Three-dimensional Fourier analysis methods for digital processing and 3D visualization of confocal transmission images

1995 ◽  
Author(s):  
Carol J. Cogswell ◽  
Kieran G. Larkin ◽  
Matthew R. Arnison ◽  
John W. O'Byrne
Keyword(s):  
Fourier Analysis ◽  
3D Visualization ◽  
Three Dimensional ◽  
Digital Processing ◽  
Analysis Methods

The Influence of Blade Wakes on the Performance of Interturbine Diffusers

1996 ◽  
Vol 118 (2) ◽  
pp. 347-352 ◽  
Author(s):  
R. G. Dominy ◽  
D. A. Kirkham
Keyword(s):  
Performance Modeling ◽  
Three Dimensional ◽  
Secondary Flows ◽  
Two Dimensional ◽  
Correct Performance ◽  
Analysis Methods ◽  
Flow Factor ◽  
The Mean ◽  
Lp Turbine

Interturbine diffusers provide continuity between HP and LP turbines while diffusing the flow upstream of the LP turbine. Increasing the mean turbine diameter offers the potential advantage of reducing the flow factor in the following stages, leading to increased efficiency. The flows associated with these interturbine diffusers differ from those in simple annular diffusers both as a consequence of their high-curvature S-shaped geometry and of the presence of wakes created by the upstream turbine. It is shown that even the simplest two-dimensional wakes result in significantly modified flows through such ducts. These introduce strong secondary flows demonstrating that fully three-dimensional, viscous analysis methods are essential for correct performance modeling.

scholarly journals Virtual Archaeology of Death and Burial: A Procedure for Integrating 3D Visualization and Analysis in Archaeothanatology

2021 ◽  
Vol 7 (1) ◽  
pp. 540-555
Author(s):  
Hayley L. Mickleburgh ◽  
Liv Nilsson Stutz ◽  
Harry Fokkens
Keyword(s):  
Spatial Information ◽  
3D Visualization ◽  
Rapid Development ◽  
Three Dimensional ◽  
3D Models ◽  
The Body ◽  
Test Case ◽  
Archaeology Of Death ◽  
Human Burials ◽  
3D Information

Abstract The reconstruction of past mortuary rituals and practices increasingly incorporates analysis of the taphonomic history of the grave and buried body, using the framework provided by archaeothanatology. Archaeothanatological analysis relies on interpretation of the three-dimensional (3D) relationship of bones within the grave and traditionally depends on elaborate written descriptions and two-dimensional (2D) images of the remains during excavation to capture this spatial information. With the rapid development of inexpensive 3D tools, digital replicas (3D models) are now commonly available to preserve 3D information on human burials during excavation. A procedure developed using a test case to enhance archaeothanatological analysis and improve post-excavation analysis of human burials is described. Beyond preservation of static spatial information, 3D visualization techniques can be used in archaeothanatology to reconstruct the spatial displacement of bones over time, from deposition of the body to excavation of the skeletonized remains. The purpose of the procedure is to produce 3D simulations to visualize and test archaeothanatological hypotheses, thereby augmenting traditional archaeothanatological analysis. We illustrate our approach with the reconstruction of mortuary practices and burial taphonomy of a Bell Beaker burial from the site of Oostwoud-Tuithoorn, West-Frisia, the Netherlands. This case study was selected as the test case because of its relatively complete context information. The test case shows the potential for application of the procedure to older 2D field documentation, even when the amount and detail of documentation is less than ideal.

Macromolecular Structure Visualization Tools at NCMI

2000 ◽  
Vol 6 (S2) ◽  
pp. 282-283
Author(s):  
Matthew Dougherty ◽  
Wah Chiu
Keyword(s):  
Human Factors ◽  
3D Visualization ◽  
Three Dimensional ◽  
Theoretical Models ◽  
Biological Research ◽  
Macromolecular Structure ◽  
X Ray ◽  
Visualization Tools ◽  
Types Of Information ◽  
3D Em

Sophisticated tools are needed to examine the results of cyro-microscopy. As the size and resolution of three dimensional macromolecular structures steadily improve, and the speed at with which they can be generated increases, researchers are finding they are inundated with larger datasets and at the same time are compelled to expediently evaluate these structures in unforeseen ways. Integration of EM data with other types of information is becoming necessary and routine; for example X-ray data, 3D EM reconstructions, and theoretical models, must be evaluated in concert to discount or propose hypothesis. To create such tools, the developer must take into account not only the empirical and theoretical possibilities, but also they must master the human factors and computational limits. During the last five years, the National Center for Macromolecular Imaging (NCMI) has progressed from a remedial 3D visualization capability to a collection of visualization tools allowing researchers to focus on the discovery phase of biological research.

A novel three-dimensional visualization system revealed an essential adaptive angiogenic response during the early phase of pulmonary hypertension

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
T Fujiwara ◽  
N Takeda ◽  
M Hatano ◽  
S Nishimura ◽  
I Komuro
Keyword(s):  
Pulmonary Hypertension ◽  
Early Phase ◽  
3D Visualization ◽  
Hypoxia Inducible Factor ◽  
Three Dimensional ◽  
Right Heart Failure ◽  
Wall Thickening ◽  
Funding Source ◽  
Vegf Expression ◽  
Visualization System

Abstract Background Pulmonary hypertension (PH) is characterized by increased pulmonary vascular resistance and right heart failure with progressive narrowing or occlusion of the pulmonary artery. However, the assessment of vascular remodeling is mostly limited to averaged increases in wall thickening, and even the role of vascular endothelial growth factor (VEGF), remains incompletely understood; Although abundantly expressed VEGF is expected to elicit angio-obliteration and the knockout of hypoxia inducible factor (HIF) prevents PH in mice, VEGF inhibitor Sugen exacerbates hypoxia (Hx)-induced PH model, which is referred to as VEGF paradox. Purpose To analyze three-dimensional (3D) spatiotemporal changes of pulmonary microstructure and function, which reflect the disease activity and lead to resolve the paradox. Methods and results We developed a novel 3D visualization system of microstructural networks in whole mouse organ with single-cell resolution, using combined tissue clearing technique called CUBIC and multiphoton excitation microscope. The system enabled the simultaneous 3D evaluation of microvascular structure, invaded macrophages and fibrosis with effective penetration of several mm (whole organ). Three-dimensional observations of PH mice models including Hx, Sugen/Hx, and human-like Alk1+/− hereditary PH models, revealed that not only inward (negative) microvessel remodeling with stenosis, but also marked elongation of microvascular ECs, was evident except Sugen/Hx model at the early phase, which had not been detected by 2D histological sections. Comparable transcriptome analysis revealed that PGC1α, which regulates HIF-independent VEGF expression and angiogenesis, plays an important role in the characteristic response for mitochondrial and microvascular maintenance. PGC1α was up-regulated in the early phage in Hx and Alk1+/− PH models with microvascular angiogenetic change, whereas Sugen/Hx-model did not increase PGC1α expression and did not show microvascular remodeling. Furthermore pulmonary ECs-specific PGC1α-deficient mice exacerbated Hx-PH model with decreased VEGF expression and microvessel density, and administration of Baicalin, a flavonoid enhancing PGC1α expression, ameliorated Hx-PH model with increased VEGF expression. Conclusions The 3D visualization system disclosed an unexpected change of angiogenic microvascular structure in the early phage of PH, which is regulated by EC PGC1α. Microvascular angiogenesis which is induced by up-regulation in PGC1α -VEGF pathway is a crucial factor for compensation of PH in the early phase, which provides a potential novel therapeutic target for PH. Figure 1 Funding Acknowledgement Type of funding source: Public grant(s) – National budget only. Main funding source(s): JSJP

Evaluating the Effect of Three-Dimensional Visualization on Force Application and Performance Time during Robotics-Assisted Mitral Valve Repair

2013 ◽  
Vol 8 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Maria E. Currie ◽  
Ana Luisa Trejos ◽  
Reiza Rayman ◽  
Michael W.A. Chu ◽  
Rajni Patel ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Mitral Valve ◽  
Haptic Feedback ◽  
3D Visualization ◽  
Three Dimensional ◽  
Test Bed ◽  
Mitral Valve Annuloplasty ◽  
Significant Difference ◽  
The Mean ◽  
Mean Time

Objective The purpose of this study was to determine the effect of three-dimensional (3D) binocular, stereoscopic, and two-dimensional (2D) monocular visualization on robotics-assisted mitral valve annuloplasty versus conventional techniques in an ex vivo animal model. In addition, we sought to determine whether these effects were consistent between novices and experts in robotics-assisted cardiac surgery. Methods A cardiac surgery test-bed was constructed to measure forces applied during mitral valve annuloplasty. Sutures were passed through the porcine mitral valve annulus by the participants with different levels of experience in robotics-assisted surgery and tied in place using both robotics-assisted and conventional surgery techniques. Results The mean time for both the experts and the novices using 3D visualization was significantly less than that required using 2D vision (P < 0.001). However, there was no significant difference in the maximum force applied by the novices to the mitral valve during suturing (P = 0.7) and suture tying (P = 0.6) using either 2D or 3D visualization. The mean time required and forces applied by both the experts and the novices were significantly less using the conventional surgical technique than when using the robotic system with either 2D or 3D vision (P < 0.001). Conclusions Despite high-quality binocular images, both the experts and the novices applied significantly more force to the cardiac tissue during 3D robotics-assisted mitral valve annuloplasty than during conventional open mitral valve annuloplasty. This finding suggests that 3D visualization does not fully compensate for the absence of haptic feedback in robotics-assisted cardiac surgery.

scholarly journals Nervous Activity of the Brain in Five Dimensions

Biophysica ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 38-47
Author(s):  
Arturo Tozzi ◽  
James F. Peters ◽  
Norbert Jausovec ◽  
Arjuna P. H. Don ◽  
Sheela Ramanna ◽  
...  
Keyword(s):  
Fourier Analysis ◽  
Nervous Activity ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Data Sets ◽  
Spatial And Temporal Patterns ◽  
Five Dimensions ◽  
Four Dimensions ◽  
Scale Free ◽  
The Brain

The nervous activity of the brain takes place in higher-dimensional functional spaces. It has been proposed that the brain might be equipped with phase spaces characterized by four spatial dimensions plus time, instead of the classical three plus time. This suggests that global visualization methods for exploiting four-dimensional maps of three-dimensional experimental data sets might be used in neuroscience. We asked whether it is feasible to describe the four-dimensional trajectories (plus time) of two-dimensional (plus time) electroencephalographic traces (EEG). We made use of quaternion orthographic projections to map to the surface of four-dimensional hyperspheres EEG signal patches treated with Fourier analysis. Once achieved the proper quaternion maps, we show that this multi-dimensional procedure brings undoubted benefits. The treatment of EEG traces with Fourier analysis allows the investigation the scale-free activity of the brain in terms of trajectories on hyperspheres and quaternionic networks. Repetitive spatial and temporal patterns undetectable in three dimensions (plus time) are easily enlightened in four dimensions (plus time). Further, a quaternionic approach makes it feasible to identify spatially far apart and temporally distant periodic trajectories with the same features, such as, e.g., the same oscillatory frequency or amplitude. This leads to an incisive operational assessment of global or broken symmetries, domains of attraction inside three-dimensional projections and matching descriptions between the apparently random paths hidden in the very structure of nervous fractal signals.

Empirically validating five propositions regarding 3D visualizations for subsurface utility projects

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mechiel van Manen ◽  
Léon olde Scholtenhuis ◽  
Hans Voordijk
Keyword(s):  
Construction Projects ◽  
3D Visualization ◽  
Three Dimensional ◽  
Urban Environments ◽  
Content Type ◽  
Complexity Level ◽  
Daily Work ◽  
Complex Projects ◽  
Project Complexity ◽  
Multiple Case

PurposeThis study aims to empirically validate five propositions about the benefits of three-dimensional (3D) visualizations for the management of subsurface utility projects. Specifically, the authors validate whether benefits from 3D in the literature of building construction project management also apply to subsurface utility projects and map them using a taxonomy of project complexity levels.Design/methodology/approachA multiple case study of three utility construction projects was carried out during which the first author was involved in the daily work practices at a utility contractor. 3D visualizations of existing project models were developed, and design and construction meetings were conducted. Practitioners' interactions with and reflections on these 3D visualizations were noted. Observational data from the three project types were matched with the five propositions to determine where benefits of 3D visualizations manifested themselves.FindingsPractitioners found that 3D visualizations had most merit in crowded urban environments when constructing rigid pipelines. All propositions were validated and evaluated as beneficial in subsurface utility projects of complexity level C3. It is shown that in urban projects with rigid pipelines (project with the highest complexity level), 3D visualization prevents misunderstanding or misinterpretations and increases efficiency of coordination. It is recommended to implement 3D visualization approaches in such complex projectsOriginality/valueThere is only limited evidence on the value 3D visualizations in managing utility projects. This study contributes rich empirical evidence on this value based on a six-month observation period at a subsurface contractor. Their merit was assessed by associating 3D approaches with project complexity levels, which may help utility contractors in strategically implementing 3D applications.

scholarly journals POSSIBILITIES OF THE APPLICATION OF THREE-DIMENSIONAL TECHNOLOGIES AND MODERN TECHNOLOGICAL MEANS IN THE CRIME INVESTIGATION ACTIVITY

2018 ◽  
Vol 18 ◽  
pp. 98-105
Author(s):  
N. V. Pavliuk
Keyword(s):  
Digital Media ◽  
Technology Use ◽  
Laser Scanning ◽  
3D Model ◽  
Imaging System ◽  
3D Visualization ◽  
Three Dimensional ◽  
Scanning System ◽  
Crime Reconstruction ◽  
Technological Means

The issues related to the introduction of innovative methods, technologies and technological means in the investigation of crimes are considered. It is noted that one of the main directions of the development of Criminalistics is the assimilation of the virtual reality associated with computerization of spheres of life, implementation of modern technologies and their use in law enforcement. Technology use of laser scanning of terrain and objects resulting in 3D model is produced allows several times to increase informative value of data collected at the incident scene, provides a visual and convenient visualization in three-dimensional form. As against photo and video images, 3D model has a stereoscopic image and the ability to freely change the angle while viewing. Besides to scanning results can be stored on any digital media without the possibility of changes or adjustments. Attention is focused on the technological capabilities of 3D-visualization systems on examples of their use in foreign countries as technological means of capturing the situation of the scene and the subsequent of a crime reconstruction. Thus, using a portable three-dimensional imaging system for working with volumetric traces at a crime scene, it is possible to obtain accurate three-dimensional images of traces of protectors or footprints (shoes) on soil and snow. This system is an alternative to traditional methods of fixing evidence: photofixing and making plaster casts. Unlike other systems, new approach does not require the use of lasers. The expediency of expanding the range of 3D laser scanning system use in modern investigative and judicial practice of our state with the aim of increasing the level of provision of pre-trial investigation authorities with technological means and bringing it closer to European standards is argued.

scholarly journals 3D visualization in otosurgery

2020 ◽  
Vol 19 (6) ◽  
pp. 38-49
Author(s):  
D. M. Kuz’min ◽  
◽  
A. A. Fedotova ◽  
Keyword(s):  
Middle Ear ◽  
3D Visualization ◽  
Research Work ◽  
Three Dimensional ◽  
Middle Ear Surgery ◽  
Ear Surgery ◽  
Task Load ◽  
Additional Information ◽  
Surgical Field ◽  
Nasa Task Load Index

The main priority of middle ear surgery is to create a safe and optimal view of the surgical field, as well as the most accurate visualization of anatomical structures, which is a driving factor in the evolution of otosurgery. The additional information provided by three-dimensional (3D) images has been proven to improve understanding of the temporal bone anatomy and improve the operator’s ability to assess associated diseases, thereby optimizing surgical management. In the presented experimental research work, a new technique for visualizing the surgical field is described, which improves the quality of the operator’s work and expands the possibilities of middle ear surgery. On the basis of the Chair of Otorhinolaryngology of the Mechnikov North-Western State Medical University a remote adapter for an endoscopic tube was created, which allows you to broadcast the video image received from its distal end to virtual reality glasses. For a detailed understanding of the principle of information transmission in a new three-dimensional reality, we used concepts such as disparity and stereopsis. All research results were evaluated according to the NASA Task Load Index scale. Analyzing the results of the experiment, in the conditions of three-dimensional visualization of the surgical field, a lower level of subjective workload was revealed, which was regarded as a positive effect of the realization of the phenomenon of stereopsis, when performing manipulations on the middle ear.

scholarly journals 3D visualization of macromolecule synthesis

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Timothy J Duerr ◽  
Ester Comellas ◽  
Eun Kyung Jeon ◽  
Johanna E Farkas ◽  
Marylou Joetzjer ◽  
...  
Keyword(s):  
3D Visualization ◽  
Fluorescent Microscopy ◽  
Three Dimensional ◽  
Light Sheet ◽  
Macromolecular Synthesis ◽  
Volumetric Imaging ◽  
Processing Pipeline ◽  
Powerful Means

Measuring nascent macromolecular synthesis in vivo is key to understanding how cells and tissues progress through development and respond to external cues. Here we perform in vivo injection of alkyne- or azide-modified analogs of thymidine, uridine, methionine, and glucosamine to label nascent synthesis of DNA, RNA, protein, and glycosylation. Three-dimensional volumetric imaging of nascent macromolecule synthesis was performed in axolotl salamander tissue using whole-mount click chemistry-based fluorescent staining followed by light sheet fluorescent microscopy. We also developed an image processing pipeline for segmentation and classification of morphological regions of interest and individual cells, and we apply this pipeline to the regenerating humerus. We demonstrate our approach is sensitive to biological perturbations by measuring changes in DNA synthesis after limb denervation. This method provides a powerful means to quantitatively interrogate macromolecule synthesis in heterogenous tissues at the organ, cellular, and molecular levels of organization.

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