorphic structural features of gemini nanoparticles assists designing non-viral gene delivery systems with high transfection efficiency for applications in non-invasive gene therapy.

Next generation phenotyping for developing climate resilient rice varieties

2019 ◽  
Vol 56 (Special Issue) ◽  
pp. 92-105
Author(s):  
Rabi N Sahoo ◽  
C Viswanathan ◽  
Gopal Krishna ◽  
Bappa Das ◽  
Swati Goel ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Water Deficit ◽  
Multispectral Imaging ◽  
Sugar Content ◽  
Stress Index ◽  
Water Deficit Stress ◽  
Next Generation ◽  
Crop Water Stress Index ◽  
Non Invasive ◽  
Rice Genotypes

Present paper deals with different components of next generation phenomics for characterizing rice genotypes for water deficit stress. Major sensors used in the study were non-imaging hyperspectal remote sensing, thermal imaging at ground platform and RGB and multispectral imaging sensors from drone platform. Different spectral indices were evaluated along with new proposed index and different multivariate models were studied for non-invasive estimation of relative water content (RWC) and sugar content in rice plant using spectral reflectance data collected in spectral range 350 to 2500 nm. Spectral data were further used for spectral discrimination of rice genotypes. Crop water stress index derived from thermal images acquired for rice genotypes could well characterize the drought resistant and sensitive genotypes. Initial study on field phenotyping through drone remote sensing using multispectral and RGB sensor was also explored to capture differential response of genotypes, trait and heat map mapping. All developed protocols as reliable alternative to conventional methods are fast, economic and non-invasive and in use in plant phenomics centre for high throughput plant phenotyhping for water deficit stress studies.

scholarly journals Spectral Behavior of White Pigment Mixtures Using Reflectance, Ultraviolet—Fluorescence Spectroscopy, and Multispectral Imaging

2017 ◽  
Vol 71 (12) ◽  
pp. 2616-2625 ◽  
Author(s):  
Lucilla Pronti ◽  
Anna Candida Felici ◽  
Matthieu Ménager ◽  
Cathy Vieillescazes ◽  
Mario Piacentini
Keyword(s):  
Fluorescence Spectroscopy ◽  
Multispectral Imaging ◽  
Linseed Oil ◽  
Fluorescence Emission ◽  
Reflectance Spectroscopy ◽  
Lead White ◽  
Spectral Behavior ◽  
Uv Fluorescence ◽  
Zinc White ◽  
Titanium White

Reflectance spectroscopy, ultraviolet (UV)—fluorescence spectroscopy, and multispectral imaging have been widely employed for pigment identification on paintings. From ancient times to the present, lead white, zinc white, and titanium white have been the most important white pigments used for paintings and they are used as pigment markers for dating a work of art. The spectral behavior of these pigments is reported in several scientific papers and websites, but those of their mixtures are quite unknown. We present a combined nondestructive approach for identifying mixtures of lead white, zinc white, and titanium white as powder and dispersed in two different binder media (egg yolk and linseed oil) by using reflectance spectroscopy, spectrofluorimetry, multispectral reflectance and UV-fluorescence imaging. We propose a novel approach for mapping the presence of white pigments in paintings by false color images obtained from multispectral reflectance and UV-fluorescence images. We found that the presence of lead white mixed with either zinc white or titanium white is highly detectable. Zinc white mixed with lead white or titanium white can be identified due to its UV-fluorescence emission, whereas titanium white in association with lead white or zinc white is distinguishable by its reflectance spectral features. In most cases, the UV-fluorescence analyses also permit the recognition of the binder media in which the pigments are dispersed.

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