Effects of cholesterol on plasma membrane lipid order in MCF-7 cells by two-photon microscopy

2014 ◽  
Author(s):  
Yixiu Zeng ◽  
Jianling Chen ◽  
Hongqin Yang ◽  
Yuhua Wang ◽  
Hui Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Lipid ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lipid Order ◽  
Plasma Membrane Lipid ◽  
Mcf 7

ADH modulates plasma membrane lipid order of living MDCK cells via a cAMP-dependent process

1990 ◽  
Vol 259 (1) ◽  
pp. F95-F103
Author(s):  
M. C. Giocondi ◽  
G. Friedlander ◽  
C. Le Grimellec
Keyword(s):  
Plasma Membrane ◽  
Mdck Cells ◽  
Membrane Lipid ◽  
Target Cells ◽  
Similar Response ◽  
Solute Permeability ◽  
Lipid Order ◽  
Plasma Membrane Lipid ◽  
Darby Canine Kidney

Using 1-[4-(trimethylamino)phenyl]-6-phenyl-hexa-1,3,5-triene, a fluorescent probe that specifically labels the external leaflet of the plasma membrane of living cells, we examined the effects of antidiuretic hormone (ADH) and various agents known to raise intracellular adenosine 3',5'-cyclic monophosphate (cAMP) on the physical state of the plasma membrane of Madin-Darby canine kidney (MDCK) cells. In polarized cells grown as a monolayer, [desamino-Cys1, DArg8]-vasopressin (V2-agonist) elicited a biphasic decrease in the lipid order as estimated from the decrease in fluorescence anisotropy (from r = 0.317 to r = 0.304, 37 degrees C) of the apical domain of the plasma membrane, equivalent at the peak response (t = 5 min) to that produced by an upward shift in temperature of 5-6 degrees C. A similar response was obtained by adding dibutyryl cAMP to the monolayers. Experiments on cell suspensions further indicated that the biphasic decrease in lipid order could also be evoked by forskolin, prostaglandin E2, and bradykinin but not by bradykinin plus indomethacin and was inhibitable by the protein kinases inhibitor compound H7. These data demonstrate that the lipid order of the plasma membrane of MDCK cells can be modulated in situ by cAMP-dependent processes probably involving protein kinase A activity, i.e., that membrane “fluidity” might act in the regulation of the cellular function of living epithelial cells. They provide a rationale for the changes in lipophilic solute permeability that accompany the increase in water permeability of target cells on ADH administration.

scholarly journals High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells

2010 ◽  
Vol 27 (4-6) ◽  
pp. 178-189 ◽  
Author(s):  
Dylan M. Owen ◽  
Stephane Oddos ◽  
Sunil Kumar ◽  
Daniel M. Davis ◽  
Mark A. A. Neil ◽  
...  
Keyword(s):  
T Cells ◽  
Plasma Membrane ◽  
Immunological Synapse ◽  
High Plasma ◽  
Membrane Lipid ◽  
Lipid Order ◽  
Plasma Membrane Lipid

Plasma membrane lipid order of leukemic and normal immature avian erythroid cells

1984 ◽  
Vol 153 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Gary Price Cofer ◽  
Patrick Williamson ◽  
Robert A. Schlegel
Keyword(s):  
Plasma Membrane ◽  
Membrane Lipid ◽  
Erythroid Cells ◽  
Lipid Order ◽  
Plasma Membrane Lipid

Dietary fat ratios and liver plasma membrane lipid composition

Lipids ◽  
1988 ◽  
Vol 23 (9) ◽  
pp. 829-833 ◽  
Author(s):  
Michael W. Hamm ◽  
Anna Sekowski ◽  
Roni Ephrat
Keyword(s):  
Plasma Membrane ◽  
Lipid Composition ◽  
Dietary Fat ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Liver Plasma Membrane ◽  
Plasma Membrane Lipid

Alterations in plasma membrane lipid organization during lymphocyte differentiation

1986 ◽  
Vol 126 (3) ◽  
pp. 379-388 ◽  
Author(s):  
Brian J. Del Buono ◽  
Patrick L. Williamson ◽  
Robert A. Schlegel
Keyword(s):  
Plasma Membrane ◽  
Membrane Lipid ◽  
Lymphocyte Differentiation ◽  
Plasma Membrane Lipid ◽  
Lipid Organization

1P-0227 Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages

2003 ◽  
Vol 4 (2) ◽  
pp. 69 ◽  
Author(s):  
W. Jessup ◽  
K. Gaus ◽  
L. Kritharides ◽  
A. Boettcher ◽  
W. Drobnik ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Apolipoprotein A ◽  
Plasma Membrane Lipid
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