The synchronous fluorescence spectra character of hypocrellin B with type I collagen under irradiation

2014 ◽  
Author(s):  
Jucheng Zhang ◽  
Heping Yan ◽  
Mengting Yang ◽  
Yongzhen Feng ◽  
Yun Huang ◽  
...  
Keyword(s):  
Fluorescence Spectra ◽  
Type I Collagen ◽  
Synchronous Fluorescence ◽  
Type I ◽  
Synchronous Fluorescence Spectra ◽  
Hypocrellin B

scholarly journals Synchronous fluorescence spectra of water contaminated by dispersed crude oil

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Emilia Baszanowska ◽  
Zbigniew Otremba
Keyword(s):  
Crude Oil ◽  
Fluorescence Spectra ◽  
Oil Pollution ◽  
Synchronous Fluorescence ◽  
Mining Equipment ◽  
Oil Emulsion ◽  
Synchronous Fluorescence Spectra ◽  
Emulsion Concentration ◽  
Petroleum Extraction

The development of petroleum extraction and transport technology does not ensure complete isolation of these substances from the natural environment. This problem is exacerbated by the location of mining equipment on the sea shelf and the fact that numerous submarine pipelines, tankers and handling terminals can also emit oil pollution. Therefore, the possibility of detecting oil dispersed in the water is particularly important. This paper reports the efforts to identify methods of characterization of the water containing the crude oil emulsion in a very low concentration (a few to several tens of ppm). Due to this, the effect of emulsion concentration on the possibility of its objective characterization using synchronous fluorescence spectra was studied. The similarity of spectra at various oil concentrations was analysed. It has been shown that the stabilization of the shape of synchronous fluorescence spectra occurs at relatively low oil concentrations.

Using fluorescence quenching combined with two-dimensional correlation fluorescence spectroscopy to characterise the binding-site heterogeneity of dissolved organic matter with copper and mercury in lake sediments

2017 ◽  
Vol 14 (2) ◽  
pp. 91 ◽  
Author(s):  
Xu-jing Guo ◽  
Yun-zhen Li ◽  
Yan-hong Feng ◽  
Dong-hai Yuan
Keyword(s):  
Organic Matter ◽  
Fluorescence Quenching ◽  
Binding Site ◽  
Fluorescence Spectra ◽  
Correlation Spectroscopy ◽  
Synchronous Fluorescence ◽  
Two Dimensional ◽  
Synchronous Fluorescence Spectra ◽  
Site Heterogeneity ◽  
Different Sources

Environmental contextDissolved organic matter (DOM) plays an important role in influencing the migration and ultimate fate of metals. Different sources of pollution resulted in changes in the structure of sediment DOM in Lake Wuliangsuhai. We investigate the binding properties of DOM with CuII and HgII using fluorescence quenching combined with two-dimensional correlation spectroscopy (2D-COS) in order to demonstrate the influence of different sources of DOM on metals. AbstractDissolved organic matter (DOM) was collected from three sampling sites (L1, L2 and L3) in Lake Wuliangsuhai. L1 received upstream industrial wastewater and domestic sewage. L2 had suffered from agricultural non-point source pollution. L3 was situated in the lake outlet area. The complexation of DOM with CuII and HgII was investigated based on fluorescence quenching of the synchronous fluorescence spectra on the addition of CuII and on two-dimensional correlation spectroscopy (2D-COS). The synchronous and asynchronous maps derived from 2D-COS provided a clear picture of the heterogeneous distribution of CuII and HgII binding sites, which was not readily detected using only the synchronous fluorescence spectra. CuII and HgII complexation was stronger at shorter wavelengths than at longer wavelengths. Moreover, fluorescence quenching also occurred intensely in the fulvic-like regions (363nm for DOM-Cu in L2 and 365nm for DOM-Hg in L1). The logarithms of the stability constants (log KM) ranged from 4.42 to 6.23, from 4.75 to 4.86, and from 4.80 to 5.73 for DOM-Cu in L1, L2 and L3, respectively, depending on the wavelength. DOM at the longer wavelengths exhibited a higher log KM than that at the shorter wavelengths, and the f values in the protein-like region were clearly high. These results suggest that the influence of the structural and chemical properties of DOM on CuII binding may differ for DOM from different sources. The combined approach of fluorescence quenching and 2D-COS could be applied as a tool for evaluating the metal binding site heterogeneity of DOM.

scholarly journals Intraspecific discrimination of fish populations by fluorescence spectroscopy

2021 ◽  
Vol 43 ◽  
pp. e48395
Author(s):  
Dinorah Machado Vaz de Lima ◽  
Cristiane Ávila Santana ◽  
Luis Humberto da Cunha Andrade ◽  
Yzel Rondon Súarez ◽  
Sandro Marcio Lima
Keyword(s):  
Fluorescence Spectroscopy ◽  
Fluorescence Spectra ◽  
Diet Composition ◽  
Type I Collagen ◽  
Environmental Study ◽  
Fluorescence Signal ◽  
Type I ◽  
Genetic Isolation ◽  
Multivariate Statistical ◽  
Local Conditions

This study used the visible fluorescence signal of scales from Astyanax lacustris fishes to differentiate ten populations of streams in Ivinhema River Basin, Upper Paraná Basin, Brazil. Scales were removed from the humeral region of each fish and the fluorescence spectroscopy was carried out with two excitation wavelengths: at 360 nm (UV-A) and 405 nm. The broad emission covers all visible regions and it is related to the organic fraction of scale, basically composed from type I collagen. By interpreting the experimental fluorescence spectra with multivariate statistical analysis, it was possible to discriminate the investigated populations. By exciting the inner face of scales at 405 nm, for instance, the obtained Wilk’s lambda was 0.143, and the ten sampled streams could be statistically differentiated with 85.2% of explanation. This fluorescence interpretation exhibits very good correlation with the diet composition, which was also investigated for the same fishes from which the scales were removed. The applied methodology was capable to analyze the scales of A. lacustris, and to provide meaningful and enlightening results for the differentiation of populations. This methodology is very important for aquatic environmental study, mainly because small fishes, non-migratory or with small migration rate, can exhibit differences among habitats, as response to genetic isolation and adjustment to local conditions.

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