Highly sensitive detection of human papillomavirus type 16 DNA using time-resolved fluorescence microscopy and long lifetime probes

1995 ◽  
Author(s):  
Xue F. Wang ◽  
Ammasi Periasamy ◽  
Pawel Wodnicki ◽  
M. Siadat-Pajouh ◽  
Brian Herman
Keyword(s):  
Human Papillomavirus ◽  
Fluorescence Microscopy ◽  
Sensitive Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Human Papillomavirus Type 16 ◽  
Highly Sensitive ◽  
Long Lifetime ◽  
Highly Sensitive Detection

Time-resolved fluorescence of a new europium-chelate complex: demonstration of highly sensitive detection of protein and DNA samples

1993 ◽  
Vol 115 (23) ◽  
pp. 11032-11033 ◽  
Author(s):  
Ashis K. Saha ◽  
Kathryn Kross ◽  
Edmond D. Kloszewski ◽  
Donald A. Upson ◽  
John L. Toner ◽  
...  
Keyword(s):  
Chelate Complex ◽  
Sensitive Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Highly Sensitive ◽  
Europium Chelate ◽  
Highly Sensitive Detection

Eu3+‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B1in feed

2017 ◽  
Vol 98 (2) ◽  
pp. 674-680 ◽  
Author(s):  
Xiaofei Hu ◽  
Jingjing Yao ◽  
Fangyu Wang ◽  
Mengqi Yin ◽  
Yaning Sun ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Time Resolved ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Highly sensitive detection of the human papillomavirus E6 protein by DNA-protected silver nanoclusters and the intrinsic mechanism

2019 ◽  
Vol 43 (37) ◽  
pp. 14944-14951
Author(s):  
Xiang-Yu Jia ◽  
Ya-Rong Xue ◽  
Chun-Xia Zhang ◽  
Quan Luo ◽  
Yuqing Wu
Keyword(s):  
Human Papillomavirus ◽  
Early Diagnosis ◽  
Silver Nanoclusters ◽  
Sensitive Detection ◽  
Biological Application ◽  
Intrinsic Mechanism ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
E6 Protein

The present study not only supplies a good approach for the early diagnosis of HPV-related cancer but also enriches the biological application of AgNCs–dsDNA.

Ultrasensitive Thyrotropin Immunoassay Based on Enzymatically Amplified Time-Resolved Fluorescence with a Terbium Chelate

1992 ◽  
Vol 38 (4) ◽  
pp. 545-548 ◽  
Author(s):  
A Papanastasiou-Diamandi ◽  
T K Christopoulos ◽  
E P Diamandis
Keyword(s):  
Alkaline Phosphatase ◽  
Detection Limit ◽  
Solid Phase ◽  
Thyroid Stimulating Hormone ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Highly Sensitive ◽  
Assay Time ◽  
Highly Sensitive Detection

Abstract We describe an ultrasensitive, enzymatically amplified time-resolved fluorescence immunoassay of thyrotropin (thyroid-stimulating hormone) in serum with use of a terbium chelate as the detectable moiety. In this assay, thyrotropin is first simultaneously reacted with a solid-phase (microtiter well) monoclonal antibody and a soluble biotinylated monoclonal detection antibody. After washing, a streptavidin-alkaline phosphatase conjugate is added, followed by another washing. Alkaline phosphatase acts on the substrate 5-fluorosalicyl phosphate (FSAP) to produce 5-fluorosalicylic acid (FSA). FSA, but not FSAP, can then form with Tb3+ and EDTA a highly fluorescent ternary complex of long fluorescence lifetime. This complex is quantified with time-resolved fluorometry. The thyrotropin assay is highly sensitive (detection limit approximately 0.003 milli-int. unit/L when a total assay time of 85 min is used), precise, and accurate. The thyrotropin assay can also be completed in less than 30 min (detection limit 0.013 milli-int. unit/L), thus making this procedure a candidate technology for high-throughput automated analyzers.

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