Sorting on the basis of deformability of single cells in a femtosecond laser fabricated optofluidic device

2015 ◽  
Author(s):  
F. Bragheri ◽  
P. Paiè ◽  
T. Yang ◽  
G. Nava ◽  
R. Martìnez Vàzquez ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cells

scholarly journals Photoporation of Biomolecules into Single Cells in Living Vertebrate Embryos Induced by a Femtosecond Laser Amplifier

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27677 ◽  
Author(s):  
Yoichiroh Hosokawa ◽  
Haruki Ochi ◽  
Takanori Iino ◽  
Akihiro Hiraoka ◽  
Mikiko Tanaka
Keyword(s):  
Femtosecond Laser ◽  
Single Cells ◽  
Laser Amplifier ◽  
Vertebrate Embryos

scholarly journals Femtosecond laser fabricated monolithic chip for optical trapping and stretching of single cells

2010 ◽  
Vol 18 (5) ◽  
pp. 4679 ◽  
Author(s):  
N. Bellini ◽  
K. C. Vishnubhatla ◽  
F. Bragheri ◽  
L. Ferrara ◽  
P. Minzioni ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Optical Trapping ◽  
Single Cells

Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

2010 ◽  
Vol 13 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Yasuyo Maezawa ◽  
Kazunori Okano ◽  
Mie Matsubara ◽  
Hiroshi Masuhara ◽  
Yoichiroh Hosokawa
Keyword(s):  
Cell Differentiation ◽  
Femtosecond Laser ◽  
Single Cells ◽  
Impulsive Force ◽  
Morphological Evaluation

Trapping and Stretching of Single Cells in an Optofluidic Chip Fabricated by a Femtosecond Laser

2010 ◽  
Author(s):  
N. Bellini ◽  
K. Vishnubhatla ◽  
R. Ramponi ◽  
R. Osellame ◽  
F. Bragheri ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cells ◽  
Optofluidic Chip

Femtosecond laser fabricated integrated chip for manipulation of single cells (Conference Presentation)

2016 ◽  
Author(s):  
Anusha Keloth ◽  
Melanie Jimenez ◽  
Helen Bridle ◽  
Lynn Paterson ◽  
Gerard H. Markx ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cells

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

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